Anti-KDM5A/ RBP2/ JARID1A antibody, mouse monoclonal (18E8)
Product#: FNK-71-177
$346.79
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Anti-KDM5A/ RBP2/ JARID1A antibody, mouse monoclonal (18E8), KO Validated
General Information
| Cat. No. | ：FNK-71-177 |
| Size | ：50 µg |
| Reactivity | ：Human and mouse RBP2. Can detect endogenous levels of RBP2. |
| Immunogen | ：A synthetic peptide corresponding to human RBP2, amino acids 1416-1434. |
| Validation | ：Specificity was validated with KO cells (human) for Western Blotting |
| Isotype | ：Mouse IgG2a kappa |
| Form | ：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Application | ：
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| Cross Reactivity | ：Human/Mouse |
| Storage | ：Shipped at 4℃ or -20℃ and store at -20℃. |
| Data Link | ：UniProtKB/Swiss-Prot P29375 (KDM5A_HUMAN) |
Backgroud
RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase
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Fig.1 Weastern blot of RBP2 in crude cell extracts Samples: 1. HeLa control siRNA 2. HeLa RBP2 siRNA 3. MCF7 4. U2OS 5. NIH3T3 6. J1 (mouse ES) |
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| Fig.2 Immunofluorescence staining of HeLa cell with anti-RBP” antibody 1. HeLa cells were fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min.
2. Incubate cells with 1.5% BSA in PBS for 30 min to block non-specific binding of the antibodies. Incubate the cells with 1/2,000 diluted anti-RBP2 antibody (18E8) in 1% BSA in PBS at 4℃ overnight. 3. Incubate cells with a secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI |
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References: This antibody has been used in the following publication.
1. Nishibuchi G et al. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem. 2014 Oct 17;289(42):28956-70. PMID: 25190814
Related product: #71-175 anti-RBP2/ JARID1A antibody, mouse monoclonal (9A6)
Aliases for KDM5A Gene
- Lysine Demethylase 5A 2 3 5
- [Histone H3]-Trimethyl-L-Lysine(4) Demethylase 5A 3 4
- Jumonji/ARID Domain-Containing Protein 1A 3 4
- Lysine (K)-Specific Demethylase 5A 2 3
- Retinoblastoma-Binding Protein 2 2 4
- Lysine-Specific Demethylase 5A 3 4
- Histone Demethylase JARID1A 3 4
- RBBP-2 3 4
- RBBP2 3 4
- RBP2 3 4
- Jumonji, AT Rich Interactive Domain 1A (RBBP2-Like) 2
- Jumonji, AT Rich Interactive Domain 1A (RBP2-Like) 3
- Jumonji, AT Rich Interactive Domain 1A 2
- Retinoblastoma Binding Protein 2 3
- EC 1.14.11.67 4
- EC 1.14.11 51
- JARID1A 4
- KDM5A 5
