0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

FluxMPS™ RPMI 1640 Medium w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMI-PB1X
$34.10
DCP-RPMI-PB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o Sodium Pyruvate, Sodium Bicarbonate

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (2.0 g/L) Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-RPMI-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes, and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate.

  • Glucose source: 2000 mg/L (2.0 g/L) D-Glucose — the sole defined carbon source in this bicarbonate-free formulation
  • L-Glutamine included at 300 mg/L; glutathione (reduced, 1.0 mg/L) provides additional antioxidant/redox support for lymphocyte and hematopoietic cultures
  • Sodium bicarbonate and sodium pyruvate both excluded — CO₂-independent base intended for HEPES (15–25 mM) supplementation for pH control
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, with mycoplasma-retentive filtration applied at every stage
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Phenol red (5.3 mg/L) retained for visual pH monitoring; appearance is an orange-colored, clear solution
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • pH 7.4 (USP <791>); osmolality 230–270 mOsm/kg H₂O (USP <785>)
CAT. NO.
DCP-RPMI-PB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 w/o Sodium Pyruvate, Sodium Bicarbonate — 1X Liquid
  • Media familyRPMI 1640
  • Glucose2000 mg/L (2.0 g/L)
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that activate TLR4 and confound immune-cell assays. FluxMPS™ is built to reduce those risk factors through a validated, multi-stage filtration process.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Low-particulate media for suspension immune-cell OoC and flow cytometry workflows.

science

Immune cell–optimized formulation

RPMI 1640 contains glutathione (reduced) and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm, with tightly controlled trace-metal and organic-carbon (TOC) content — minimizing lot-to-lot variability introduced by feed-water quality.

shield

Batch-tested endotoxin control

Release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch — reduces the risk of LPS-driven TLR4 activation artefacts in T cell and NK cell assays.

analytics

Rich nutrient profile

RPMI's specialized nutrient set for demanding immune cell types, delivered through a low-particulate filtration process for OoC use.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-Retentive Filtration

    Retains particulates in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II — Second-Pass Protection

    A second, dedicated prefilter protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm and 0.04 µm mycoplasma-retentive filtration applied at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMI-PB1X RPMI 1640 quadruple-stage filtration system diagram showing 0.1 micron times 2 and 0.04 micron times 2 filtration stages for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-PB1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-PB1X — RPMI 1640 — delivers 0.04 µm filtered, Microfluidics Suitable media for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration is intended to reduce particulate load associated with TLR4 activation in immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where a DMEM-type formulation would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models built on microfluidic and microphysiological system (MPS) platforms.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

A batch-tested, low-endotoxin release specification (<0.05 EU/mL) is intended to reduce the risk of LPS-driven T cell activation artefacts in CAR-T manufacturing and TIL expansion protocols.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base for glycolysis stress testing and ¹³C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.

¹³C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Low-particulate media supports flow cytometry and confocal imaging workflows. A phenol red–free variant of this formulation is available on request for applications requiring minimal optical background.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available in 500 mL and 1000 mL pack sizes. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
Appearance Orange-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 39 components across 4 categories
Pack sizes 500 mL, 1000 mL
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm + 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Grade Microfluidics Suitable (0.04 µm final cut-off)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains reduced glutathione and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Phenol red sodium salt 34487-61-1 5.300
Custom formulation: Contact support@diagnocine.com for DCP-RPMI-PB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm with controlled trace-metal and TOC content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-RPMI-PB1X lot at support@diagnocine.com.
Product Comparison

How DCP-RPMI-PB1X compares

FluxMPS™ DCP-RPMI-PB1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMI-PB1X (FluxMPS™) Conventional RPMI 1640
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
RPMI 1640 without Sodium Pyruvate and Sodium Bicarbonate — CO₂-free with defined secondary carbon check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel Fixed catalog only cancel Fixed catalog only

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-PB1X — RPMI 1640.

Yes. DCP-RPMI-PB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low-particulate media for MPS, OoC, and LoC platforms. RPMI 1640 with mycoplasma-retentive filtration is suited to immune-cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
 
This formulation is CO₂-independent (add HEPES 15–25 mM) with a researcher-defined secondary carbon source; L-Glutamine is already included at 300 mg/L. Add sodium pyruvate fresh (typically 1 mM) if your cell type requires it. This design is intended for atmospheric immune cell cultures where pyruvate concentration and buffering are experimental variables you control.
No. This formulation is bicarbonate-free and CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering under atmospheric conditions.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter — never 0.04 µm, which retains IgM, VLDL, and much of the lipid/lipoprotein fraction of serum. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMI-PB1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch. For immune cells, endotoxin activates TLR4/NF-κB signaling, inducing cytokine release and altering lymphocyte activation state independently of experimental conditions, which is why a low, batch-tested specification matters for immune assays.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  9. Poznansky MC, et al. Efficient generation of human T cells from a tissue-engineered thymic organoid. Nat Biotechnol. 2000;18:729–734. doi:10.1038/77288

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X