cDNA Library, S. cerevisiae , Log Phase
cDNA Library, S. cerevisiae, Log Phase
Cat. No. FNK-02-701
Store at -20°C
Size 500 ng
Quantity
500 ng (40 ng/ul, 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality
- Number of independent clones: 3.6 x 106
- Average insert size : longer than 1 kb
Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
Note
* This library is to be used only by the purchaser. It is not allowed to amplify and transfer the library to a third person.
* Related products: human tissue specific cDNA libraries and cDNA libraries of model organisms
References
Construction of this library is described in Supplementary data of Ref.3
- obori M et al ”Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library.” Genes Cells 3: 459-475 (1998) PMID: 9753427
- Tanaka S and Nojima H ”Nik1: a Nim1-like protein kinase of S. cerevisiae interacts with the Cdc28 complex and regulates cell cycle progression.” Genes Cells 1, 905-921 (1996) PMID: 9077450
- Tougan T, Okuzaki D, Nojima H. Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification. Nucleic. Acids Res., 36(15):e92, (2008) PMID:18603591
