Blasticidin: 1000X solution
Cat No. DCP-10BSH1000X_10 mL
Storage: -20°C (protect from light)
Size: 10 mL
Concentration: 10mg/mL
Sterile: Filtered using 0.1-micron membrane
PubChem ID: Blasticidin S Hydrochloride, 356629
UN Number: 2811
Formula: C17H26N8O5 HCl
MW: 458.9 g/mol
Description:
Balsticidin S HCL is a nucleoside antibiotic which is isolated from the soil bacterium Streptomyces griseochromogenes. Nucleoside analogue antibiotics resemble cytidine which has been effective to disrupt protein translation in human cells, fungi, and bacteria. It acts as an inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. It inhibits protein synthesis by blocking peptide bond formation and hydrolysis of peptidyl-tRNA. Particularly, it binds to the ribosomal P-site which strengthens the binding of tRNA and slows down or prevents the subsequent addition of amino acids to the growing polypeptide chain. This process blocks the hydrolysis of peptidyl-tRNA due to the large ribosomal subunit's increased affinity for tRNA. Cells can be made resistant by expressing the bsr or BSD deaminase genes. The resistance is shown to convert it into a nontoxic deaminohydroxy derivative. It has shown potent in vitro activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans among others. However, it has relatively low activity against Enterobacteriaceae like E. coli.
To determine if antibiotics are effective against certain bacterial species, clinical in vitro assays called antimicrobial susceptibility tests, or ASTs, are frequently performed. Medical microbiologists test them utilizing panels, discs, and MIC strips against gram-negative and gram-positive bacteria. The results of ASTs are used in clinical settings to determine which antibiotic(s) to prescribe for various infections as these tests decrease the risk of using an antibiotic against bacteria exhibiting resistance to it.
Application:
It is used as a selection agent for cells expressing resistance genes, like bsr, bis, and BSD genes.
It can be used to prevent cell culture contamination as it inhibits the growth of susceptible cells.
It is useful for studying protein synthesis mechanisms as it inhibits the protein synthesis.
It is also useful in gene editing applications like CRISPR-Cas9 as it can be used as a selectable marker to identify and isolate cells that have successfully undergone gene editing.
* Blasticidin solution should not be subjected to multiple freeze/thaw cycles and should not be stored in a frost-free freezer.
* Blasticidin is a harmful compound, therefore please take precautions when handling it like using a fume hood and wearing personal protective equipment. Do not ingest or inhale the powder. If ingested, immediately call a poison control center or doctor.
Storage and Shelf Life:
- Storage: -20 degree Celsius (away from light)
- Shelf life: 9 months
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
