FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1:1 DMEM/F-12 base supplemented with trace elements. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final cut-off
• DMEM/F-12 (1:1) base with Trace Elements; bicarbonate-free formulation (no HEPES added) — pH 7.4 (USP <791>)
• Glucose 3151 mg/L (3.151 g/L), L-Glutamine 365 mg/L, Sodium Pyruvate 110 mg/L, Phenol Red 8.63 mg/L
• Endotoxin release specification < 0.05 EU/mL (USP <85> BET), tested per manufacturing batch prior to release
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under strict trace-metal and TOC control
• Manufactured under an ISO 13485:2016 quality management system; final packaging & QC at Diagnocine, Totowa, NJ
• Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and variable endotoxin levels that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes at the source.

Quadruple-stage filtration system

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence reaching 0.04 µm — substantially reducing mycoplasma-sized particulates and subvisible particles that 0.22 µm filtration cannot address.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge downstream.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and microaggregates, including mycoplasma-sized particles (0.2–0.3 µm), that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm cartridge and providing filtration-train redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 laminar-flow workstation.

Designed for next-generation cell culture platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid is intended for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

• Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
• Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
• Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Lot-release quality parameters

Every production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery below before shipment.

Full composition (mg/L)

Complete formulation across four category groups (Inorganic Salts, Amino Acids, Vitamins, Others), presented in three navigable tabs below, comprising 57 total components, reproduced from manufacturer specification. Custom compositions available on request.

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

How DCP-DM12-B1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham’s F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175