FluxMPS Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Sodium Bicarbonate Calcium Magnesium Low Glucose L-Glutamine Phenol Red Sodium Pyruvate

FluxMPS™ DCP-MEM-QPR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA formulation engineered for primary fibroblasts and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red.

Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose media
NEAA pre-loaded reduces de novo synthesis burden and ammonia accumulation in primary cell culture
Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — suited to sensitive primary and hematopoietic cell cultures
L-Glutamine, Sodium Pyruvate and Phenol Red are excluded — add fresh at time of use for full metabolic control and autofluorescence-free imaging baselines
Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ

DCP-MEM-QPR1X | Cell Culture Media Sizes: 500 mL, 1000 mL UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Media familyMEM Low Glucose + NEAA
Glucose1000 mg/L (1.0 g/L, Low Glucose)
NEAAPresent
Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] Sodium Bicarbonate
[-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
AppearancePale yellow-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)290–330 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes particulates, mycoplasma-sized organisms, and endotoxin that can alter primary cell phenotype. FluxMPS™ is built to reduce these risks while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon (TOC) content, formulated for sensitive primary cell applications.

shield

Low endotoxin release specification

< 0.05 EU/mL endotoxin release specification, near or below common TLR4 activation thresholds reported for primary cell models — relevant to fibroblast activation, neuroinflammatory signaling, and epithelial barrier studies.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — a repeated prefilter + final-filter pair, run twice — reaching a final 0.04 µm polish.

1

0.1 µm Prefiltration I

Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and organisms in the mycoplasma size range (0.2–0.3 µm) that a standard 0.22 µm filter does not.
3

0.1 µm Prefiltration II

A second, dedicated prefilter protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size across 4 filtration passes

Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: controlled via 0.1 µm mycoplasma-retentive filtration applied at every production stage (not tested per lot).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS DCP-MEM-QPR1X Minimum Essential Medium (MEM) Low Glucose NEAA w/o L-Glutamine Sodium Pyruvate Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Low Glucose + NEAA for organ-on-a-chip and microfluidic applications | Diagnocine — **Figure 1.** FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-QPR1X

Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEM-QPR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactor and robotic liquid-handling systems.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip

Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity studies in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip

Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts

Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline (0.04 µm final filtration) supports confocal, TEER-sensor, and biosensor applications on primary cell chips; this formulation is phenol red-free.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate \| [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
Appearance	Pale yellow-colored, clear solution
Glucose	1000 mg/L (1.0 g/L, Low Glucose)
NEAA	Present (Gly, Ala, Asn, Asp, Glu, Pro)
L-Glutamine	None added (excluded — add fresh at time of use)
Sodium Pyruvate	None added (excluded — add fresh at time of use)
Phenol Red	None added (excluded — phenol red-free)
pH USP <791>	7.4
Osmolality USP <785>	290–330 mOsm/kg H₂O
Total ingredients	34 components across 3 formulation groups

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ incubator required (sodium bicarbonate buffered)
Pack sizes	500 mL, 1000 mL

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 34 ingredients verified per lot with CAS numbers. NEAA is listed under AMINO ACIDS.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2200.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30925-07-6	31.300
L-Glutamic acid	56-86-0	14.700
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate	69847-15-0	51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
OTHERS
i-Inositol	87-89-8	2.000
D-Glucose	50-99-7	1000.000

Custom formulation: Contact support@diagnocine.com for DCP-MEM-QPR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, controlled trace-metal and TOC content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available for any DCP-MEM-QPR1X lot at support@diagnocine.com.

Product Comparison

How DCP-MEM-QPR1X compares

FluxMPS™ DCP-MEM-QPR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter	DCP-MEM-QPR1X (FluxMPS™)	Conventional MEM Low Glucose (0.22 µm filtered)	Standard DMEM (0.22 µm)
Grade	Microfluidics Suitable (0.04 µm)	Not applicable	Not applicable
MEM + NEAA without L-Glutamine, Pyruvate, and Phenol Red	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple-stage)	1	1
Mycoplasma-retentive filtration	check_circle Yes (0.1 µm stage)	cancel No	cancel No
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance (Method 1)	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatibility	check_circle Microfluidics Suitable	cancel Risk of clogging	cancel Risk of clogging
Custom formulation available	check_circle Yes	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-QPR1X.

Is DCP-MEM-QPR1X suitable for organ-on-a-chip (OoC) applications?expand_more

DCP-MEM-QPR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with 0.1 µm mycoplasma-retentive filtration is well suited to primary neuronal, epithelial, and fibroblast OoC platforms.

What makes the filtration superior to standard 0.22 µm filtered media?expand_more

Why are L-glutamine, sodium pyruvate, and phenol red all excluded from DCP-MEM-QPR1X?expand_more

Phenol red is removed for autofluorescence-free imaging; L-glutamine and sodium pyruvate are removed so users can supplement fresh at time of use, since both degrade or are consumed during storage and culture. Sodium bicarbonate provides standard CO₂ buffering. This design enables clean optical baselines with full metabolic control for live-cell confocal and TEER-monitored primary cell OoC platforms.

Does DCP-MEM-QPR1X require a CO₂ incubator?expand_more

Yes. This formulation contains 2200 mg/L sodium bicarbonate and is designed for use in a 5% CO₂ incubator to maintain pH near 7.4.

What role does NEAA play in MEM formulations?expand_more

NEAA are pre-loaded, providing non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that would otherwise synthesize these amino acids de novo from glutamine and glucose, improving viability in low-serum conditions and reducing ammonia accumulation.

Can DCP-MEM-QPR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not appropriate for serum and will strip out lipoproteins and clog quickly. Defined, protein-free additions may use a 0.1 µm filter. L-Glutamine and sodium pyruvate can be added fresh at time of use.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Every batch is tested before release and a Certificate of Analysis is available on request, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>, LAL, release specification < 0.05 EU/mL), and particulate count (USP <788> Method 1). Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA in primary cell OoC applications.

Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid