Tris Triton-X Buffer
Cat No.: DCP-TTXB1X
Size: 100 mL & 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Tris-Triton Buffer is a gentle cell lysis buffer. It helps to maintain protein-protein interactions which makes it suitable for studying protein complexes. Triton X-100 can disrupt lipid-lipid and lipid-protein interactions in cellular membranes, solubilizing membrane proteins and releasing them from the membrane.The polyethylene oxide chains of Triton X-100 interact with the lipid bilayer, disrupting its integrity. This leads to the release of cytoskeletal proteins like actin, tubulin, and associated proteins from the insoluble cytoskeleton into the soluble fraction.The purpose of EDTA is to chelate divalent cations which are cofactors of many enzymes such as DNAses and proteases. NaCl provides an ionic environment that aids in protein solubilization and prevents non-specific binding. Tris HCl maintains a physiological pH, creating a mild environment suitable for protein extraction while minimizing denaturation. It can permeabilize and solubilize the plasma membrane, allowing access to the cytoskeletal network underneath.
Advantages:
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The Tris buffer component provides a mild, near-physiological pH environment that helps maintain the native conformation and activity of the extracted proteins.
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It is a versatile buffer compatible with various downstream applications like SDS-PAGE, Western blotting, immunoprecipitation, and enzyme assays involving the extracted proteins.
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Non-denaturing cell lysis: effective for lysing cells under non-denaturing conditions, preserving the native state of proteins.
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Protein extraction and solubilization: useful for extracting components of cell membranes and cytoplasmic proteins.
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Wide pH range: allows for the preparation of buffers with a wide pH range, from acidic to alkaline.
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Minimal interference: little interference with biochemical processes and doesn't precipitate with calcium, magnesium, or heavy metal ions.
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High buffering capacity: It provides strong buffering capacity, especially between pH 7.5-9.0, helping to stabilize the pH of reaction systems.
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Versatility: widely used in biochemistry, molecular biology, in vitro diagnostics, and other fields.
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Compatibility: It's inert to many enzyme reactions and has high solubility in water.
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Suitable for various techniques: It's used in procedures for multiple protein analysis methods.
Composition:
| Ingredients |
Concentration |
| Tris-HCl |
50 mM |
| NaCl |
150 mM |
| Triton |
1% |
| EDTA |
5 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
