Xyltech BOF-01, medium for human iPS/ES cells (100ml)

Product#: 87-280
$110.00
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Xyltech BOF-01, medium for human iPS/ES cells


Cat No: 87-280
 
Size: 100ml
Storage: 4°C

Description


A new basal medium for controlling iPS/ES proliferation. You are now free from medium change on weekends!

Xyltech BOF-01

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Xyltech BOF-01 is a new basal culture medium which controls proliferation of human iPS/ES cells on feeder cell.
By using Xyltech BOF-01 instead of general basal medium such as DMEM/F12, proliferation of human iPS/ES cells is suppressed. These cells can be maintained with Xyltech BOF-01 for about 3 days (72 hours) without medium change under general culture condition (37℃, 5% CO2).
Cell growth can be resumed by replacing Xyltech BOF-01 by general basal medium.

Xyltech BOF-01 makes you free from medium change on a weekend!


Features
 

  • No optimization is required
    • Just replace your regular basal medium by Xyltech BOF-01.
  • Proliferation is suppressed for 3 days (72 hours) without medium change
    • You can leave cells on weekends.
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Data
 
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Fig.1 Phase images of colonies and alkaline phosphatase staining of human iPS cells (201B7 strain) before and after culture by Xyltech BOF-01.

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Fig.2 Cell proliferation rates of human iPS cells in normal human ES/iPS growth medium and Xyltech BOF-01.
Fig.1 and 2 demonstrate that Xyltech BOF-01 surppresses human iPS cells proliferation and keeps them in undifferentiated state.

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Fig.3 Expression of undifferentiation markers in human iPS cells (201B7 strain) after maintained in Xyltech BOF-01 for 3 days)
Fig.3 demonstrates that Xyltech BOF-01 maintained human iPS cells in undifferentiated state.

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Fig.4 Differentiation of three germ layers (Teratoma assay). Differentiation ablity was tested by transplantation of human iPS cells (201B7 strain, maintained in Xyltech BOF-01 for 3 days) onto immunodeficient mouse.
Fig.4 demonstrates that human iPS cells cultured by Xyltech BOF-01 maintains pluripotency.

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Fig.5 Colony morphology changes during culture by normal medium and Xyltech BOF-01.
Fig.5 shows that human iPS/ES cells may change its colony morphology during culture by Xyltech BOF-01 medium. Original morphology can be resumed after medium change to normal human iPS/ES cell medium.



 
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