Recombinant Tritirachium album Proteinase K(PROK) (Bulk Order, Price Inquiry)

Product#: CSB-AP361972TIQ
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   The product Sizes are 100 g / 1 kg (3-5 working days), 10 kg (1 month)
   Bulk Order, Prices are inquire


Recombinant Tritirachium album Proteinase K(PROK)
 

Description

Proteinase K is applied in the nucleic acid extraction kit and completely digest various nucleases and other proteins. It can prevent viral nucleic acid degradation and optimize extraction efficiency.


Product Details  

 
Purity Greater than 95% as determined by SDS-PAGE.
Activity
Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg.
 
Target Names PROK
Uniprot No. P06873
Alternative Names Endopeptidase K,Tritirachium alkaline proteinase
Species Tritirachium album (Engyodontium album)
Mol. Weight 28.9 kDa
Relevance
Proteinase K is an extracellular serine endoproteinase produced by Tritirachium album Limber.Proteinase K belongs to the peptidase family S8.lls predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked a-amino groups.Proteinase K is a broad-spectrum protease that functions at wide pH and temperatw-e ranges.The optimum pH and temperature for the activity of this protease were approximately pH 7.5-8.0 and 55°C, respectively. In addition,0.2-1 %sodium dodecyl sulfate (SDS) and 10mM carbamide can activate its activity. The combination of these unique features makes Proteinase K as an important tool in scientific research and molecular diagnosis. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR.ln nucleic acid vaccine and molecular diagnosis,it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples. 
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
 
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
 
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
 
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
 
Notes Repeated freezing and thawing is not recommended.
Description No Nucleic acid residual. No DNase residue. No RNase residue.
Datasheet & COA Please contact us to get it.
References "Proteinase K from Tritirachium album Limber. Characterization of the clu·omosomal ge ne and expression of the cDNA in Escherichia coli." 
Gunkel F.A., Gassen H.G. 
Eur. J. Biochem. 179: L 85-194(1989) 
 
 
Complete Degradation of Nuclease
CSB-AP361972TIQ_Fig1 Detect Nucleic acid residue by agarose gel electrophores CSB-AP361972TIQ_Fig2 Detect DNase residue by agarose gel electrophores CSB-AP361972TIQ_Fig3 Detect RNase residue by agarose gel electrophores
 
High Purity High Stability
CSB-AP361972TIQ-SDS.jpg Purity greater than 95% as determined by SDS-PAGE. CSB-AP361972TIQ-AC5.jpg Enzymetic activities were asaayed every month during one year. It indicates that proteinase K is stable and enzyme activity is maintained more than 95%.
 
High Activity
CSB-AP361972TIQ-AC4.jpg Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated > 30U/mg. CSB-AP361972TIQ-AC6.jpg Activity measurements from cusabio were compared to from three other vendors. Proteinase K from cusabio is better than other vendors.


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