UltraClean DEPC Treated TAE Buffer, 1X
Cat No.: DCP-TAEDEPC1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
DEPC Treated TAE Buffer (Tris-Acetate-EDTA) is a high-purity, nuclease-free electrophoresis buffer specifically formulated for sensitive nucleic acid applications. Treated with diethyl pyrocarbonate (DEPC), this buffer inactivates RNases and DNases, making it ideal for both DNA and RNA analysis where protection from enzymatic degradation is essential.
Applications:
- Agarose Gel Electrophoresis: Ideal for separation and analysis of DNA and RNA fragments, especially large genomic DNA (>1500 bp) and complex mixtures
- Polyacrylamide Gel Electrophoresis: Suitable for both DNA and RNA applications
- Native and Denaturing RNA Analysis: Provides a nuclease-free environment for sensitive RNA work
- Southern Blotting: Preferred buffer for gels used in Southern blot protocols, as acetate is less inhibitory than borate for downstream applications
- Gel Preparation: Can be used as both a running buffer and a gel preparation buffer
- Gel Purification: TAE buffer is favored for gel purification procedures due to its minimal interference with downstream enzymatic reactions
Advantages:
- Nuclease-Free: DEPC treatment ensures the buffer is free from RNase and DNase activity, providing maximum protection for RNA and DNA samples during electrophoresis
- High Purity: Manufactured using molecular biology-grade reagents and sterile filtration to guarantee the absence of contaminants
Composition:
| Ingredients |
Concentration |
| Tris base |
40 mM |
| EDTA |
1 mM |
| Glacial acetic acid |
20 mM |
| Water (DEPC-treated, nuclease-free) |
To volume |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 8.0
Storage and Shelf Life:
- Store at 4 °C.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
