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Rat Corticosteroid 11-beta-dehydrogenase Isozyme 1, HSD11B1 ELISA Kit
Product#: BTL-E1928Ra
$686.40
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Rat Corticosteroid 11-beta-dehydrogenase Isozyme 1, HSD11B1 ELISA Kit
Cat. No#: BTL-E1928Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 3.29ng/L
Detection range: 7-1500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Corticosteroid 11-Beta-Dehydrogenase Isozyme 1
SKU#.: ? BTL- |
E1928Ra |
Assay type |
Sandwich |
Size |
3.29ng/L |
Detection range |
7-1500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone (By similarity). Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity). |
UniProt accession |
31,883 |
GeneID |
25116 |
Synonyms |
11-beta-HSD1;11-beta-hydroxysteroid dehydrogenase 1;11-DH;Corticosteroid 11-beta-dehydrogenase isozyme 1;HSD11B1 |
Gene names |
HSD11B1 |
Research area |
Signal Transduction; Cancer; Metabolism |
Target protein |
HSD11B1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1928Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.275 | 2.184 | 2.142 |
| 2.094 | |||
| 400ng/L | 1.928 | 1.927 | 1.885 |
| 1.927 | |||
| 200ng/L | 1.179 | 1.16 | 1.118 |
| 1.141 | |||
| 100ng/L | 0.741 | 0.694 | 0.652 |
| 0.647 | |||
| 50ng/L | 0.402 | 0.412 | 0.369 |
| 0.421 | |||
| 0ng/L | 0.046 | 0.042 | 0 |
| 0.039 |
Precision
We measured random samples of E1928Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 103.7 | 6.21 | 6 |
| Intra-Assay | 2 | 18 | 299.5 | 14.43 | 4.8 |
| Intra-Assay | 3 | 18 | 155.3 | 8.26 | 5.3 |