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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Formulated without glucose or sodium bicarbonate and buffered with 25 mM HEPES for CO₂-independent culture.
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, including 0.1 µm mycoplasma-retentive stages
- Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test)
- Formulated without glucose and without sodium bicarbonate; buffered with 25 mM HEPES for CO₂-independent culture
- Contains L-Glutamine (584 mg/L) and Sodium Pyruvate (110 mg/L) as defined nitrogen/carbon sources
- Prepared using Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control
- Manufactured under an ISO 13485:2016 quality management system; ISO Class 5 (Class 100) aseptic fill & finish
- Custom pH, glucose, HEPES, salts & nutrients available on request — support@diagnocine.com
- GlucoseRemoved
- L-Glutamine584 mg/L ([+])
- Sodium Pyruvate110 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)310 - 350 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, away from bright light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
A 0.04 µm final filtration stage removes sub-micron particulates and microaggregates that pass through conventional 0.22 µm filters, supporting unobstructed flow in narrow microfluidic channels.
Total metabolic control
Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for consistent trace-metal and organic-carbon control, lot to lot.
Low background for imaging
Ultra-low particulate baseline from the 0.04 µm final filtration supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
This formulation is manufactured with a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — well beyond the reach of conventional 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries.
-
2
0.04 µm Final filtration I
Retains fine particulates and sub-micron material, including 0.1 µm mycoplasma-retentive filtration — entirely absent from standard 0.22 µm media.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge for redundant filtration capacity.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
© Diagnocine® — DCP-DMEMH-GB1X
Optimized for next-generation cell biology platforms
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
For automated perfusion bioreactors and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available, adding 0.02 µm and 0.01 µm stages after the standard 0.04 µm polish.
- Total Particulate Exclusion — minimizes valve, sensor, and micro-pump fouling in long-run automated systems
- Valve & Sensor Protection — reduces risk of optical and electrochemical sensor drift from particulate buildup
- Extended Perfusion Stability — supports multi-week unattended perfusion runs
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.
Micro Physiological System (MPS) & Chip
Ultra-low particulate media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Glucose-free formulation for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined, glucose-free formulation supports ¹³C metabolic tracing and NMR metabolomics workflows where exogenous carbon-source control is required. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate: 1X Liquid is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Phenol Red, HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Sodium Bicarbonate, Glucose |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310 - 350 mOsm/kg H₂O |
| Glucose | Removed |
| L-Glutamine | 584 mg/L ([+]) |
| Sodium Pyruvate | 110 mg/L ([+]) |
| Phenol Red | 15.9 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, away from bright light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | HEPES-buffered; reduced CO₂ dependence (validate per cell line) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Traceability | Full lot traceability; CoA available on request |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
| Pack sizes | 500 mL, 1000 mL |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 32 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-45-6 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with low trace-metal and organic-carbon content, supporting consistent water quality lot to lot.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains through final fill.
Micro-Batch Precision
Small-batch production with tight process control: osmolality (±5 mOsm/kg), pH (±0.05) — supporting reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay. Assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count testing performed per lot; results reported on the Certificate of Analysis.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 310 - 350 mOsm/kg H₂O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-GB1X compares
Critical advantages in filtration, mycoplasma-retentive filtration, water quality, and QC depth that conventional media cannot match for MPS and microfluidic applications.
| Parameter | DCP-DMEMH-GB1X (FluxMPS™) | Standard DMEM (0.22 µm) | Competitor DMEM (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation definition | [+] L-Glutamine, Phenol Red, HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Sodium Bicarbonate, Glucose | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Method 1 tested per lot | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate: 1X Liquid (DCP-DMEMH-GB1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
