Earle's Balanced Salt Solution w/ Phenol Red w/o Sodium bicarbonate: 1X
Cat No.: DCP-EBSSRB1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Phenol Red, [-] Sodium Bicarbonate
Description:
Earle's Balanced Salts Solution (EBSS) without sodium bicarbonate is a chemically defined, isotonic buffer solution optimized for maintaining cellular integrity during in vitro procedures. Designed for researchers requiring precise control over buffering systems, this formulation omits sodium bicarbonate to enhance storage stability while allowing users to adjust carbonate levels according to experimental needs. This solution provides essential inorganic ions and energy substrates for short-term cell maintenance across diverse applications.
Advantages:
- Physiological Compatibility: EBSS without sodium bicarbonate maintains intracellular and extracellular osmotic equilibrium (240 mOsm/kg H2O ± 5%), ensuring minimal stress during cell handling. The formulation includes calcium chloride, magnesium sulfate, potassium chloride, and sodium chloride at concentrations mirroring physiological conditions. Glucose (1.0 g/L) serves as an energy substrate, while phenol red acts as a pH indicator (yellow at pH ≤6.8, transitioning to pink at ≥8.2).
- Flexible Buffering System: Unlike standard EBSS, this formulation excludes sodium bicarbonate, making it ideal for applications where CO2-independent buffering is preferred. Researchers can customize buffering capacity by adding sodium bicarbonate (2.2 g/L recommended) or alternative buffers like HEPES, depending on experimental requirements.
Applications:
Cell Culture Maintenance
- Cell Washing: Removes residual enzymes, serum proteins, or metabolic byproducts prior to subculturing or analysis.
- Cell Transport: Maintains viability during transfers between laboratories or equipment.
- Dilution Medium: Safely dilutes cell suspensions for counting or seeding without altering osmotic pressure.
Composition:
| Ingredients |
mg/L |
| Calcium Chloride Dihydrate |
265.000 |
| Magnesium Sulfate Anhydrous |
97.720 |
| Sodium Phosphate Monobasic |
122.000 |
| Potassium Chloride |
400.000 |
| Sodium Chloride |
6800.000 |
| D-Glucose |
1000.000 |
| Phenol Red Sodium Salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.6
- Osmolality: Approximately 240 mOsm/kg H2O
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
