FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ DCP-DMEMH-PR1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO₂-variable or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides supplemental pH buffering alongside the retained sodium bicarbonate system. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red.

• High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and other aerobically active cells
• 25 mM HEPES (pKa 7.3 at 37°C) — supplemental pH buffering alongside the bicarbonate system for handling outside the incubator
• 0.04 µm final filtration — retains particulates and organisms in the mycoplasma size range (0.2–0.3 µm) for microfluidic channels below 100 µm
• Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
• Endotoxin batch release specification < 0.05 EU/mL (LAL, USP <85>)
• Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
• Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes fine particulates and organisms in the mycoplasma size range that clog microfluidic channels. Standard DMEM also relies solely on bicarbonate buffering, causing pH drift whenever chips are handled outside a CO₂ incubator. FluxMPS™ addresses both failure modes.

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge from premature fouling.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and organisms in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm cartridge — full redundancy against upstream bypass.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-PR1X combines high-glucose energy support with supplemental HEPES buffering — supporting platforms from open-top microfluidic chips to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

HEPES buffering supports pH stability during automated bioreactor perfusion outside continuous CO₂ control. An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for robotic liquid handlers where trace particulates cause valve failure.

• Reduced Particulate Load: Quadruple-stage 0.04 µm filtration reduces particulate carryover in bioreactor media lines
• pH-Stable Automated Delivery: HEPES helps maintain pH during robotic media exchanges outside a CO₂ incubator
• Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 32 ingredients verified per lot with CAS numbers for raw-material traceability. HEPES (25 mM = 5958 mg/L) and D-Glucose are listed under OTHERS.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-DMEMH-PR1X compares

FluxMPS™ DCP-DMEMH-PR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-PR1X DMEM High Glucose + 25mM HEPES.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j