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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Phenol Red: 1X Liquid

Product#: DCP-DMEMH-GQR1X
$71.50
DCP-DMEMH-GQR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red Without Glucose

FluxMPS™ DCP-DMEMH-GQR1X is a Microfluidics Suitable, ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, 13C isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Glucose-free formulation (0 g/L) — supplement with D-glucose, 13C-glucose, galactose, or fructose at your defined concentration for full carbon-source control
  • L-Glutamine and Phenol Red omitted — add fresh L-glutamine (or a stabilized dipeptide) at time of use; the phenol red-free base supports fluorescence and biosensor imaging without dye interference
  • 25 mM HEPES (5958 mg/L, pKa 7.3 at 37°C) provides supplemental pH stability for brief CO₂-free sampling, alongside 3700 mg/L sodium bicarbonate for standard incubator culture
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — Microfluidics Suitable for organ-on-a-chip and tissue-chip perfusion systems
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC and fill at Diagnocine, Totowa, NJ
  • 31 total components (7 inorganic salts, 14 amino acids, 7 vitamins, 3 others) supplied with CAS numbers for full traceability
  • Custom glucose concentrations, carbon sources, and nutrient modifications available on request — contact support@diagnocine.com
CAT. NO.
DCP-DMEMH-GQR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Phenol Red: 1X Liquid
  • GlucoseNot included — researcher-defined
  • L-GlutamineNot included — add fresh or as GlutaMAX
  • Sodium Pyruvate110 mg/L
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO

Pack sizes: 500 mL, 1000 mL.

Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose, while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification limits inflammatory confounds in metabolic readouts.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a USP <788> Method 1 particulate release specification (NMT 25/mL ≥10 µm; NMT 3/mL ≥25 µm) reduce the risk of channel and valve fouling in microfluidic systems.

target

Total metabolic control

Glucose is absent by design. Add D-glucose, galactose, or 13C-glucose at a defined concentration for Warburg-effect, OXPHOS-switching, or isotope-tracing protocols — sodium pyruvate (110 mg/L) is already included as a base energy source.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon (TOC) content, supporting sensitive analytical workflows.

visibility

Low background for imaging

Phenol red-free formulation removes one major source of assay interference for confocal microscopy and optical biosensors. Note: riboflavin (0.400 mg/L), a standard DMEM component, contributes a low level of intrinsic fluorescence typical of this medium class — the particulate baseline itself is minimized by the 0.04 µm final filtration stage.

science

Rich, stable nutrient profile

Dulbecco's Modification carries roughly 4× the amino acid and vitamin concentration of the original Eagle's MEM formulation, released on a micro-batch, per-lot QC basis.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter/final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions. Reducing particulate and endotoxin load is important for glucose-free metabolic assays, where either variable can independently affect glycolytic readouts.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm final filter from early fouling.

  2. 2

    0.04 µm Final filtration I

    Retains mycoplasma-sized organisms (0.2–0.3 µm) and fine particulates not retained by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of ISO Class 5 aseptic fill & finish.

Why filtration purity matters for metabolic assays

Elevated endotoxin and subvisible particulate levels are known activators of TLR4/NF-κB signalling, which can independently affect glycolytic and OXPHOS readouts in cancer and immune cell studies. FluxMPS™ DCP-DMEMH-GQR1X is released against a < 0.05 EU/mL endotoxin specification and a USP <788> Method 1 particulate specification (NMT 25/mL ≥10 µm; NMT 3/mL ≥25 µm).

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across two filtration pairs
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration; this is a filtration control, not a per-lot USP <63> mycoplasma test.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMH-GQR1X Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Phenol Red, 1X Liquid, Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish, Microfluidics Suitable glucose-free DMEM HEPES for organ-on-a-chip applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GQR1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GQR1X is purpose-built for experiments where carbon source identity must be controlled — from Warburg-vs-OXPHOS switching studies to 13C metabolic flux analysis and glucose-dose response studies on organ-on-a-chip platforms.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactor and robotic liquid-handling platforms requiring even lower particulate load, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available.

  • Total Particulate Exclusion: 0.01 µm final cut-off for the most particulate-sensitive automated systems
  • Valve & Sensor Protection: Minimizes fouling risk in fine-gauge robotic dispensing hardware
  • Extended Perfusion Stability: Supports long-duration, unattended perfusion protocols

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Defined carbon source, low particulate, and low endotoxin support long-term culture on microfluidic organ-on-a-chip and tissue-chip platforms.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without a media change.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Glucose-free base enables galactose-forced OXPHOS maturation protocols for iPSC-derived cardiomyocytes and hepatocytes.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Defined glucose supplementation supports dose-response studies in endothelial and primary hepatocyte cultures.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Add U-13C-glucose at a defined specific activity for controlled isotope-tracing experiments via NMR or LC-MS/MS. Not compatible with Agilent Seahorse XF assays, which require a bicarbonate-free, phenol red-free base medium; this formulation contains 3700 mg/L sodium bicarbonate.

13C tracingNMR metabolomicsLC-MS/MS
Live-Cell Imaging

Microscopy & Optical Sensing

Phenol red-free base supports confocal imaging and optical biosensor platforms; low particulate baseline (USP <788>) reduces background scatter.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red, [-] Glucose
Appearance Colorless to pale yellow, clear solution
Glucose Not included — add at desired concentration
L-Glutamine Not included — add fresh or as GlutaMAX
Sodium Pyruvate 110 mg/L
Phenol Red Not included
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H₂O
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release — see §Manufacturing)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement ~10% CO₂ recommended (derived from 3700 mg/L sodium bicarbonate for pH 7.4); 25 mM HEPES provides supplemental pH stability during brief CO₂-free intervals
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

31 total components across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), presented in 3 navigable tabs, verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.00
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For 13C tracing add U-13C-glucose. For OXPHOS forcing add D-galactose (approximately 2000 mg/L). Contact support@diagnocine.com for custom carbon-source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC and fill at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water with controlled trace-metal and organic-carbon (TOC) content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL, tested per manufacturing batch prior to release.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQR1X compares

FluxMPS™ DCP-DMEMH-GQR1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GQR1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Glucose-free DMEM + 25 mM HEPES (dual HEPES/bicarbonate buffered, phenol red-free) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm stage) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Yes cancel No cancel No
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified Not specified
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes Limited Limited
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GQR1X glucose-free DMEM + 25 mM HEPES.

Yes. The glucose-free, HEPES-supplemented formulation lets researchers define carbon source identity and concentration precisely for each chip experiment, and the quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reduces particulate load below levels that risk clogging microfluidic channels and valves. Combined with a < 0.05 EU/mL endotoxin release specification, this formulation supports organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and other microphysiological system (MPS) platforms where metabolic control and channel integrity are both required.
DCP-DMEMH-GQR1X is processed through two paired prefilter/final-filter stages (a 0.1 µm prefilter followed by a 0.04 µm final filter, run twice) rather than a single 0.22 µm pass. The 0.1 µm stages provide mycoplasma-retentive filtration, and the 0.04 µm final stages reduce subvisible particulate counts beyond what a single 0.22 µm filter achieves. Every batch is released against a USP <788> Method 1 particulate specification and a < 0.05 EU/mL endotoxin specification (USP <85>).
Glucose is omitted so researchers can define carbon source identity and concentration for each protocol: add D-glucose (typically 1000 mg/L for low-glucose or 4500 mg/L for high-glucose conditions), U-13C-glucose for isotope tracing, or D-galactose (approximately 2000 mg/L) to force oxidative phosphorylation. L-glutamine and phenol red are also omitted; add L-glutamine (or a stabilized dipeptide substitute) fresh at time of use. Sodium pyruvate (110 mg/L) is already included as an alternative carbon/energy source. Contact support@diagnocine.com for custom carbon-source pre-formulations.
Yes, standard incubator culture requires approximately 10% CO₂ to maintain pH 7.4 with the 3700 mg/L sodium bicarbonate buffer in this formulation. The 25 mM HEPES component provides supplemental pH stability during brief CO₂-free intervals, such as bench-top sampling or short imaging sessions, but does not replace the incubator CO₂ requirement for extended culture. Note: this bicarbonate/HEPES dual-buffered formulation is not compatible with Agilent Seahorse XF assays, which require a bicarbonate-free, unbuffered base medium.
Yes. Serum and other protein-containing supplements should be added using a 0.2 µm low-protein-binding PES or PVDF filter to preserve high-molecular-weight serum components (immunoglobulins, lipoproteins) during sterile addition. Defined, protein-free additions (e.g., small-molecule carbon sources, salts) may be filtered at 0.1 µm. Do not use a 0.04 µm filter for supplement addition; that pore size is reserved for the base medium's final polish and will retain or foul on serum proteins and lipoproteins.
Every batch of DCP-DMEMH-GQR1X is tested by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet a release specification of < 0.05 EU/mL before release. Endotoxin is controlled per manufacturing batch rather than per unit; a Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA is available per lot, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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