Tris Borate EDTA Running Buffer [10X]
Cat No.: DCP-TBERB10X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Tris Borate EDTA Buffer (TBE) is commonly used in molecular biology techniques involving nucleic acids, especially in gel electrophoresis of DNA or RNA. Tris and Boric acid provide alkaline pH to keep nucleic acids deprotonated and soluble. EDTA protects nucleic acids from degradation by nucleases; its component chelates divalent cations like magnesium. EDTA’s low concentration allows the activity of useful DNA-modifying enzymes like restriction enzymes and DNA polymerases. This buffer creates an environment that enables the migration of negatively charged nucleic acids through the agarose or polyacrylamide gel matrix during electrophoresis. It is useful for separating short DNA or RNA fragments as it has a high buffering capacity compared to other buffers.
Advantages:
-
It is useful for separating short nucleic acid fragments during gel electrophoresis.
-
Its high buffering capacity allows for stable pH conditions during electrophoresis runs.
-
EDTA helps to protect the sample during electrophoresis.
-
Its slightly basic pH keeps nucleic acids deprotonated and soluble in the aqueous buffer solution.
Applications:
Composition:
| Ingredients |
Concentration |
| Tris base |
0.89 M |
| Boric acid |
0.89 M |
| EDTA |
0.02M |
Quality Control:
-
Appearance: Clear, Colorless Liquid.
-
pH: 8.3
-
Sterility: Filtered 0.1 micron Twice and 0.04 micron in a non-sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
-
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
-
Storage: 4 ℃
-
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
