Columbia Agar Base Plates/W Carbenicillin
Catalog No: DCP-CABPG
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 50 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Columbia Agar Base with carbenicillin is a nutrient-rich, non-blood agar medium in which standard Columbia agar base is supplemented with the β-lactam antibiotic carbenicillin to introduce selectivity through inhibiting carbenicillin-susceptible bacteria while permitting growth of carbenicillin-resistant organisms. Columbia agar base is a complex peptone medium originally devised as a blood-agar base for the cultivation of fastidious organisms such as streptococci, staphylococci, Neisseria, and Haemophilus, as well as non-fastidious bacteria. The peptones and yeast extract provide amino acids, peptides, nitrogen, B-complex vitamins, and other growth factors that support bacterial growth and well-defined colony morphology. Starch works as a carbon and energy source and also is able to help neutralize toxic metabolites, aiding the recovery of more delicate organisms, while NaCl maintians the osmotic balance.
Carbenicillin is a broad-spectrum β-lactam (a carboxypenicillin) that inhibits bacterial cell wall synthesis by binding penicillin-binding proteins and blocking peptidoglycan cross-linking, leading to lysis of actively growing susceptible cells. When carbenicillin is added to Columbia Agar Base, the medium becomes selective by inhibiting many carbenicillin-susceptible Gram-negative rods and Gram-positive bacteria, while organisms that are intrinsically resistant or carry β-lactamase or other carbenicillin-resistance determinants can still grow.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
