ResiQuant CHO HCP Elisa Kit 2G
Cat. No.: EC-CRH00-3022
Size: 96 Tests
Description
ResiQuant® CHO HCP Elisa Kit is used for quantitative detection of CHO host protein residues in the production process of products expressed and produced with CHO cell lines as host cells, so as to realize the control of CHO HCP residues.
Product Performance

Product Operation
Three step method: improve the sample applicability of the reagent kit, while amplifying the signal and improving sensitivity

Product Features
- Quantitative Range: 0.8-200 ng/mL
- Sensitivity: Lower limit of detection (LOD): 0.5 ng/mL, lower limit of quantitation(LOQ): 0.8 ng/mL
- Precision: Within the limit of quantification, the coefficient of variation of the sample concentration within the plate and between the plates is less than 20%
- Exclusivity: The concentration of CHO HCP measured in E.coli, 293T, Vero and other cell supernatants and non-CHO-expressing human IgG samples were all lower than the lower limit of detection
- Specificity: 84% antibody coverage (AAE-LC-MS).
Product Advantages
- Excellent performance
The product has a wide quantitative range, which can reach 0.8-200 ng/mL; the recovery rate of standard addition is qualified, meeting
the requirements of 70-130%.

- High antibody coverage
The coverage of antibodies detected by AAE-LC-MS was as high as 84%, including most of the CHO HCPs. Antibodies that could
recognize antigens accounted for 72/75 HCPs; high-risk HCPs accounted for 22/25.
- Complete traceability system
The calibrators and antibodies are independently prepared and developed, the raw materials are independently controllable, and the
traceability system of the kit is complete.
- Representative preparation process
CHO-S and CHO-K1 cell lines were selected to simulate the recombinant antibody preparation process, and the secretion supernatant
was used.
- Stable supply chain
The batch output is large, the delivery period is short, and the supply is stable. It supports at least 200,000 boxes of supply, which can
meet the needs of customers for continuous use.
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