Xyltech BOF-01 is a new basal culture medium which controls proliferation of human iPS/ES cells on feeder cell.
By using Xyltech BOF-01 instead of general basal medium such as DMEM/F12, proliferation of human iPS/ES cells is suppressed. These cells can be maintained with Xyltech BOF-01 for about 3 days (72 hours) without medium change under general culture condition (37℃, 5% CO2).
Cell growth can be resumed by replacing Xyltech BOF-01 by general basal medium.

Xyltech BOF-01 makes you free from medium change on a weekend!

Data
 

Fig.1 Phase images of colonies and alkaline phosphatase staining of human iPS cells (201B7 strain) before and after culture by Xyltech BOF-01.

Fig.2 Cell proliferation rates of human iPS cells in normal human ES/iPS growth medium and Xyltech BOF-01.
Fig.1 and 2 demonstrate that Xyltech BOF-01 surppresses human iPS cells proliferation and keeps them in undifferentiated state.


Fig.3 Expression of undifferentiation markers in human iPS cells (201B7 strain) after maintained in Xyltech BOF-01 for 3 days)
Fig.3 demonstrates that Xyltech BOF-01 maintained human iPS cells in undifferentiated state.


Fig.4 Differentiation of three germ layers (Teratoma assay). Differentiation ablity was tested by transplantation of human iPS cells (201B7 strain, maintained in Xyltech BOF-01 for 3 days) onto immunodeficient mouse.
Fig.4 demonstrates that human iPS cells cultured by Xyltech BOF-01 maintains pluripotency.


Fig.5 Colony morphology changes during culture by normal medium and Xyltech BOF-01.
Fig.5 shows that human iPS/ES cells may change its colony morphology during culture by Xyltech BOF-01 medium. Original morphology can be resumed after medium change to normal human iPS/ES cell medium.