Defined medium for late stage porcine embryos (PBM)

(PBM <Late stage porcine embryo culture medium>)

Defined medium for late stage porcine embryos (PBM)
FNK-IFP1030P	Size: 25mLx2	Current Page
Defined medium for late stage porcine embryos (PBM) PBM is a medium that has added glucose and glycine in the defined medium for porcine embryos (PZM-5). PBM medium takes advantage for an improved embryo development after the morula stage, the increased blastomere numbers of blastocyst and the increased rate of hatched blastocyst formation. This medium effectively supports the embryo development under a low oxygen condition (5% O2/5% CO2/90% N2, 39°C).
Basic Culture Kit for Porcine Embryos in vitro
FNK-IFP100PEP-BK	Size: 1 kit
This is a basic culture kit for porcine embryos in vitro containing porcine oocyte/embryo collection medium (POE-CM), basic medium for porcine oocyte maturation (POM), porcine fertilization medium (PFM), defined medium for porcine embryos (PZM-5), dbcAMP concentrated solution-100X (dbcAMP-100X) and Reproplate. This kit can efficiently produce transferable embryos (blastocysts) under a low oxygen condition (5% O2/5% CO2/90% N2, 39°C), without the coculture of cumulus/granulose cells. POE-CM (100 mL x 3) POM (25 mL x 2) PFM (100 mL x 1) PZM-5 (25 mL x 1) dbcAMP (100 times solution) (0.3 mL x 1) Repro Plate (10 sheets ) × 3 pack)
Complete Culture Kit for Porcine Embryos in vitro
FNK-IFP101PEP-CK	Size: 1 kit
This is a complete culture kit for porcine embryos in vitro containing porcine oocyte/embryo collection medium (POE-CM), basic medium for porcine oocyte maturation (POM), porcine fertilization medium (PFM), defined medium for porcineembryos (PZM-5), defined medium for late stage porcine embryos (PBM), dbcAMP concentrated solution-100X (dbcAMP-100X) and Reproplate. This kit can efficiently produce transferable embryos (blastocysts) under a low oxygen condition (5% O2/5% CO2/90% N2, 39°C) without the coculture of cumulus/granulosa cells. This kit can support to increase the blastomere numbers of blastocysts and the formation of hatched blastocysts. POE-CM (100mL x 3) POM (25mL x 2) PFM (100mL x 1) PZM-5 (25mL x 1) PBM (25mL x 1) dbcAMP (100 times solution) (0.3mL x 1) Book) Repro plate (10 sheets x 3 packs)
Porcine oocyte/embryo collection medium (POE-CM)
FNK-IFP1040P	Size: 100mLx5
This medium kept warm to about 37°C is aseptically dispensed to sterile bottle or syringe and is used for collection or washing of porcine oocytes and embryos by the method of dissecting or aspirating ovaries. POE-CM is a suitable medium for collection or washing of porcine oocytes and embryos and is a complete synthetic medium that added phenol red to PXE-Hepes (Yoshioka.K., Et al J. Reprod. Dev., 54: 208-213, 2008). This medium contains mineral salts, sugars (lactic acid and pyruvic acid), polyvinyl alcohol, Hepes and sodium bicarbonate, etc.
Basic medium for porcine oocyte maturation (POM)
FNK-IFP1010P	Size: 25mLx3
To mature porcine oocytes. This is a modification medium which added polyvinyl alcohol to the original medium for porcine oocyte maturation (POM) (Yoshioka.K., Et al J. Reprod. Dev., 54: 208-213, 2008) and is a complete synthetic basic medium for oocyte maturation. This medium effectively supports porcine oocyte maturation under a low oxygen condition (5% O2/5% CO2/90% N2, 39°C). Usage: Please add 10 IU /mL equine chorionic gonadotropin (eCG) + 10 IU /mL human chorionic gonadotropin (hCG) to the POM medium during porcineoocyte maturation culture. There is no need to add porcine follicular fluid.
High Performance Basic Medium for Porcine Oocyte Maturation (HP-POM)
FNK-IFP1011P	Size: 25mLx3
Serum-free basal medium optimized for egg maturation, a high-performance medium supplemented with TGF-α and other basic medium for porcine ovum maturation Using this medium, the oocyte maturation rate is almost the same as that of the POM medium, but it has been reported that the embryo development rate (blastocyst development rate) after in vitro fertilization is high (Mito et al.). This medium is sterilized and can be used as is. The use of this medium effectively promotes egg maturation by performing low oxygen culture (5% O2/5% CO2/90% N2, 39°C). Note: Please add 10 IU /mL equine chorionic gonadotropin (eCG) + 10 IU / ml human chorionic gonadotropin (hCG) to HP-POM medium during porcine oocyte maturation culture. There is no need to add porcine follicular fluid. HP-POM medium is for research use only. Do not use in animals, humans, or diagnostic procedures.
dbcAMP concentrated solution-100X
FNK-IFP1060P	Size: 0.5mLx1
It is effective for normal maturation of porcine ovum by adding to basal medium for porcine ovum maturation (POM) or basic medium for maturation of porcine ovum (HP-POM) used for ovum maturation culture. This concentrate is sterilized and can be used by adding it directly to the maturation medium.
SEM-5X (semen dilution for medium)
FNK-IFP1050P	Size: 25mLx1
For washing and dilution of sperm used in insemination and preparation of isotonic percoll solution. Sterilized and ready to use.This can be used for the washing and dilution of sperm used in insemination and the preparation of isotonic percoll solution. This is sterilized ready to use.
Porcine fertilization medium (PFM)
FNK-IFP1020P	Size: 100mLx2
A serum-free basic medium for porcine oocyte maturation PFM is a complete synthetic medium that based on PGMtac4 (Yoshioka.K., Et al J. Reprod. Dev., 54: 208-213, 2008) and is optimized to porcine in vitro fertilization. This medium is sterilized and effectively supports in vitro fertilization under a low oxygen condition.
Defined medium for porcine embryos (PZM-5)
FNK-IFP0410P	Size: 25mLx3
Medium to culture porcine embryos PZM-5 medium is an improved defined medium developed from the previous PZM-4 medium (Yoshioka.K., Et al J. Reprod. Dev., 54: 208-213, 2008) for porcine embryo culture. This is useful for the culture of in vitro-derived embryos and in vivo-derived embryoscollected after an artificial insemination. This medium is sterilized and effectively supports porcine embryo development under a low oxygen condition (5% O2/5% CO2/90% N2, 39°C). Note: PZM-5 medium is for research use only. Do notuse in animals, humans, or diagnostic procedures.
Pig embryo vitrification preservation solution kit (PEV-SK)
FNK-IFP16PVSK	Size: 1 kit
A vitrification preservation solution kit used for ultra-low temperature preservation of pig embryos. It is a set of primary equilibrium solution, secondary equilibrium solution, vitrification solution, and heating / diluting solution. The product specifications are all composed of known ingredients.

1. In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Medium (PBM).Mito T, Hoshi H., Methods Mol Biol. 2019;2006:105-113. PMID: 31230275
2. Birth of piglets from in vitro-produced porcine blastocysts vitrified and warmed in a chemically defined medium. Mito T, Yoshioka K, Noguchi M, Yamashita S, Misumi K, Hoshi T, Hoshi H. Theriogenology. 2015 Nov;84(8):1314-20. PMID: 26255223
3. Recombinant human follicle-stimulating hormones and transforming growth factor-alpha enhance in vitro maturation of porcine oocytes. Mito T, Yoshioka K, Noguchi M, Yamashita S, Hoshi H.  Mol Reprod Dev 2013;80:549-560.  PMID: 23661505
4. Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium. Mito T, Yoshioka K, Yamashita S, Suzuki C, Noguchi M, Hoshi H.   Reprod Fertil Dev. 2012;24(3):443-50. PMID: 22401276
5. Transforming growth factor-α in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure. Mito T, Yoshioka K, Nagano M, Suzuki C, Yamashita S, Hoshi H.  Theriogenology 2009; 72: 841-850. PMID: 19632712
6. Defined system for in vitro production of porcine embryos using a single basic medium.Yoshioka K, Suzuki C, Onishi A.  J Reprod Dev 2008; 54: 208-213. PMID: 18408352
7. Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos. Suzuki C, Yoshioka K, Sakatani M, Takahashi M.  Zygote 2007; 15: 317-324. PMID: 17967211
8. Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions. Karja NW1, Kikuchi K, Fahrudin M, Ozawa M, Somfai T, Ohnuma K, Noguchi J, Kaneko H, Nagai T. Reprod Biol Endocrinol. 2006 Nov 6;4:54 PMID: 17087833
9. Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium. Suzuki C, Yoshioka K.  Reprod Fertil Dev 2006; 18: 789-795. PMID: 17032588
10. Birth of piglets through the non-surgical transfer of blastocysts produced in vitro. J Reprod Dev. Suzuki C1, Iwamura S, Yoshioka K.  2004 Aug;50(4):487-91. PMID: 15329482 
11. Production of piglets derived from in vitro-produced blastocysts fertilized and cultured in chemically defined media: effects of theophylline, adenosine, and cysteine during in vitro fertilization. Yoshioka K, Suzuki C, Itoh S, Kikuchi K, Iwamura S, Rodriguez-Martinez H.  Biol Reprod 2003; 69: 2092-2099. PMID: 12930720 
12. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Yoshioka K, Suzuki C, Tanaka A, Anas IMK, Iwamura S.  Biol Reprod 2002; 60: 112-119. PMID: 11751272
13. Long-term preservation test of swine liquid semen using various diluted stock solutions. Masaru Sone, Mikio Chihisa, Mitsutoshi Yoshida, Kimio Banba, Jun Ogasa.  The Japan Pig Association 1992; 29: 41-50. (in Japanese only)