Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

This FluxMPS™ DMEM formulation is Microfluidics Suitable, ultra-filtered through Diagnocine's quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Buffered with 25 mM HEPES and formulated without glucose, sodium pyruvate, sodium bicarbonate, or phenol red, it is a defined basal platform that gives investigators full control over carbon source for metabolic and nutrient-restriction research.

• Glucose-free, sodium pyruvate-free, and sodium bicarbonate-free basal formulation for studying cellular metabolism under nutrient-restricted or carbon-source-defined conditions
• 25 mM HEPES buffering (5958 mg/L) helps stabilize pH outside strict CO₂-controlled incubator environments
• L-Glutamine retained at 584 mg/L (≈4 mM) for direct use without additional glutamine supplementation
• Quadruple-stage filtration — 0.1 µm membrane twice and 0.04 µm membrane twice — exceeding a standard single-pass 0.22 µm filtration process
• Phenol red-free formulation removes a source of background absorbance/fluorescence for optical, biosensor, and imaging-based assays
• Manufactured under an ISO 13485:2016-aligned quality management system; final packaging, QC and customization performed at Diagnocine Precision, Totowa, NJ
• Batch-release endotoxin specification: less than 0.05 EU/mL (LAL, USP <85>)
• Custom pH, reintroduction of glucose/pyruvate/bicarbonate, and other supplement formulations available on request

Engineered where standard media fails

Conventional 0.22 µm filtered media leave mycoplasma-scale organisms, sub-micron particulates, and protein aggregates behind — a tolerable margin in a flask, but a direct threat to narrow microfluidic channels, sensor surfaces, and optical assays. FluxMPS™ media are processed further to remove what a standard filter cannot.

Quadruple-stage filtration system

Every FluxMPS™ Sterile-processed medium passes through a validated, four-pass filtration train — two dedicated prefilter/final-filter pairs in series — reaching a 0.04 µm final pore size well below the mycoplasma-retentive 0.1 µm grade and the industry-conventional 0.22 µm grade.

1. 1

   0.1 µm Prefiltration I

   Removes large particulate, cell debris, and protein aggregates, protecting the first 0.04 µm cartridge downstream.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass through.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of the first pass, but redundant protection for the second.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter prior to aseptic fill and finish.

Where this formulation is used

A glucose-free, pyruvate-free, bicarbonate-free, HEPES-buffered basal medium supports research where carbon source, buffering system, and optical background must be defined by the investigator rather than fixed by the formulation.

Automated Bioreactors & Robotics

For automated perfusion systems, closed-loop bioreactors, and robotic liquid handling where valve and sensor fouling drive downtime, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a distinct, six-stage cascade beyond the Microfluidics Suitable 0.04 µm line described on this page.

• Total Particulate Exclusion — six-stage cascade down to 0.01 µm for the most sensitive automated flow paths
• Valve & Sensor Protection — minimizes fouling of microvalves, flow sensors, and optical windows in closed-loop systems
• Extended Perfusion Stability — supports longer unattended run times between service intervals

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this grade for your platform.

Full parameter reference

Available pack sizes: 500 mL, 1000 mL. All parameters below reflect the 1X liquid, ready-to-filter-supplement formulation as released by Diagnocine.

Full composition (mg/L)

Formulated at four-fold BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate, released per lot. Total: 30 components across 4 categories.

Manufacturing & compliance

Every lot is manufactured, filtered, and released against a fixed set of physical, chemical, and microbiological specifications.

How DCP-DMEMH-GPBR1X compares

A side-by-side view of FluxMPS™ against conventional 0.22 µm filtered DMEM alternatives.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about DCP-DMEMH-GPBR1X.

Supporting literature

Selected literature relevant to this formulation's applications in metabolic research, microfluidics, and organ-on-a-chip systems.

1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
2. Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029-1033. doi:10.1126/science.1160809
3. Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011;21(12):745-754. doi:10.1016/j.tcb.2011.09.005
4. Good IJ. HEPES and related buffers in biological research. Biochemistry. 1966;5(2):467-477. doi:10.1021/bi00866a011
5. Zheng F, et al. Organ-on-a-chip systems: microengineering to biomimic living systems. Small. 2016;12(17):2253-2282. doi:10.1002/smll.201503208
6. Buchanan CF, et al. Flow shear stress regulates endothelial barrier function and expression of angiogenic factors in a 3D microfluidic tumor vascular model. Cell Adh Migr. 2014;8(5):517-524. doi:10.4161/19336918.2014.970001
7. Buescher JM, et al. A roadmap for interpreting 13C metabolite labeling patterns from cells. Curr Opin Biotechnol. 2015;34:189-201. doi:10.1016/j.copbio.2015.02.003
8. Wenger RH, et al. Frequently asked questions in hypoxia research. Hypoxia (Auckl). 2015;3:35-43. doi:10.2147/HP.S92198
9. Srinivasan B, et al. TEER measurement techniques for in vitro barrier model systems. J Lab Autom. 2015;20(2):107-126. doi:10.1177/2211068214561025
10. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 7th ed. Wiley-Blackwell; 2016. doi:10.1002/9781118873651