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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Bicarbonate and Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Bicarbonate and Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEM-BR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate, [-] Phenol Red.
- High Glucose (4.5 g/L) — supports energy-demanding cell types: primary neurons, cardiomyocytes, cancer lines
- Quadruple-stage filtration system: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish)
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate, [-] Phenol Red
- Bicarbonate-free, phenol red-free formulation; CO₂-independent — pair with HEPES or an organic buffer for pH control
- Manufactured under an ISO 13485:2016 quality management system; final QA and testing at Diagnocine, Totowa, NJ, USA
- Custom pH, salts, glucose concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Calcium, [+] Magnesium, [-] Sodium Bicarbonate, [-] Phenol Red
- AppearancePale yellow-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)250–290 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and cellular debris that clog microfluidic channels and interfere with sensor signals. FluxMPS™ High Glucose addresses these failure modes while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in narrow chip geometries, including sub-100 µm channels.
High-energy cell support
4.5 g/L glucose (4× vs. low-glucose DMEM) sustains primary neurons, cardiomyocytes, cancer lines, and other aerobically active cells in prolonged perfusion.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with tightly controlled trace-metal and total organic carbon (TOC) content, minimizing background contamination in sensitive assays.
Low background for imaging
Ultra-low particulate content from 0.04 µm final filtration reduces optical scatter for confocal and biosensor platforms. This formulation does not contain phenol red.
Rich, stable nutrient profile
31 ingredients verified per lot; 4× BME amino acid/vitamin concentration; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish, run as two dedicated prefilter + final-filter pairs, deliver sub-mycoplasma particulate control unavailable from conventional single-stage 0.22 µm filtered media.
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1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates, cell debris, and protein aggregates; retains organisms in the mycoplasma size range (0.2–0.3 µm) and protects the first 0.04 µm cartridge from early fouling.
-
2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II — Redundant Barrier
Second dedicated prefilter, providing full-train redundancy and protecting the second 0.04 µm cartridge.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-DMEM-BR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEM-BR1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive cell types and aerobically active cultures.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and optical sensors
- Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS
Ultra-clean 0.04 µm–filtered media helps prevent microchannel clogging in complex multi-organ chip architectures.
Warburg Effect & Cancer Models
High glucose (4.5 g/L) supports aerobic glycolysis phenotyping in cancer cell lines requiring elevated energy substrates.
Primary Neurons & Brain-on-Chip
High glucose supports the metabolic demands of primary neurons and iPSC-derived neuronal networks in microfluidic perfusion.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM is widely used for iPSC-derived cardiomyocyte maturation and heart-on-chip functional assays.
Metabolic Flux Analysis
Bicarbonate-free, phenol red-free, defined high-glucose formulation suited to ¹³C isotope tracing, Agilent Seahorse XF assays, and glycolysis/OXPHOS profiling.
Microscopy & Optical Sensing
Ultra-low particulate content and a phenol red-free formulation reduce background scatter for confocal microscopy and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate, [-] Phenol Red |
| Appearance | Pale yellow-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 250–290 mOsm/kg H2O |
| Total ingredients | 31 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | CO2-independent; use HEPES or an organic buffer for pH control |
| Available pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 31 ingredients verified per lot with CAS numbers for full raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity, ASTM D1193 Type 1 water, with tightly controlled trace-metal and total organic carbon (TOC) content.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL, controlled per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 250–290 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEM-BR1X compares
FluxMPS™ DCP-DMEM-BR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEM-BR1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard Alt. DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Bicarbonate-free, phenol red-free High Glucose formulation — CO2-independent pH control | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed September 2, 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEM-BR1X DMEM High Glucose.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
