Recombinant HBsAg with high antigenic activity (HBsAg-XT）
Recombinant HBsAg with high antigenic activity (HBsAg-XT）
General information
| Cat. No. | ：FNK-BCL-AGX-02 |
| Size | ：100µg |
| Source | ：Yeast（Saccharomyces cerevisiae） |
| Appearance | ：Lyophilized white powder |
| Activity | ：1ng/mL of the product as protein concentration corresponds to 0.5 to 1.0 IU/mL of WHO HBsAg standards |
| Structure | ：Nano size particles having antigen protein floating in lipid bilayer. The mean particle size is 50 to 60nm as determined dynamic light scattering methods (20 nm as determined by electron microscopy). |
| Dissolve | ：For 100 µg vial, added 500 µL of water to the vial that makes a antigen solution at 200 μg/mL in PBS (137 mM NaCl, 8.1 mM Na2HPO4 • 12H2O, 2.68 mM KCl, 1.47 mM KH2PO4, pH 7.2 - 7.4) containing 1% sucrose. |
| Purity | ：Over 95% |
| Storage | ：-20°C (3 years guarantee after dispatch) |
| Usage | ：Standard antigen for ELISA, western, and others |
Introduction
HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials. In this page we introduce HBsAg-related antibodies. All antibodies listed here are good for western blot and ELISA.
Description
Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. Most of commercially available HBsAg is composed of either S-protein alone or a mixture of Sand M-proteins. HBV-infected patients generally possess antibody against S-protein, since most circulating antigen is S-protein. HBsAg L-protein-ST type contains all the three components in one protein. The S-protein region, however, is so modified that the regular human antibody to S-protein does not recognize. The Pre-S1 and Pre-S2 region is intact and shows high antigen activity. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection. And Pre-S2 region is also known to play important role in HBV infection. Thus, the product can be used as a unique tool to investigate the mechanism of HBV infection as well as antigens for both Pre-S1 and Pre-S2. The product is also used as an antibody-escapable mimic antigen.
Hepatitis B Virus Surface Antigen L-protein-ST type
Particulate HBsAg composed of L protein that has all three (S、Pre-S1、Pre-S2) domains, also called L antigen or large S antigen. The product is produced in recombinant yeast. Amino acids of S domain is replaced by naturally occurring escape mutants so that this antigen does not work as normal S-antigen; it is not recognized by ordinary anti-HBsAg (S) antibody and does not work as antigen to produce normal anti-HBsAg (S) antibody. Because other domains are normal and recognized as each specific antibodies, it can be used as a mimetic of HBV escape mutant or an antigen specific for Pre-S1 and Pre-S2 domains. The antigen may be useful to investigate HBV infection mechanism since it can escape from normal anti-S antibody. The genotype is C.
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Fig. 1: SDS-PAGE
HBsAg-XT was subjected for SDS-PAGE (Silver staining) at 100ng.
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Note: BNC-XT adsorb plastic surface. It is recommended to use low-protein binding tubes for dilution.
About Product #: BCL-AGX-01 is no longer available. The product coded BCL-AGX-02 changed the content to 100 μg and lowered price. BCL-AGX-03 is produced upon request. For the detail visit our website.
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