Viral DNA/RNA Isolation Kit (1x96 well)
Cat. No.: BMG-GD2811-00
Size: 1x96 well
Description:
EZgeneTM 96-Well blood viral DNA Kit provides an easy and reliable method for isolating total viral DNA/RNA from plasma, serum, whole blood, urine and cell culture supernatant in a 96-well format. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis C and HIV. The isolated DNA/RNA can be used for PCR, RT-PCR and other downstream applications.
Applications:
- Isolation of viral DNA and RNA from a wide range of biological sources, including:
Plasma
Serum
Saliva
Urine
Cell culture media
Nasopharyngeal and oropharyngeal swabs
Bronchoalveolar lavage (BAL)
Sputum
- Enables rapid processing of large sample numbers for viral detection and characterization
- Suitable for epidemiological studies and surveillance programs
- Preparation of viral nucleic acids for downstream diagnostic assays, particularly:
RT-PCR
qPCR
Real-time PCR
- Isolation of viral genomes for:
Next-Generation Sequencing (NGS)
Viral genome characterization
Mutation analysis
Phylogenetic studies
- Isolation of nucleic acids from various viruses, including:
Coronaviruses (e.g., SARS-CoV-2)
Influenza viruses
HIV
HCV
Enteroviruses
Flaviviruses
- Many 96-well kits are designed for use with automated liquid handling platforms, increasing throughput and reducing hands-on time
Features:
- Isolates high-quality viral DNA and RNA
- Yield: Up to 5 μg DNA and 10 μg RNA
- Size range of isolated nucleic acids: 50 nt to ~200 kb
- Uses a single buffer system for viral particle lysis and nucleic acid adsorption
- 96-well plate format for high-throughput processing
- Rapid processing time
- No organic denaturants or proteases used
- Elution with DNase/RNase-Free Water
- No post-purification DNase treatment required for most applications
- Compatible with automated liquid handling platforms for increased throughput
Reference:
Pichl, Lutz et al. “Magnetic bead technology in viral RNA and DNA extraction from plasma minipools.” Transfusion vol. 45,7 (2005): 1106-10. doi:10.1111/j.1537-2995.2005.04356.x
Lecamwasam, Ashani et al. “DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study.” PloS one vol. 13,2 e0190280. 20 Feb. 2018, doi:10.1371/journal.pone.0190280
