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Human Corticosteroid 11 Beta Dehydrogenase Isozyme 1, HSD11B1 ELISA Kit
Product#: BTL-E5120Hu
$686.40
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Human Corticosteroid 11 Beta Dehydrogenase Isozyme 1, HSD11B1 ELISA Kit
Cat. No#: BTL-E5120Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 14.25ng/L
Detection range: 30-9000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Corticosteroid 11-beta-dehydrogenase isozyme 1
SKU#.: ? BTL- |
E5120Hu |
Assay type |
Sandwich |
Size |
14.25ng/L |
Detection range |
30-9000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
11β-Hydroxysteroid dehydrogenase type 1, also known as cortisone reductase, is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It belongs to the family of short-chain dehydrogenases. |
UniProt accession |
32,401 |
GeneID |
3290 |
Synonyms |
11-beta-HSD1;11-beta-hydroxysteroid dehydrogenase 1;11-DH;Corticosteroid 11-beta-dehydrogenase isozyme 1;CORTRD2;HDL;HSD11;HSD11B;HSD11B1;HSD11L;Short chain dehydrogenase/reductase family 26C member 1 |
Gene names |
HSD11B1 |
Research area |
Signal Transduction |
Target protein |
HSD11B1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5120Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4800ng/L | 2.233 | 2.167 | 2.12 |
| 2.101 | |||
| 2400ng/L | 1.184 | 1.226 | 1.179 |
| 1.267 | |||
| 1200ng/L | 0.856 | 0.855 | 0.808 |
| 0.854 | |||
| 600ng/L | 0.418 | 0.437 | 0.39 |
| 0.456 | |||
| 300ng/L | 0.267 | 0.245 | 0.199 |
| 0.224 | |||
| 0ng/L | 0.043 | 0.046 | 0 |
| 0.05 |
Precision
We measured random samples of E5120Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2520.07 | 90.65 | 3.6 |
| Intra-Assay | 2 | 18 | 1871.23 | 119.69 | 6.4 |
| Intra-Assay | 3 | 18 | 777.42 | 49.7 | 6.39 |