Recombinant RNase A (10 mg/mL), 200UL
Cat. No.: SB-G3405-200UL
Size: 200UL
Description
Recombinant RNase A is A Recombinant endoribonuclease that specifically attacks the 3' -ends of cytosine (C) or uracil (U) residues in single-stranded RNA and cleaves phosphate diester bonds with adjacent nucleotides. Recombinant RNase A has the highest cleavage activity for single stranded RNA and has activity in A variety of reaction conditions: At low salt concentration (0-100 mM NaCl), it can be used to cut single stranded RNA, double stranded RNA, and RNA-DNA hybridization RNA strands; at high salt concentration (≥300 mM NaCl), only single stranded RNA is specifically cleaved. This Recombinant RNase A contain His-tag.
- The main purpose: the recombinant RNase A was used to prepare DNA without host RNA; Can minimize the risk of pathogen introduction into the bioprocess; Other research experiments in molecular biology and cell biology.
- Feature: Specifically degraded RNA, purified by recombinant expression and free from contamination of animal origin, is rigorously controlled for contamination of non-specific nuclease and protease activity.
- Origin: Pancreas RNase A gene from Bovine pancreas, recombinant expression of Pichia pastoris.
- Definition of enzyme activity: At room temperature, the amount of enzyme required to increase the A286 value by 0.0146 per minute in a 1 mL reaction system was defined as 1 enzyme activity unit. Determination conditions: 100 mM Tris-acetate, 1 mM EDTA, 1 mM cyclic 2′, 3′-CMP, pH 6.5.
- Purity and concentration:The purity is ≥95% by SDS-PAGE, DNase-free, Proteinase-free, 10 mg/mL.
- Enzyme storage buffer: 50 mM Tris-HCl，50% Glycerol，pH 7.4@25℃.
Storage and Handling Conditions
Transport with wet ice; Store at -20℃, valid for 12 months.
Composition
| Component Number |
Component |
G3405-200UL |
| G3405-1 |
Recombinant RNase A |
200 μL |
| G3405-2 |
Dilution Buffer |
1 mL |
| Product Manual |
1 copy |
Note
1. To facilitate microdilution, dilute the Recombinant RNase A with an Dilution Buffer as desired.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
