Recombinant Trypsin (Powder), 10MG

Recombinant Trypsin (Powder), 10MG

Cat. No.: SB-G3440-10MG
Size: 10MG


Description

Trypsin is a serine protease that specifically cleaves lysine and arginine C-terminal peptide bonds in proteins. When the amino acid following lysine and arginine is proline, the cleavage activity of trypsin decreases .

Recombinant Trypsin ( Powder ) of this product was expressed and purified in Pichia pastoris using trypsin gene from pig. It did not contain endogenous nuclease and other protease activities. The molecular weight was about 25 kDa, and its purity was single main band de tested by SDS -PAGE. Its effective temperature is 20-60°C, the optimum temperature is 35°C ; the effective pH range is 7-10, and the optimum pH is 8.5. It can replace the natural extraction of trypsin used in various biotechnology processes , such as : recombinant insulin production, cell culture, cell fermentation, protein enzymemolysis, recombinant trypsin in addition to enzyme activity than the natural extraction of trypsin, the purity is also higher, and no other protease activity.

Enzyme Activity Unit and Enzyme Activity : The Reaction Conditions Are 25 ° C, PH 8.0, and the Reaction System I S 3.2 Ml. An ENZYME ANITITITU U WAS DE Fined as an increase of 0.003 in the absorbance of the reaction system at 253 nm by Enzymatic hydrolysis of BAEE per minute. Enzyme activity ≥3800 U/mg (BAEE method). 

Inhibitors : Its activity is inhibited by serine protease inhibitors (such as PMSF), and heavy metal ions can also inhibit its enzyme activity .

Storage and transportation

Ship with wet ice; Store at - 20°C for 24 months. 

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