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FluxMPS™ DMEM, No Glucose Family — 2X Liquid Cell Culture Media

Product#: DulbeccosModifiedEagleMedium(DMEM)NoGlucose2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
history_edu Built on Dulbecco & Freeman's 1959 Eagle Modification

FluxMPS™ DMEM, No Glucose [2X] Family — 2X Liquid Cell Culture Media

A 32-variant DMEM with no Glucose platform, supplied as a 2X (double-strength) liquid concentrate, built on the four-fold amino acid and vitamin enrichment Renato Dulbecco and G. Freeman introduced to Eagle's medium in 1959. Removing glucose creates a defined basal medium for studying cell metabolism under low- or no-glucose conditions; glucose, galactose, or another carbon source can be added at any level. Every variant is purified through FluxMPS™ quadruple-stage 0.04 micron filtration, engineered for organ-on-chip (OoC), tissue-on-chip (ToC), and lab-on-chip (LoC) microfluidic culture systems where particulate load and channel clogging are not an option.

  • 32 variants, each a 2X liquid concentrate: a full DMEM configuration portfolio including the customization of L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, HEPES (50 mM at 2X; 25 mM at 1X), and Phenol Red.
  • 2X (double-strength) format: dilute 1:1 to reach 1X working strength. Add glucose, galactose, or another carbon source at any level for glucose-deprivation, dose-response, glucose-uptake, insulin-signaling, cancer-metabolism, AMPK/autophagy, and ischemia models.
  • Glucose: none — a basal medium for metabolic-restriction studies relevant to cancer and stem cell biology.
  • Rich nutrient profile: Includes 15 amino acids — Eagle’s essential set plus glycine and L-serine — and 8 water-soluble vitamins at approximately 4x the Basal Medium Eagle (BME) level.
  • Flexible buffering options: sodium bicarbonate buffer system that helps maintain physiological pH in a 5–10% CO2 incubator, with 50 mM HEPES variants (25 mM at 1X working strength) also available.
  • FluxMPS™ quadruple-stage 0.04 micron filtration — finer than any ready-to-use cell culture media currently available.
  • Validated across DMEM-standard lines including HEK293/293T, NIH/3T3, COS-7, RAW 264.7, HeLa, and primary mouse embryonic cells.
  • Ships in 500 mL bottles. Stored at 2–8 °C away from bright light. Custom concentration, pH, glucose concentration, salts, HEPES level, and added supplements available on request — please contact support@diagnocine.com.
FAMILY: DMEM · 32 SKU VARIANTS Cell Culture Media
FluxMPS™ DMEM, No Glucose — 2X Liquid
  • Concentration2X (double strength)
  • Working strength1X after 1:1 dilution
  • GlucoseNone
  • L-Glutamine / Sodium PyruvateConfigurable (+/-)
  • Sodium Bicarbonate / HEPES (50 mM at 2X)Configurable (+/-)
  • Phenol RedConfigurable (+/-)
  • Salt baseNaCl, KCl, CaCl2, MgSO4, NaH2PO4, +Fe(NO3)3
  • BufferingCO2-dependent, Bicarbonate, and/or HEPES (25 mM at 1X)
  • Sizes500 mL
  • Storage2–8°C, away from bright light
RUO DULBECCO & FREEMAN 1959 FOUNDATIONAL 2X CONCENTRATE CUSTOMIZABLE
Product Selector

Select Your DMEM, Glucose-Free [2X] Configuration

Check the supplement(s) you need below — every matching 2X variant is highlighted immediately, and pressing Search also scrolls to the first match. Each row links to its product page either by clicking the catalog number or the View button.

DMEM, No Glucose [2X] Family — At-a-glance supplement matrix (32 variants)
Select the supplement(s) of interest — matching rows are highlighted immediately; Search also scrolls to the first match. Click any catalog number or View button to open the product page.
Filter by included supplements
 
Name Cat No. L-Glutamine Sodium Pyruvate Sodium Bicarbonate HEPES (50mM) Phenol Red Product Page
DMEM, 2X w/o Glucose DCP-DMEM-G2X check check check remove check Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine DCP-DMEM-GQ2X remove check check remove check Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Pyruvate DCP-DMEM-GP2X check remove check remove check Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Bicarbonate DCP-DMEM-GB2X check check remove remove check Viewarrow_forward
DMEM, 2X w/o Glucose, Phenol Red DCP-DMEM-GR2X check check check remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Pyruvate DCP-DMEM-GQP2X remove remove check remove check Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Bicarbonate DCP-DMEM-GQB2X remove check remove remove check Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Phenol Red DCP-DMEM-GQR2X remove check check remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate DCP-DMEM-GPB2X check remove remove remove check Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Pyruvate, Phenol Red DCP-DMEM-GPR2X check remove check remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Bicarbonate, Phenol Red DCP-DMEM-GBR2X check check remove remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate DCP-DMEM-GQPB2X remove remove remove remove check Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Pyruvate, Phenol Red DCP-DMEM-GQPR2X remove remove check remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Bicarbonate, Phenol Red DCP-DMEM-GQBR2X remove check remove remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red DCP-DMEM-GPBR2X check remove remove remove remove Viewarrow_forward
DMEM, 2X w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red DCP-DMEM-GQPBR2X remove remove remove remove remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose DCP-DMEMH-G2X check check check check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine DCP-DMEMH-GQ2X remove check check check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Pyruvate DCP-DMEMH-GP2X check remove check check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Bicarbonate DCP-DMEMH-GB2X check check remove check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Phenol Red DCP-DMEMH-GR2X check check check check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate DCP-DMEMH-GQP2X remove remove check check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate DCP-DMEMH-GQB2X remove check remove check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Phenol Red DCP-DMEMH-GQR2X remove check check check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate DCP-DMEMH-GPB2X check remove remove check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Pyruvate, Phenol Red DCP-DMEMH-GPR2X check remove check check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red DCP-DMEMH-GBR2X check check remove check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate DCP-DMEMH-GQPB2X remove remove remove check check Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Phenol Red DCP-DMEMH-GQPR2X remove remove check check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate, Phenol Red DCP-DMEMH-GQBR2X remove check remove check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red DCP-DMEMH-GPBR2X check remove remove check remove Viewarrow_forward
DMEM, 2X + HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red DCP-DMEMH-GQPBR2X remove remove remove check remove Viewarrow_forward
check Included remove Not included All variants: 2X concentration (dilute 1:1 for 1X) · Glucose-free · quadruple-stage 0.04 µm filtration · 500 mL
Customization available: All variants above use the standard 2X concentration (dilute 1:1 for 1X), Glucose-Free, and (where present) 50 mM HEPES at 2X (25 mM at 1X). Other concentrations, glucose levels, buffer levels, chemical additions, compounds, proteins, supplements, pH adjustments, or further modifications are available on request — contact support@diagnocine.com.
About the Medium

What is DMEM, and why a No-Glucose build?

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modifications of Eagle's medium. It is a modification of Basal Medium Eagle (BME) that contains fourfold concentrations of amino acids and vitamins, and additionally includes glycine, serine, and ferric nitrate. The original formulation contains 1 g/L of glucose and was originally used to culture embryonic mouse cells. DMEM is an enriched derivative of BME and a default choice for high-density and fast-growing adherent cultures, well suited to metabolically demanding applications.

In this family, glucose is removed to provide a basal medium for studying the metabolic activity and response of cells under low or no glucose conditions. This formulation lets researchers investigate how cells adapt and function in environments with limited glucose availability — particularly relevant for research areas such as cancer and stem cell biology.

Origins and Development

DMEM traces directly to Harry Eagle's foundational work at the National Institutes of Health, where his 1955 studies defined the minimal nutritional requirements of HeLa and L (L929) cells and led to the development of Basal Medium Eagle (BME). Eagle later noted that BME emerged from systematic nutritional studies rather than from a planned attempt to design a culture medium. In 1959, Eagle expanded BME into Minimum Essential Medium (MEM) by increasing amino acid concentrations to extend culture maintenance and broaden the range of supported cell lines.

That same year, Renato Dulbecco and G. Freeman introduced their own modification of Eagle's medium in a footnote in their Virology paper on plaque production by the polyoma virus (Virology 8: 396–397, 1959). The modification — now universally known as Dulbecco's Modified Eagle Medium (DMEM, or DME) — had substantially higher concentrations of amino acids and vitamins than BME and was used to support the culture of mouse embryonic cells in monolayers needed for quantitative virus plaque assays. As with Eagle's original work, the medium emerged as a practical tool in service of a virological objective rather than as a standalone engineering goal, and it likewise depended on serum supplementation to support proliferation.

In the decade that followed, the formulation was adapted and commercialized in several variants. Higher-glucose versions (4.5 g/L) were developed to support rapidly proliferating and transformed cell lines, and DMEM was extended to primary cultures of mouse and chicken cells, as well as a broad spectrum of normal and transformed cell types. Surveys of commercial media from this period (Morton, 1970) document how DMEM diversified into the family of glucose-, pyruvate-, glutamine-, and buffer-variant media still in use today.

Note on parent formulation: Multiple authoritative sources — including the Cantor 2019 Trends in Cell Biology review, Sigma-Aldrich, and Corning — classify DMEM as a derivative of BME. Some commercial literature describes the fourfold enrichment relative to "Eagle's Minimal Essential Medium"; the Dulbecco footnote itself refers simply to "Eagle's medium." The scientific consensus, anchored by primary review literature, identifies BME as the direct parent.

Why a 2X (Double-Strength) Format

Every member of this family is supplied at 2X: each formulated component is present at twice its working level, so that diluting the medium 1:1 brings every component to standard 1X strength. Because the base carries no glucose, the second half of the volume is where the researcher sets the carbon source — the product pages state that glucose, galactose, or another carbon source can be added at any level, for glucose-deprivation, dose-response, glucose-uptake, insulin-signaling, cancer-metabolism, AMPK/autophagy, and ischemia models. HEPES, in the HEPES variants, is 50 mM at 2X and 25 mM at 1X. Components a variant name lists as "w/o" are left out so they can be added fresh at their 1X levels.

Lineage

The DMEM Family Tree

DMEM sits in the middle of a lineage that begins with Eagle and continues into several enriched descendants:

  • BME (1955, Eagle) — the parent formulation; a basal amino acid and vitamin mix, including glutamine, biotin, and baseline inorganic salts.
  • MEM (1959, Eagle) — a BME derivative with increased amino acid concentrations; biotin was omitted after Eagle found it dispensable.
  • DMEM (1959, Dulbecco & Freeman) — a further-enriched BME modification (approximately 4x amino acids and vitamins vs. BME) with 15 amino acids (adding glycine and L-serine to the Eagle essential set) and added ferric nitrate, originally for mouse embryonic cells and viral plaque assays.
  • DMEM/F-12 — a 1:1 mixture of high-glucose DMEM and Ham's F-12, combining DMEM's rich amino acid/vitamin profile with F-12's trace elements and lipids for lower-serum and serum-free work.
  • IMDM (Iscove's Modified Dulbecco's Medium, 1978; Iscove & Melchers) — an enriched DMEM derivative adding selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate in place of ferric nitrate; designed for high-density, rapidly proliferating cultures such as lymphocytes and hematopoietic cells.

In parallel, the broader Eagle lineage also gave rise to RPMI 1640, developed by Moore, Gerner, and Franklin at Roswell Park Memorial Institute (1966; published in JAMA 199: 519–524, 1967). RPMI 1640's direct parent is McCoy's 5A Modified Medium, which was itself based in part on BME — illustrating how a single nutritional framework branched into the modern catalog of classical media.

Composition

DMEM, No Glucose — Component Summary

2X scale note: The values in the table below are given at the 1X reference (working) scale. The 2X concentrate contains each formulated component at twice that level and reaches these values after 1:1 dilution; the only 2X value stated on the product pages is HEPES (50 mM at 2X).

Categorical composition summary as documented in the source formulation record. Per-variant Certificate of Analysis (CoA) requests can be directed to support@diagnocine.com.

Component Summary
Concentration 2X liquid concentrate; dilute 1:1 to reach 1X working strength.
Amino acids 15 amino acids — Eagle's essential set plus glycine and L-serine; concentrations approximately 4x BME baseline.
Vitamins 8 water-soluble vitamins at ~4× BME baseline: choline chloride, folic acid, myo-inositol, niacinamide, D-calcium pantothenate, pyridoxine HCl (dominant Gibco/Thermo Fisher formulation; some suppliers substitute pyridoxal HCl), riboflavin, thiamine HCl. Biotin and vitamin B12 are absent.
Inorganic salts NaCl, KCl, CaCl2, MgSO4, NaH2PO4, NaHCO3, plus ferric nitrate [Fe(NO3)3·9H2O] as a distinctive trace-iron source (0.1 mg/L)
Glucose None (this family is glucose-free); add glucose, galactose, or another carbon source at any level.
Sodium pyruvate Approximately 110 mg/L (1 mM) at 1X in variants that include it; configurable per the supplement matrix above.
Serum requirement Not a complete medium; typically 10% FBS (5–20% depending on cell line)
pH buffering Sodium bicarbonate buffer system (approximately 3.7 g/L NaHCO3) maintaining physiological pH, necessitating incubation in a 5-10% CO2 environment, or HEPES in the HEPES-supplemented variants (50 mM at 2X; 25 mM at 1X); phenol red as pH indicator where included.
pH / osmolality 7.4 ± 0.04; typically 320–370 mOsm/kg depending on formulation and manufacturer
Vitamin B6 note: The dominant commercial standard (Gibco/Thermo Fisher DMEM) uses pyridoxine HCl. Some other suppliers specify pyridoxal HCl instead. Both are valid B6 vitamer forms. DiagnoCine Precision applies pyridoxal HCl unless otherwise mentioned in the COA, respectively.
Comparison

Media Lineage Comparison — BME vs MEM vs DMEM

Feature BME MEM DMEM (FluxMPS™ 2X family, at 1X)
Amino acid level Baseline — minimal ~2× BME ~4× BME
Vitamin level Baseline, includes Biotin Often lacks Biotin 4× BME
Glucose 1.0 g/L 1.0 g/L Glucose-Free
Added iron source None None Ferric nitrate, 0.1 mg/L
Serum supplement 5–10% recommended 5–10% recommended 10–20% typical
pH buffering Bicarbonate, CO2-dependent Bicarbonate, CO2-dependent Bicarbonate, 5-10% CO2 (or HEPES)
Developer & year Harry Eagle, 1955 Harry Eagle, 1959 Dulbecco & Freeman, 1959

Values in the comparison above refer to 1X working strength.

DMEM, No Glucose: 1X vs. 2X

Feature DMEM, No Glucose, 1X DMEM, No Glucose, 2X
Supplied concentration 1X (ready to use) 2X (double strength)
Preparation before use None Dilute 1:1 for 1X working strength
Component concentrations as supplied Standard DMEM, No Glucose Twice the 1X level, before 1:1 dilution
Glucose None None — add glucose, galactose, or another carbon source at any level
HEPES (HEPES variants) 25 mM 50 mM at 2X; 25 mM at 1X after dilution
Sodium pyruvate (where included) Approximately 110 mg/L (1 mM) Approximately 110 mg/L (1 mM) at 1X after dilution
Catalog variants 32 (all L-Glutamine / Sodium Pyruvate / Sodium Bicarbonate / HEPES / Phenol Red combinations) 32 (the same 32 combinations)
Catalog number suffix ...1X (e.g., DCP-DMEM-G1X) ...2X (e.g., DCP-DMEM-G2X)
Typical uses Metabolic studies under low or no glucose (cancer and stem cell biology); DMEM-standard lines Glucose-deprivation, dose-response, glucose-uptake, insulin-signaling, cancer-metabolism, AMPK/autophagy, ischemia models
Filtration Quadruple-stage, 0.04 micron final Quadruple-stage, 0.04 micron final
Sizes 500 mL, 1000 mL 500 mL
Storage 2-8°C, away from bright light 2-8°C, away from bright light
Why FluxMPS™

Media engineered for the architecture of the future

FluxMPS™ was designed from the ground up for microphysiological systems, where the medium itself is part of the instrument.

filter_alt

Purified to 0.04 microns

Finer than any ready-to-use cell culture media currently available, removing particulates and protein aggregates before they reach your chip.

water

Engineered for laminar flow

Every component is optimized for consistent flow performance — zero-clogging is the baseline specification, not a feature.

visibility

Optical clarity for imaging

Clean matrices support real-time imaging and integrated biosensing in Organ-on-Chip readouts.

developer_board

Built for OoC / ToC / LoC

Designed specifically for Organ-on-Chip, Tissue-on-Chip, and Lab-on-Chip microfluidic platforms.

schedule

Stable over long perfusion

Consistent formulation stability across automated perfusion studies running continuously for weeks.

verified

Regulatory foundation

Formulated to support FDA-recognized physiological modeling standards for drug discovery, toxicology, and translational research.

Purity

Quadruple-Stage Filtration System

Every FluxMPS™ DMEM, No Glucose [2X] variant passes through the same four-stage architecture before reaching your chip — two 0.1 µm pre-filtration passes and two 0.04 µm final filtration passes, each final filter preceded by its own prefilter.

  • 01

    Pre-Filtration Stage 1 0.1 µm

    Initial coarse particulate removal.

  • 02

    Final Filtration Stage 1 0.04 µm

    Fine filtration below standard 0.22 micron practice.

  • 03

    Pre-Filtration Stage 2 0.1 µm

    Secondary particulate and aggregate reduction.

  • 04

    Final Filtration Stage 2 0.04 µm

    Final polish for microfluidic-grade clarity.

Engineered for Flow, Not Just Growth

Every component is optimized for consistent, laminar flow performance across complex micro-channel geometries, capillary-bed and vascular simulations, and long-term automated perfusion studies running continuously for weeks.

0.04
micron final filtration
4
Total filtration stages
FluxMPS™ DMEM, No Glucose [2X] Family quadruple-stage filtration system diagram ? alternating 0.1 micron prefilters and 0.04 micron final filters, engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and lab-on-a-chip microfluidic cell culture media applications by Diagnocine.
Validated Cell Lines

Cell lines routinely cultured in DMEM

DMEM is the standard or recommended medium across a wide range of cell types. Primary mouse and chicken cultures were among the early extensions of DMEM beyond its original mouse-embryo use.

biotech

Mouse embryonic cells / MEFs

Primary mouse embryonic cells and mouse embryonic fibroblasts — the original target of Dulbecco & Freeman's formulation.

grid_view

NIH/3T3 fibroblasts

A classic immortalized adherent line routinely maintained in DMEM.

vaccines

HEK293 / HEK293T

The workhorse lines for transient transfection and recombinant protein and viral-vector production, typically in high-glucose DMEM.

science

COS-7

SV40-transformed African green monkey kidney cells used for transient expression.

coronavirus

RAW 264.7 macrophages

A mouse macrophage line maintained in DMEM.

spoke

HeLa & transformed epithelia

HeLa and other transformed epithelial lines are broadly supported in DMEM as well as in Eagle-family media.

Scientific Applications

Where DMEM, Glucose-Free [2X] fits

cyclone

Glucose-restriction & metabolic studies

A defined basal medium for studying metabolic activity and cell responses under low or no glucose — particularly relevant to cancer and stem cell biology.

hub

Transfection & viral-vector production

High-glucose DMEM is the default for HEK293/293T workflows — transient transfection, recombinant protein expression, and lentivirus/AAV packaging; the glucose-free base supports controlled metabolic variants of these workflows.

grid_on

General adherent & high-density culture

Nutrient levels well above the minimal Eagle baseline support fast proliferation of fibroblasts and many transformed adherent lines.

balance

Physiologic media comparison

DMEM is the canonical "traditional" medium whose nutrient levels diverge sharply from human plasma, and serves as the principal comparator (alongside RPMI 1640) in the physiologic-media literature where HPLM and Plasmax are benchmarked against it (Cantor 2019).

FAQ

Frequently asked questions

The family includes 32 glucose-free 2X liquid configurations across a standard bicarbonate-buffered DMEM series (DCP-DMEM prefix) and a 50mM HEPES series (DCP-DMEMH prefix; 25 mM at 1X). Each variant differs by which supplements are included or omitted: L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, HEPES, and Phenol Red. Use the interactive supplement filter above the matrix — check the supplements you need and every matching variant row is highlighted so you can click straight through to its product page.
Dilute the 2X medium 1:1. Every formulated component falls to half its 2X level; HEPES (in HEPES variants) goes from 50 mM to 25 mM. Because the base is glucose-free, the second half of the volume is where you set the carbon source: the product pages state that glucose, galactose, or another carbon source can be added at any level. Components a variant is supplied without can be added fresh at their 1X levels, and salts, buffer, or serum in the second solution should be accounted for in the final mixture.
Yes. FluxMPS™ media are engineered from the ground up for Organ-on-Chip (OoC), Tissue-on-Chip (ToC), and Lab-on-Chip (LoC) platforms, where the medium itself is part of the instrument. Purification to 0.04 microns removes the microscopic particulates and protein aggregates that block micro-channels and disrupt laminar flow, supporting stable shear stress and long-term automated perfusion.
DMEM is not a complete medium and depends on serum supplementation to support proliferation. Typical use is about 10% FBS, with 5–20% depending on the cell line. As Dulbecco and Freeman's original formulation likewise relied on serum, DMEM should be supplemented before use.
Removing glucose provides a basal medium for studying the metabolic activity and response of cells under low or no glucose conditions. This allows researchers to investigate how cells adapt and function in environments with limited glucose availability, which is particularly relevant for research areas like cancer and stem cell biology. The 2X product pages list glucose-deprivation, dose-response, glucose-uptake, insulin-signaling, cancer-metabolism, AMPK/autophagy, and ischemia models.
DMEM is bicarbonate-buffered (sodium bicarbonate) and therefore CO2-dependent, typically requiring a 5–10% CO2 atmosphere to maintain physiological pH near 7.2. The DCP-DMEMH series adds HEPES (50 mM at 2X; 25 mM at 1X), a zwitterionic buffer that helps stabilize pH during handling outside the incubator. Select a HEPES variant for open-system or microfluidic handling where CO2 control is intermittent.
Every 2X variant is supplied as a 2X liquid in 500 mL. Store at 2–8°C, away from bright light.
Yes. The standard configuration for this family is 2X concentration, No Glucose, and 50 mM HEPES at 2X (25 mM at 1X) where applicable. Other concentrations, additions of chemicals, compounds, proteins, or supplements, a different pH, and further modifications are available on request. Contact support@diagnocine.com.
References

Verified Bibliography

  1. Dulbecco, R. & Freeman, G. (1959). Plaque production by the polyoma virus. Virology, 8(3), 396–397. PMID: 13669362
  2. Smith, J.D., Freeman, G., Vogt, M. & Dulbecco, R. (1960). The nucleic acid of polyoma virus. Virology, 12(2), 185–196.
  3. Eagle, H. (1955). Nutrition Needs of Mammalian Cells in Tissue Culture. Science, 122(3168), 501–504. PMID: 13255879
  4. Eagle, H. (1959). Amino Acid Metabolism in Mammalian Cell Cultures. Science, 130(3373), 432–437. PMID: 13658943
  5. Morton, H.J. (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro, 6(2), 89–108. PMID: 5523183
  6. Iscove, N.N. & Melchers, F. (1978). Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J. Exp. Med., 147(3), 923–933. PMID: 305462
  7. Moore, G.E., Gerner, R.E. & Franklin, H.A. (1967). Culture of normal human leukocytes. JAMA, 199(8), 519–524.
  8. Cantor, J.R. (2019). The Rise of Physiologic Media. Trends in Cell Biology, 29(11), 854–861. PMC7001851
  9. Yao, T. & Asayama, Y. (2017). Animal-cell culture media: History, characteristics, and current issues. Reproductive Medicine and Biology, 16(2), 99–117. PMC5661806
FluxMPS™ Platform

FluxMPS™ — Precision Cell Culture Media for Microphysiological Systems

Built for the architecture of the future. Not the flask of the past.

Traditional cell culture media were formulated for static well plates and flasks — environments that tolerate impurities, precipitates, and particle loads that would immediately compromise a microfluidic system. FluxMPS™ was designed from the ground up for Organ-on-Chip (OoC), Tissue-on-Chip (ToC), and Lab-on-Chip (LoC) platforms, where the medium itself is part of the instrument.

Purity That Protects Your Platform

FluxMPS™ is purified to 0.04 microns — finer than any ready-to-use cell culture media currently available. At this level, the microscopic particulates and protein aggregates that silently block micro-channels, disrupt laminar flow, and generate false biological signals are eliminated before the media ever reaches your chip.

The result: your platform stays operational, your data stays clean, and your biology drives the result — not your media.

Engineered for Flow, Not Just Growth

The name FluxMPS™ reflects its core design principle. Every component is optimized for consistent, laminar flow performance across:

  • Complex micro-channel geometries
  • Capillary-bed and vascular simulations
  • Long-term automated perfusion studies running continuously for weeks

Zero-clogging performance is not a feature — it is the baseline specification.

Applications & Performance

Application What FluxMPS™ Delivers
Microfluidics Stable shear stress; no channel blockage
Metabolic Tracing Ultra-pure matrix with no contaminant interference
Long-term Perfusion Consistent formulation stability over weeks of continuous flow
Organ-on-Chip Optical clarity for real-time imaging and integrated biosensing

Regulatory Foundation

FluxMPS™ is formulated to support FDA-recognized physiological modeling standards, providing a validated, reproducible media foundation for drug discovery, toxicology screening, and translational research. When your downstream data needs to stand up to regulatory scrutiny, your upstream media cannot be an afterthought.

The Bottom Line

Microfluidic platforms are precision instruments. They require precision inputs.

FluxMPS™ is the only ready-to-use cell culture medium engineered specifically to meet that standard — protecting your chip, your cells, and your science.

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