pEU Vector Set

Cat. No.: FNK-CFS-PEU-v1.0

This Product is for research use only.


Volume and Concentration : 10 µL each  [0.3 mg/ml]

Size: 1 Set


Description: 

With the pEU Vector Set we are providing dedicated expression vectors for use in the wheat germ cellfree protein expression system. This includes vectors for working with the GST-, His, or DYKDDDDK-affinity tag as well as the positive control vector pEU-E01-DHFR for testing your reaction conditions. Each vector has an SP6 RNA polymerase promoter to drive RNA expression followed by the E01 enhancer. The E01 enhancer is required to initiate the translation reaction. The coding region for the protein of interest can be cloned into any of the restriction sites within the multi cloning site of the vectors. However, we advise to use the closest possible restriction site near the E01 enhancer to assure effective initiation of translation. The SP6 RNA polymerase does not require any terminator sequence to stop transcription.

Inserts can be cloned into the vectors using standard methods for DNA recombination. Please assure during vector design that your insert encodes for a proper starting methionine and has an in frame stop codon at the end; pEU vectors do not provide stop codons after the multi cloning site. Assure further that inserts are in frame when using a vector encoding for an affinity tag at the N terminus. A starting methionine may not be required when cloning in frame with an affinity tag at the N terminus. Similarly, do not include any stop codon at the end of your insert when cloning in frame with an affinity tag at the C terminus.

All our vectors have an ampicillin resistance marker. After transformation bacteria can be grown on Lysogeny Broth (LB) medium with added ampicillin at a concentration of 100 μg/mL. The vectors are highcopy vectors and should commonly give good DNA yields using regular plasmid DNA purification kits. Confirm DNA purity before use in a protein expression experiment. It may be necessary to further purify the plasmid DNA by phenol/chloroform extraction if no protein expression is observed. Refer to our other manuals for more information on the DNA requirements for protein synthesis.


Vectors provided
The table below lists the vectors included in the vector set.
 

No.	Vector Name			Volume	Quantity	Storage
1	pEU-E01-MCS		0.3 mg/mL	10 μL	1	-20℃
2	pEU-E01-His-TEV-MCS-N1	N set	0.3 mg/mL	10 μL	1	-20℃
3	pEU-E01-His-TEV-MCS-N2		0.3 mg/mL	10 μL	1	-20℃
4	pEU-E01-His-TEV-MCS-N3		0.3 mg/mL	10 μL	1	-20℃
5	pEU-E01-MCS-TEV-His-C1	C set	0.3 mg/mL	10 μL	1	-20℃
6	pEU-E01-MCS-TEV-His-C2		0.3 mg/mL	10 μL	1	-20℃
7	pEU-E01-MCS-TEV-His-C3		0.3 mg/mL	10 μL	1	-20℃
13	pEU-E01-GST-PS-MCS-N1	N set	0.3 mg/mL	10 μL	1	-20℃
14	pEU-E01-GST-PS-MCS-N2		0.3 mg/mL	10 μL	1	-20℃
15	pEU-E01-GST-PS-MCS-N3		0.3 mg/mL	10 μL	1	-20℃
16	pEU-E01-MCS-PS-GST(r)-C1	C set	0.3 mg/mL	10 μL	1	-20℃
17	pEU-E01-MCS-PS-GST(r)-C2		0.3 mg/mL	10 μL	1	-20℃
18	pEU-E01-DYKDDDDK-MCS-N1	N set	0.3 mg/mL	10 μL	1	-20℃
19	pEU-E01-DYKDDDDK-MCS-N2		0.3 mg/mL	10 μL	1	-20℃
20	pEU-E01-DYKDDDDK-MCS-N3		0.3 mg/mL	10 μL	1	-20℃
21	pEU-E01-DHFR (positive control) 1.0 mg		1.0 mg/mL	10 μL	1	-20℃

This products are produced under a strike quality management system
offering high-quality reagents including wheat germ extracts from wheat
obtained by natural farming in Japan.

 

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