Taq Premix (500 units)
Product#: FNK-02-100-5
$200.98
Availability:
Ships in 1-2 Weeks
Taq Premix
Cat No: FNK-02-100-5
Description
Taq Premix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions bacterial colonies and cDNA products.
Order Information
| Cat No. | Size | Link |
| FNK-02-100 | 100 units |  |
| FNK-02-100-5 | 500 units | |
Applications
- PCR
- Primer Extension
- Colony PCR
- High-Throughput PCR
Composition of PCR reaction Mixture (total 50μl)
| Taq Premix with Dye | 25 ul |
| Template | < 500 ng |
| Primer 1 | 0.2~1.0 uM (final conc.) |
| Primer 2 | 0.2~1.0 uM (final conc.) |
| Sterile Distilled Water | up to 50 ul |
One unit is defined as the amount of enzyme that can incorporate 10nmols of total dNTPs into an acid-insoluble material in 30 minutes at 74℃ when activated salmon sperm DNA was used as template / primer.
Quality Assurance
Greater than 95% purity as determined by SDS-PAGE (CBBstaining). The absence of endonuclease and exonucleases was confirmed.
PCR Product
PCR products have one A added at the 3'-terminus. Thus, the PCR product can be used directly for cloning into a T-vector. Additionally, it is possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.
PCR Test
Good amplification result was obtained in PCR reaction using λDNA as a template (Fig.1).
PCR condition
98℃ 10 sec
57℃ 30 sec
72℃ 8 min 25 cycles (2 min in the case of 2kb DNA)
Premix Composition
10 mM Tris-HCl, 50 mM MgCl2, 0.2 mM dNTPs, 5 % Glycerol, 0.08 % NP-40, 0.05 % Tween-20, 25 units/ml Taq DNA Polymerase, pH 8.6 @ 25℃
Storage
- 20℃ , Notes: Repeated freezing and thawing may decrease enzyme activity. Once thawed, aliquot into PCR tubes and store at -20℃. If you store this product at 4℃, please use it within 3 months.
