What makes the filtration process superior to standard 0.22 micron filtered media?

FluxMPS uses four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II - Polish, two dedicated prefilter/final-filter pairs reaching a 0.04 micron final cut-off versus a single 0.22 micron pass in conventional media.

FluxMPS Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Q: Why are sodium bicarbonate and phenol red both excluded from DCP-MEM-BR1X, and how do I supplement it?

Sodium bicarbonate is removed for CO2-independent, HEPES-bufferable use; phenol red is removed to reduce interference in fluorescence-based imaging and biosensor assays. Add HEPES (15-25 mM) for pH buffering if extended bench time outside a CO2 incubator is needed. L-Glutamine and sodium pyruvate are already present in this formulation - do not re-supplement these separately.

Q: Does this medium require a CO2 incubator?

No. This formulation is CO2-independent because sodium bicarbonate has been excluded. Supplement with HEPES (15-25 mM) for pH buffering if culturing outside a CO2 incubator for extended periods.

Q: Can DCP-MEM-BR1X be supplemented with serum, growth factors, or other components?

Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 micron low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use a 0.1 micron membrane. Do not use a 0.04 micron membrane for supplement filtration - it retains IgM, VLDL, and lipoprotein fractions that serum activity depends on. Because L-Glutamine and sodium pyruvate are already present, avoid re-adding these when supplementing.

Q: What endotoxin level should I expect, and how is it verified?

FluxMPS DCP-MEM-BR1X is released to meet less than 0.05 EU/mL by LAL assay (USP less than 85), tested per manufacturing batch rather than per unit. For primary cells, endotoxin can activate TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. Full CoA per batch covers appearance, pH (USP less than 791), osmolality (USP less than 785), sterility (USP less than 71), endotoxin (USP less than 85), mycoplasma-retentive filtration status, particulate count (USP less than 788 Method 1), and raw-material traceability. Request at support@diagnocine.com.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

L-Glutamine Calcium Magnesium Glucose (Low, 1.0 g/L) Sodium Pyruvate Sodium Bicarbonate Phenol Red

FluxMPS™ DCP-MEM-BR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA formulation with Earle's Salts, engineered for primary fibroblast, neuronal, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. NEAA is pre-loaded to reduce metabolic burden on primary cells, and the formulation omits sodium bicarbonate and phenol red for CO₂-independent, imaging-friendly culture.

Low Glucose (1.0 g/L, 1000 mg/L) — a physiological carbon source for primary cells sensitive to high-glucose stress
NEAA pre-loaded (5 amino acids) — reduces de novo synthesis burden and ammonia accumulation in primary cell culture
Sodium bicarbonate and phenol red both excluded — supports CO₂-independent, HEPES-bufferable, autofluorescence-conscious imaging workflows
Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
Endotoxin release specification < 0.05 EU/mL (USP <85> BET), per manufacturing batch
Manufactured under an ISO 13485:2016 quality management system with full lot traceability; final QA at Diagnocine, Totowa, NJ
pH 7.4 (USP <791>); osmolality 235 - 275 mOsm/kg H₂O (USP <785>)

DCP-MEM-BR1X | Cell Culture Media

Pack sizes: 500 mL, 1000 mL

UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Glucose1000 mg/L (1.0 g/L, Low Glucose)
L-Glutamine292 mg/L
Sodium Pyruvate110 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)235 - 275 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
Storage2-8°C, protected from light
Shelf Life12 months from date of manufacture, unopened
ShippingCold pack

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM carries mycoplasma risk, particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ is filtered to a 0.04 µm final cut-off while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Ultra-low particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) supports trace-metal and organic-carbon (TOC) control during manufacture.

shield

Batch-controlled endotoxin specification

< 0.05 EU/mL release specification (USP <85> BET), tested per manufacturing batch. Relevant to TLR4-mediated fibroblast activation, neuroinflammatory signaling, and epithelial barrier studies.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

1

0.1 µm Prefiltration I

Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; provides mycoplasma-retentive filtration (organisms typically 0.2–0.3 µm) beyond what a standard 0.22 µm filter retains.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEM-BR1X is processed through two dedicated prefilter/final-filter pairs reaching a 0.04 µm final cut-off, compared with a single 0.22 µm pass typical of conventional media.

4

Sequential filtration passes

0.04

µm Final pore size

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration at Stage 1 and Stage 3 (not tested per lot).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS™ DCP-MEM-BR1X Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable MEM Low Glucose + NEAA for organ-on-a-chip (OoC) and microphysiological systems (MPS) | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-BR1X

Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEM-BR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

A separate 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactor and robotic handling systems.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose provides a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip

Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip

Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts

Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS

Live-Cell Imaging

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy, TEER sensor, and biosensor applications on primary cell chips; phenol red is excluded from this formulation.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate \| [-] Sodium Bicarbonate, [-] Phenol Red
Appearance	Pale yellow-colored, clear solution
Glucose	1000 mg/L (1.0 g/L, Low Glucose)
NEAA	Present (5 amino acids)
pH USP <791>	7.4
Osmolality USP <785>	235 - 275 mOsm/kg H₂O
Total ingredients	35

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification)
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100) aseptic fill

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2-8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent; supplement with HEPES (15-25 mM) for pH buffering

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 35 ingredients verified per lot with CAS numbers where known.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30925-07-6	31.300
L-Glutamic acid	56-86-0	14.700
L-Glutamine	56-85-9	292.000
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate	69847-15-0	51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
OTHERS
i-Inositol	87-89-8	2.000
D-Glucose	50-99-7	1000.000
Sodium pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-MEM-BR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, supporting trace-metal and TOC control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration particle count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235 - 275 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-MEM-BR1X compares

FluxMPS™ DCP-MEM-BR1X vs. conventional 0.22 µm-filtered MEM Low Glucose + NEAA formulations.

Parameter	DCP-MEM-BR1X (FluxMPS™)	Conventional MEM Low Glucose (0.22 µm filtered)	Standard DMEM (0.22 µm)
Grade	Microfluidics Suitable	Not specified	Not specified
MEM + NEAA without sodium bicarbonate and phenol red — CO₂-independent, imaging-friendly primary cell base	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma-retentive filtration	check_circle Yes (0.1 µm stages)	cancel No	cancel No
NEAA included	check_circle Yes	Optional add-on	cancel No
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested	check_circle Yes (Method 1)	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle Yes (Microfluidics Suitable)	cancel Higher clogging risk	cancel Higher clogging risk
Custom formulation	check_circle Available on request	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-BR1X.

Is DCP-MEM-BR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-MEM-BR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration and an endotoxin release specification < 0.05 EU/mL is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

FluxMPS™ uses four sequential stages: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish, two dedicated prefilter/final-filter pairs reaching a 0.04 µm final cut-off versus a single 0.22 µm pass in conventional media.

Why are sodium bicarbonate and phenol red both excluded from DCP-MEM-BR1X, and how do I supplement it?expand_more

Sodium bicarbonate is removed for CO₂-independent, HEPES-bufferable use; phenol red is removed to reduce interference in fluorescence-based imaging and biosensor assays. Add HEPES (15-25 mM) for pH buffering if extended bench time outside a CO₂ incubator is needed. L-Glutamine and sodium pyruvate are already present in this formulation — do not re-supplement these separately.

Does this medium require a CO₂ incubator?expand_more

No. This formulation is CO₂-independent because sodium bicarbonate has been excluded. Supplement with HEPES (15-25 mM) for pH buffering if culturing outside a CO₂ incubator for extended periods.

Can DCP-MEM-BR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use a 0.1 µm membrane. Do not use a 0.04 µm membrane for supplement filtration — it retains IgM, VLDL, and lipoprotein fractions that serum activity depends on. Because L-Glutamine and sodium pyruvate are already present, avoid re-adding these when supplementing.

What endotoxin level should I expect, and how is it verified?expand_more

FluxMPS™ DCP-MEM-BR1X is released to meet < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch rather than per unit. For primary cells, endotoxin can activate TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per batch covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA in primary cell OoC applications.

Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Engineered for primary cell culture where standard media fails

Microchannel-safe purity

Primary cell–optimized formulation

Ultrapure-grade water

Batch-controlled endotoxin specification

NEAA reduces metabolic burden

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

Primary cell models and OoC applications

Automated Bioreactors & Robotics

Neuronal Chips & Brain-on-Chip

Epithelium-on-Chip

Primary Fibroblast & Stromal Models

Vascular Cell Culture

Organ-on-a-Chip & MPS

Low background for imaging

Analytical release specifications

Full composition (mg/L)

Manufacturing & compliance

ISO 13485:2016 Quality Management

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin — USP <85> BET

Particulate — USP <788> Method 1

Osmolality — USP <785>

Documentation & CoA

How DCP-MEM-BR1X compares

Frequently asked questions

Supporting literature

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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid