HemEx-TypeH is optimized for expansion of human HSC. It is necessary to add L-glutamine at a concentration of 4mM.
HemEx®-Type9AØ for mouse HSCs
HemEx®-TypeH for human HSCs
Reference:
HemEx-Type9AØ
Becker HJ, Ishida R, Wilkinson AC, Kimura T, Lee MSJ, Coban C, Ota Y, Tanaka Y, Roskamp M, Sano T, Tojo A, Kent DG, Yamazaki S. Controlling genetic heterogeneity in gene-edited hematopoietic stemcells by single-cell expansion. Cell Stem Cell. 2023 Jul 6;30(7):987-1000.e8. doi:10.1016/j.stem.2023.06.002. Epub 2023 Jun 28. PMID: 37385251; PMCID: PMC10338855.
HemEx-TypeH
Sakurai M, Ishitsuka K, Ito R, Wilkinson AC, Kimura T, Mizutani E, Nishikii H, Sudo K, Becker HJ, Takemoto H, Sano T, Kataoka K, Takahashi S, Nakamura Y, Kent DG, Iwama A, Chiba S, Okamoto S, Nakauchi H, Yamazaki S. Chemically defined cytokine-free expansion of human haematopoietic stem cells. Nature. 2023 Mar;615(7950):127-133. doi: 10.1038/s41586-023-05739-9. Epub 2023 Feb 22. PMID: 36813966.