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FluxMPS™ Dulbecco's Modified Eagle Medium(DMEM), Low Glucose, 25mM HEPES w/o Phenol Red: 1X Liquid

Product#: DCP-DMEMLH-R1X
$71.50
DCP-DMEMLH-R1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium(DMEM), Low Glucose, 25mM HEPES w/o Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains 25mM HEPES Contains Calcium Contains Magnesium Contains Low Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-DMEMLH-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM Low Glucose formulation with 25 mM HEPES and no phenol red, engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. The 44 mM sodium bicarbonate base is supplemented with HEPES for extended pH stability during atmospheric handling, while phenol red removal reduces optical background from that component for imaging and biosensor applications.

  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final pore size
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Low Glucose formulation (1 g/L D-Glucose) with L-Glutamine (584 mg/L) and Sodium Pyruvate (110 mg/L) for defined carbon-source control
  • 25 mM HEPES (pKa 7.3 at 37°C) supplements the 44 mM sodium bicarbonate buffer system for extended pH stability during atmospheric handling and open-well procedures
  • Phenol red–free formulation reduces optical background from that component for confocal microscopy and biosensor applications
  • Manufactured under an ISO 13485:2016 quality management system, with per-lot Certificate of Analysis
  • Custom pH, salts, glucose concentration, and nutrient adjustments available on request
SKU: DCP-DMEMLH-R1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium(DMEM), Low Glucose, 25mM HEPES w/o Phenol Red: 1X Liquid
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose (Low), [+] Sodium Pyruvate | [-] Phenol Red
  • AppearanceColorless to pale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • CO2 Requirement10% CO2 recommended (bicarbonate-buffered); HEPES extends atmospheric stability
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ mitigates these failure modes with a four-stage 0.1/0.04 µm filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion across chip geometries.

target

Total metabolic control

Defined Low Glucose, L-Glutamine, and sodium pyruvate levels give researchers precise control over carbon-source and nitrogen inputs for metabolic studies.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) feed-water with tight trace-metal and organic-carbon control supports sensitive electrophysiology and biosensor work.

visibility

Low background for imaging

Quadruple-stage filtration provides an ultra-low particulate baseline for confocal microscopy and biosensor applications; phenol red is excluded from this formulation to remove that specific optical interference.

science

Rich, stable nutrient profile

33 verified ingredients per lot; micro-batch production with full raw-material traceability.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two paired prefilter + final-filter cycles — reach a final 0.04 µm polish, providing sub-mycoplasma-scale particulate control for microfluidic channels below 100 µm.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates, including mycoplasma-scale material (0.2–0.3 µm), that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-DMEMLH-R1X runs a paired 0.1 µm / 0.04 µm filtration train twice in series, reaching a 0.04 µm final pore size versus the 0.22 µm single-pass filtration typical of conventional media.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through the 0.1 µm and 0.04 µm mycoplasma-retentive filtration stages above (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMLH-R1X Dulbecco's Modified Eagle Medium (DMEM) Low Glucose 25mM HEPES w/o Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II - Microfluidics Suitable cell culture media | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for microfluidic and organ-on-a-chip applications.
© Diagnocine® — DCP-DMEMLH-R1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMLH-R1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. The supplemental 25 mM HEPES buffer extends pH stability for open-well microfluidic handling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers — see the Grade note above.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
  • Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-clean 0.04 µm–filtered media reduces microchannel clogging risk in multi-organ chip architectures.

OoCToCBoCLoCMPS
Bioproduction

CHO & Mammalian Cell Culture

Suited to CHO, cancer cell lines, and primary cells requiring a low-glucose, HEPES-buffered background.

CHOMCF-7HeLaHEK293
Stem Cell Biology

iPSC-Derived Models

Ultra-low endotoxin (< 0.05 EU/mL release specification) and multi-stage mycoplasma-retentive filtration support sensitive iPSC protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Low-particulate, endotoxin-controlled media supports HUVEC monolayer integrity and TEER monitoring.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined Low Glucose, L-Glutamine, and sodium pyruvate levels support 13C isotope tracing and NMR-based metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate background supports confocal imaging and biosensor platforms; phenol red is excluded to remove that specific interference source.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose (Low), [+] Sodium Pyruvate | [-] Phenol Red
Appearance Colorless to pale yellow, clear solution
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Glucose 1000 mg/L (Low Glucose)
L-Glutamine 584 mg/L
Sodium Pyruvate 110 mg/L
HEPES 5958 mg/L (25 mM), pKa 7.3 at 37°C
Phenol Red None / not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (per manufacturing batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1/0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 10% CO2 recommended (44 mM sodium bicarbonate buffering system); supplemental 25 mM HEPES extends pH stability for atmospheric handling but does not eliminate the need for CO2-supplemented incubation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Total: 33 components across 4 categories, verified per lot with CAS numbers for raw-material traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
D-Glucose 50-99-7 1000.000
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 7.200
Custom formulation: Contact support@diagnocine.com for DCP-DMEMLH-R1X custom specifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water with tight trace-metal and organic-carbon control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMLH-R1X compares

FluxMPS™ DCP-DMEMLH-R1X vs. conventional 0.22 µm–filtered DMEM Low Glucose + HEPES formulations.

Parameter DCP-DMEMLH-R1X (FluxMPS™) Conventional DMEM Low Glucose + HEPES
(0.22 µm filtered)		 Standard Alt.
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
HEPES-buffered, phenol red–free formulation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMLH-R1X.

Yes. DCP-DMEMLH-R1X is a Microfluidics Suitable medium processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, providing an ultra-low particulate background for MPS, OoC, tissue-on-a-chip (ToC), body-on-a-chip (BoC), and lab-on-a-chip (LoC) platforms.
FluxMPS™ runs a paired 0.1 µm prefilter / 0.04 µm final-filter train twice in series — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, 0.04 µm Final filtration II — reaching a 0.04 µm final pore size versus the single-pass 0.22 µm filtration typical of conventional media.
Phenol red is a pH indicator dye and a weak estrogen-receptor agonist; it is not a required nutrient, so no supplementation is needed to compensate for its absence. Removing it reduces optical background from that specific component for confocal microscopy, TEER sensors, and biosensor arrays. The 25 mM HEPES buffer and 44 mM sodium bicarbonate system are unaffected and provide combined pH stability.
Yes, 10% CO2 is recommended: the formulation retains its full 44 mM sodium bicarbonate buffering system. The supplemental 25 mM HEPES (pKa 7.3 at 37°C) extends pH stability during atmospheric handling and open-well procedures outside the incubator, but it does not eliminate the need for CO2-supplemented incubation for routine culture.
Yes. FBS, serum-free supplements, growth factors, antibiotics, or custom nutrients may be added as required. When adding serum-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane to maintain sterility without stripping proteins; do not use a 0.04 µm filter for serum additions. Contact support@diagnocine.com for custom co-formulation.
Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) prior to release and must meet the release specification of < 0.05 EU/mL. See the Product Comparison table for other suppliers' published endotoxin specifications.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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