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Blasticidin: 1000X solution

Product#: DCP-10BSH1000X_10mL
$411.37
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
Product Overview
ISO 13485 Certified Manufacturing

FluxMPS™ Blasticidin: 1000X solution

FluxMPS™ Blasticidin: 1000X solution is a single-stage, 0.1 µm membrane-filtered antibiotic selection reagent supplied at 10 mg/mL for reliable selection of cells expressing blasticidin-resistance genes. Isolated from Streptomyces griseochromogenes, it inhibits protein synthesis in both prokaryotic and eukaryotic cells, making it a dependable tool for stable cell line generation, CRISPR-Cas9 marker selection, and standard, microfluidic, or organ-on-a-chip (OoC) culture workflows.

  • 10 mg/mL Blasticidin S HCl supplied as a 1000X concentrated stock solution for direct dilution into culture medium
  • Filtered using a single 0.1 µm membrane pass for particulate and mycoplasma-scale contamination control
  • Nucleoside antibiotic isolated from Streptomyces griseochromogenes; blocks peptide bond formation and peptidyl-tRNA hydrolysis at the ribosomal P-site
  • Effective selection agent for cells expressing bsr, bsd, or BSD resistance genes, including CRISPR-Cas9 edited cell isolation
  • Manufactured under an ISO 13485:2016 quality management system; final packaging, QA, and customization completed at Diagnocine, Totowa, NJ
  • Supplied in 10 mL format; store at -20°C protected from light; avoid repeated freeze/thaw cycles and frost-free freezers
  • Custom concentrations, formats, and packaging available on request
SKU: DCP-10BSH1000X_10mL Cell Culture Media · Selection Antibiotics UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Blasticidin: 1000X Solution (10 mg/mL), 10 mL
  • Concentration10 mg/mL (1000X)
  • Molecular FormulaC17H26N8O5 · HCl
  • Molecular Weight458.9 g/mol
  • Filtration0.1 µm membrane, single-stage
  • PubChem CID356629
  • UN Number2811
  • Storage-20°C, protect from light
  • Shelf Life9 months from date of manufacture, unopened
  • ShippingContact for specification
  • Pack Size10 mL
ISO 13485:2016 0.1 µm Filtration RUO
Why FluxMPS™

A selection antibiotic built for traceable, reproducible research

Reagent-grade selection antibiotics must combine consistent potency with manufacturing traceability. Conventional blasticidin preparations vary lot to lot and rarely disclose filtration or QC detail, increasing the risk of inconsistent selection pressure or contamination in sensitive microfluidic and organ-on-a-chip cultures.

filter_alt

Membrane-Filtered Purity

Filtered using a 0.1 µm membrane to reduce particulate and mycoplasma-scale contaminants in the 1000X stock prior to fill.

target

Consistent Selection Pressure

Manufactured to a fixed 10 mg/mL (1000X) concentration for reproducible antibiotic selection across experiments and lots.

science

Well-Characterized Mechanism[1]

A nucleoside antibiotic that binds the ribosomal P-site, strengthening tRNA binding and blocking peptide bond formation and peptidyl-tRNA hydrolysis in prokaryotic and eukaryotic cells.

biotech

CRISPR & Stable Line Selection[6]

Functions as a selectable marker for isolating cells expressing bsr, bsd, or BSD resistance genes after gene editing or stable transfection.

verified

Broad-Spectrum Antimicrobial Activity[4]

Potent in vitro activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, with comparatively low activity against Enterobacteriaceae such as E. coli.

tune

Customization on Demand

Custom concentrations, formats, and packaging available on request — contact support@diagnocine.com.

Purity Architecture

Filtration

Blasticidin: 1000X solution is manufactured with a single-pass 0.1 µm membrane filtration step, reducing particulate and mycoplasma-scale (0.2–0.3 µm) risk in the concentrated stock prior to fill.

  1. 1

    0.1 µm Filtration

    Single-pass 0.1 µm membrane filtration of the concentrated 10 mg/mL stock prior to aseptic fill into 10 mL units.

Filtration summary

Blasticidin: 1000X solution is filtered once through a 0.1 µm membrane. This is a single-stage filtration process, not a multi-pass or paired filtration train.

1Filtration pass
0.1 µmMembrane pore size
This product is filtered using a single 0.1 µm membrane pass. No per-lot USP <71> sterility test result or mycoplasma test result is asserted; filtration is a risk-reduction step, not a test outcome.
Applications

Selection, gene editing, and translation research

Blasticidin: 1000X solution is used across standard, microfluidic, and organ-on-a-chip (OoC) research to select and maintain cells carrying blasticidin-resistance genes, to isolate successfully gene-edited clones, and to study ribosomal mechanisms of protein synthesis inhibition.

Microfluidics

Micro Physiological System (MPS) & Chip

Used as a stable selection agent in engineered cell lines cultured within microfluidic and organ-on-a-chip (OoC) systems.

OoCToCBoCLoCMPS
Cell Line Selection

Stable & Transient Selection

Applies selective pressure to identify and maintain cells expressing blasticidin-resistance genes.

bsrbsdBSD
Gene Editing

CRISPR-Cas9 & Genome Engineering[6]

Serves as a selectable marker to isolate cells that have successfully undergone gene editing.

CRISPR-Cas9Knock-inKnock-out
Protein Synthesis Research

Translation & Ribosome Studies[1]

Used to study inhibition of peptide bond formation and ribosomal mechanisms in prokaryotic and eukaryotic systems.

Peptidyl-transferaseP-site bindingtRNA hydrolysis
Microbiology

Antimicrobial Susceptibility Testing[4]

Applied in antimicrobial susceptibility testing (AST) against gram-positive and gram-negative organisms.

MICDiscsPanels
Contamination Control

Cell Culture Maintenance

Helps prevent overgrowth of non-resistant or contaminating cells by inhibiting the growth of susceptible cells in culture.

Selective pressureCulture purity
Technical Specifications

Specifications

Key physical, chemical, safety, storage, and traceability parameters for Blasticidin: 1000X solution.

Physical & Chemical Parameters
Parameter Specification
Active Compound Blasticidin S Hydrochloride, 10 mg/mL (1000X)
Molecular Formula C17H26N8O5 · HCl
Molecular Weight 458.9 g/mol
Concentration 10 mg/mL (1000X)
Purity, Identification & Safety Parameters
Parameter Specification
Filtration Single-stage 0.1 µm membrane (single pass)
PubChem CID 356629
UN Number 2811
Hazard / Handling Harmful compound; use fume hood and PPE; do not ingest or inhale
Storage, Handling & Logistics
Parameter Specification
Storage Temperature -20°C, protect from light
Freeze/Thaw Handling Avoid multiple freeze/thaw cycles; do not store in a frost-free freezer
Shelf Life 9 months from date of manufacture, unopened
Shipping Condition Contact for specification
Raw Materials & Regulatory Traceability
Parameter Specification
Raw Material Grade Research Use Only (RUO)
Traceability Lot-specific Certificate of Analysis available
Manufacturing QMS ISO ISO 13485:2016 aligned
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment ISO 13485:2016 aligned
Production Method Custom assembly & packaging at Diagnocine, Totowa, NJ
Intended Use Research Use Only (RUO)
Formulation

Full composition

Blasticidin: 1000X solution is a single active-compound antibiotic solution. Composition, chemical identifiers, and stability handling details are provided below.

Component CAS Number Concentration
Blasticidin S Hydrochloride 3513-03-9 10 mg/mL (1000X)
Property Value
Molecular Formula C17H26N8O5 · HCl
Molecular Weight 458.9 g/mol
PubChem CID 356629
UN Number 2811
Parameter Detail
Freeze/Thaw Avoid multiple freeze/thaw cycles
Storage Equipment Do not store in a frost-free freezer
Hazard Precaution Harmful; use fume hood and PPE; do not ingest or inhale the powder
Storage Temperature -20°C, protect from light
Custom concentrations, formats, and packaging available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & Compliance

Blasticidin: 1000X solution is manufactured, filtered, packaged, and quality-tested under a controlled quality management system.

verified_user

ISO 13485:2016 Quality System

Manufactured under ISO 13485-certified facilities (Suppliers of Diagnocine Precision).

filter_alt

Single-Stage 0.1 µm Filtration

Filtered using a 0.1-micron membrane to reduce particulate and mycoplasma-scale contamination prior to fill.

precision_manufacturing

Custom Assembly & Packaging

All specific customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.

fact_check

Final QA & Testing

All final packaging, quality assurance, and testing are completed at the Diagnocine R&D and Quality Testing Center.

science

Filtration Validation

Single 0.1 µm membrane filtration pass, documented per production batch.

shield

Handling & Safety

Classified as a harmful compound (UN 2811); requires fume hood and PPE during handling.

schedule

Storage Stability

9-month shelf life from date of manufacture when stored at -20°C, protected from light, without repeated freeze/thaw cycles.

description

Documentation / CoA

Certificate of Analysis available on request.

A Certificate of Analysis (CoA) is available on request via support@diagnocine.com.
Product Comparison

How DCP-10BSH1000X_10mL compares

Where a published, attributed comparison figure is available, it is shown; where none exists, the field is marked "Not specified" rather than estimated.

Parameter DCP-10BSH1000X_10mL (FluxMPS™) Other Suppliers
Concentration & Format 10 mg/mL (1000X), 10 mL Not specified
Filtration 0.1 µm membrane (single-pass) Not specified
Endotoxin (release specification) Not specified Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
Manufacturing QMS ISO 13485:2016 Not specified
Custom Formulation check_circle Available on request Not specified
Certificate of Analysis check_circle Available on request Not specified

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about Blasticidin: 1000X solution.

Yes. The 1000X stock is single-stage 0.1 µm membrane filtered and diluted directly into standard, microfluidic, or organ-on-a-chip (OoC) culture medium to provide selection pressure for cells expressing blasticidin-resistance genes.
The 1000X stock is filtered once through a 0.1 µm membrane, reducing particulate and mycoplasma-scale (0.2–0.3 µm) contamination risk in the concentrated solution prior to fill. This is a single filtration pass, not a multi-stage process.
Blasticidin S inhibits protein synthesis by binding the ribosomal P-site, blocking peptide bond formation and peptidyl-tRNA hydrolysis. Cells can be made resistant by expressing the bsr or BSD deaminase genes, which convert Blasticidin S into a nontoxic deaminohydroxy derivative, allowing resistant cells to survive selection while susceptible cells do not.
The Source Description does not specify a universal working concentration, as susceptibility varies by cell line and resistance construct. The 1000X stock (10 mg/mL) is provided for dilution into culture medium; we recommend an empirical kill-curve titration on untransfected cells of your specific line to determine the minimum effective concentration.
Yes, Blasticidin is typically diluted into complete, serum-containing culture medium at time of use. If additional filter-sterilization of a serum-containing mixture is required, use a 0.2 µm low-protein-binding PES or PVDF filter to avoid stripping serum proteins and growth factors. Never use a 0.04 µm filter for serum-containing supplements.
Store at -20°C, protected from light. Shelf life is 9 months from date of manufacture when unopened. Avoid multiple freeze/thaw cycles and do not store in a frost-free freezer, as repeated freeze/thaw and frost-free cycling can degrade the antibiotic.
Yes. A Certificate of Analysis is available on request via support@diagnocine.com, documenting lot-specific manufacturing and quality assurance testing completed at the Diagnocine R&D and Quality Testing Center.
Scientific References

Supporting literature

Curated literature on Blasticidin S mechanism, resistance gene selection, gene editing applications, and related methodology.

  1. Yamaguchi H, Yamamoto C, Tanaka N. Inhibition of protein synthesis by blasticidin S: studies with cell-free and reconstituted systems. J Biochem. 1965. doi:10.1093/oxfordjournals.jbchem.a128378
  2. Izumi M, Miyazawa H, Kamakura T, et al. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp Cell Res. 1991. doi:10.1016/0014-4827(91)90467-9
  3. Kimura M, Kamakura T, Tao QZ, Kaneko I, Yamaguchi I. Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker. Mol Gen Genet. 1994. doi:10.1007/BF00279458
  4. Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis. 2009. doi:10.1086/600885
  5. Low LA, Tagle DA. Organs-on-chips: progress, challenges, and future directions. Exp Biol Med. 2017. doi:10.1177/1535370217700523
  6. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013. doi:10.1038/nprot.2013.143
  7. Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Methods Mol Biol. 2011. doi:10.1007/978-1-61779-108-6_1
  8. Karreman C. Fusion PCR generates BSD-selectable expression constructs for mammalian cell lines. Gene. 1998. doi:10.1016/S0378-1119(98)00363-0

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