iMatrix-332 is a recombinant human laminin-332 E8 fragment protein expressed in Chinese Hamster Ovary (CHO)-S cells. iMatrix-332 contains the integrin-binding site of the laminin-332 molecule. iMatrix-332 is useful for differentiation of iPSC and ESCs into corneal epithelial cells. iMatrix332 also supports the culture of the other cells adhering to laminin-332 such as keratinocytes.
Content:
Recombinant human laminin-332 E8 fragment protein in 20 mM phosphate buffer, 500 mM NaCl Protein concentration: 0.5 mg/mL Amount: 175 μg / 0.35 mL / tube Product No. 892 031 350 μg / 2 tubes Product No. 892 032 1,050 μg / 6 tubes Storage: Store at 2°C to 15°C, protect from light.
Expiration date:
The shelf life is 2 years from the date of manufacture. The expiration date is printed on the carton.
Methods of use:
By the following method iMatrix-332 can be coated onto a culture vessel. The optimum coating density may differ by cell-type, cell-line, medium selected, or purpose. Insufficient coating density may result in the detachment of cells and varied cell conditions while the excessive coating density may lead to difficulty in detaching cells for passage.
Coating protocol:
Determine the optimal coating density. 0.5 μg/cm2 is a standard but test between 0.1 and 1.5 μg/cm2.
1) Dilute iMatrix-332 with PBS(-). Use the diluted iMatrix-332 immediately. To coat with 0.5 μg/cm2 onto a 6-well plate with 9.6 cm2/well, dilute 9.6 μL of iMatrix-332 with 2 mL of PBS(-) per well.
2) Place the diluted iMatrix-332 into a culture vessel and incubate either at 37°C for 1 h, or at room temperature for 3 h, or at 4°C overnight.
3) Aspirate the coating solution. Then, immediately seed your cells. Do not allow the coated surface to dry.
