Recombinant Streptavidin (Freeze-Dried Powder), 5MG
Cat. No.: SB-G3430-5MG
Size: 5MG
Description
Recombinant Streptavidin is a recombinant biotin-binding protein derived from Streptomyces avidinii expressed by Escherichia coli, No his tag. Streptavidin and biotin have high affinity (Kd~10-15M) and low non-specific binding, so streptavidin-biotin system is widely used in various aspects of biological analysis. Streptavidin is a tetramer protein composed of four identical peptide chains with a mass of about 65kDa and can bind four biotin molecules. The recombinant streptavidin of this product has exactly the same properties as the natural streptavidin, and its purity is higher. It can not only be coupled with active substances such as enzyme and fluorescein, but also can be coupled with solid phase carriers such as magnetic beads and microspheres.
- Source: E. coli strain carrying Streptomyces avidinii avidin gene;
- Properties: white lyophilized powder
- Definition of enzyme activity: One unit of the enzyme binds 1 μg of biotin at room temperature.
- Purity and activity: purity ≥95% detected by SDS-PAGE; Protein activity ≥15 U/mg.
Storage and transportation
Transport in wet ice packs; store at -20°C, valid for 12 months.
Product Contents
| Component Number |
Component |
G3430-5MG |
| G3430-1 |
Recombinant Streptavidin |
5 mg |
| Product Manual |
1 copy |
Assay Protocol
Recombinant Streptavidin lyophilized powder resolution: Weigh appropriate amount of lyophilized powder, resolution with water or deionized water, 2-8℃ for short-term storage. It is recommended to freeze at -20℃ or lower after packaging, which can be stored for a long time and avoid repeated freezing and thawing.
Note:
1. For your safety and health, please wear safety glasses, gloves, or protective clothing.
