What makes the filtration process superior to standard 0.22 micron filtered media?

FluxMPS uses four sequential filters - 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II - reaching a 0.04 micron final cut-off, five times finer than the 0.22 micron membranes used in conventional sterile filtration.

FluxMPS RPMI Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Q: Can DCP-RPMIH-QPB1X be supplemented with serum, growth factors, or other components?

Yes. FBS (typically 5-10 percent), serum-free supplements, growth factors, or antibiotics can be added as required. Filter serum-containing or protein-containing additions through a 0.2 micron low-protein-binding PES or PVDF membrane; use 0.1 micron only for defined, protein-free additions. Never filter serum through 0.04 micron. Contact support@diagnocine.com for custom co-formulation.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. Full CoA per lot covers appearance, pH (USP less than 791 greater than), osmolality (USP less than 785 greater than), sterility (USP less than 71 greater than), endotoxin (USP less than 85 greater than), mycoplasma filtration status, particulate count (USP less than 788 greater than Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Phenol Red HEPES (25 mM) Calcium Magnesium Glucose (2.0 g/L) L-Glutamine Sodium Pyruvate Sodium Bicarbonate

FluxMPS™ DCP-RPMIH-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic, lymphocyte, and immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms.^[1,3] A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides bicarbonate-free, CO₂-independent pH buffering. Formulation: [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

HEPES-buffered (25 mM, pKa 7.3 at 37°C), bicarbonate-free formulation for CO₂-independent pH control during open-air handling and flow cytometry preparation
Glucose 2.0 g/L (2000 mg/L) as the sole defined carbon source; L-glutamine, sodium pyruvate, and sodium bicarbonate withheld so each can be titrated independently for metabolic flux studies
Reduced glutathione (1.0 mg/L) included for redox support in lymphocyte and hematopoietic cell culture
Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — see Purity Architecture below
Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
Contains phenol red sodium salt (5.3 mg/L); a phenol-red-free variant is available on request for fluorescence-sensitive assays
Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
39 formulation components released with full raw-material CAS traceability; custom pH, salts, and nutrient modifications available on request

CAT. NO.

DCP-RPMIH-QPB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 + 25mM HEPES — 1X Liquid

Formulation[+] Phenol Red, [+] HEPES, [+] Ca, [+] Mg, [+] Glucose | [-] Gln, [-] Pyruvate, [-] NaHCO₃
Glucose2000 mg/L (2.0 g/L)
HEPES25 mM, pKa 7.3 at 37°C
AppearanceOrange-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)230–270 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered RPMI passes subvisible particulates, mycoplasma in the 0.2–0.3 µm range, and endotoxin fragments that can confound sensitive lymphocyte and immune-cell assays. FluxMPS™ RPMI 1640 + 25mM HEPES is built to reduce these variables.^[1]

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate compliance provide particulate-clean media for suspension immune cell OoC, flow cytometry, and lymphocyte perfusion chips.

target

Total metabolic control

L-glutamine, sodium pyruvate, and sodium bicarbonate are withheld so glucose (2.0 g/L), amino acids, and buffering can each be titrated independently for immune-cell metabolic flux and Warburg-effect studies under HEPES-buffered, CO₂-independent conditions.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) feed stock supports low trace-metal and organic-carbon (TOC) background for sensitive lymphocyte and hematopoietic cell culture.

visibility

Low background for imaging

Quadruple-stage 0.04 µm filtration reduces particulate background for confocal imaging, biosensor, and flow cytometry channels. This formulation contains phenol red sodium salt (5.3 mg/L); a phenol-red-free variant is available on request for fluorescence-sensitive assays.

science

Rich, stable nutrient profile

RPMI 1640's amino acid and vitamin base, including reduced glutathione (1.0 mg/L) for redox support, is manufactured in micro-batches with full lot-to-lot CAS traceability across all 39 formulation components.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs — reach a 0.04 µm final cut-off prior to aseptic fill.^[4]

1

0.1 µm Prefiltration I

Removes large aggregates and cell debris; protects the first 0.04 µm final-filter cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter, including the 0.2–0.3 µm mycoplasma size range.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04

µm final pore size — sub-mycoplasma polishing

4

Filtration passes (0.1 µm ×2 + 0.04 µm ×2)

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled via 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every stage (not tested per lot; typical mycoplasma diameter 0.2–0.3 µm).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS RPMI 1640 with 25mM HEPES (DCP-RPMIH-QPB1X) quadruple-stage filtration system diagram showing 0.1 micron times 2 and 0.04 micron times 2 stages for organ-on-a-chip and microfluidic cell culture applications - Diagnocine — **Figure 1.** FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-QPB1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-QPB1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable purity for hematopoietic cell and related OoC applications.^[2,3]

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated perfusion and robotic culture systems.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off of this product
Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration reduces particulate load that can confound immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based formulations would alter proliferation and signaling.

JurkatRajiK562HL-60

Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models on microphysiological system (MPS) platforms.^[3]

Tumor-immune chipLymph node OoCMPS

Immunotherapy

CAR-T & TIL Expansion

Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Glutamine-, pyruvate-, and bicarbonate-free RPMI base supports independently titrated glycolysis studies and ¹³C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium; this formulation contains phenol red.

¹³C tracingGlycolysisLC-MS metabolomics

Live-Cell Imaging

Flow Cytometry & Confocal

Reduced particulate background from quadruple-stage 0.04 µm filtration supports flow cytometry and confocal imaging workflows; a phenol-red-free variant is available for fluorescence-sensitive channels.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) \| [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance	Orange-colored, clear solution
Glucose	2000 mg/L (2.0 g/L)
HEPES	25 mM (pKa 7.3 at 37°C)
L-Glutamine	None / Not added
Sodium Pyruvate	None / Not added
Sodium Bicarbonate	None / Not added
Phenol Red	Present, 5.3 mg/L (phenol-red-free variant available on request)
pH USP <791>	7.4
Osmolality USP <785>	230–270 mOsm/kg H₂O
Total ingredients	39 components across 4 categories

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification)
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, protect from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent — bicarbonate-free, 25 mM HEPES-buffered formulation
Available sizes	500 mL, 1000 mL

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 39 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others), verified per lot with CAS numbers for full raw-material traceability.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6000.000
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.00
Pyridoxine hydrochloride	58-56-0	1.00
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
i-Inositol	87-89-8	35.000
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
HEPES	7365-45-9	5958.00
Phenol red sodium salt	34487-61-1	5.300

Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-QPB1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train reaching a 0.04 µm final cut-off prior to aseptic fill.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMIH-QPB1X compares

FluxMPS™ DCP-RPMIH-QPB1X vs. conventional 0.22 µm-filtered RPMI formulations.

Parameter	DCP-RPMIH-QPB1X (FluxMPS™)	Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
Grade	Microfluidics Suitable	Not specified	Not specified
Formulation — HEPES-only, no glutamine, no pyruvate, no bicarbonate	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier filtration	check_circle Yes (0.1 / 0.04 µm)	cancel No	cancel No
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle Yes — Microfluidics Suitable (0.04 µm)	cancel Risk of clogging	cancel Risk of clogging
Custom formulation available	check_circle Yes	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-QPB1X — RPMI 1640 + 25mM HEPES.

Is DCP-RPMIH-QPB1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMIH-QPB1X is processed through a quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, making it Microfluidics Suitable for immune-cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models on microphysiological system (MPS) platforms.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.

Why are L-glutamine, sodium pyruvate, and sodium bicarbonate excluded, and how do I supplement for my cell type?expand_more

This HEPES-only, bicarbonate-free formulation lets glutamine, pyruvate, and buffering be titrated independently of the base medium, giving maximum flexibility for immune-cell metabolic flux analysis in CO₂-independent conditions. Add L-glutamine or a stable dipeptide substitute, sodium pyruvate, and/or sodium bicarbonate as required for your specific cell line and assay format. Contact support@diagnocine.com for a pre-supplemented custom formulation.

Does this medium require a CO₂ incubator?expand_more

No. This formulation is bicarbonate-free and HEPES-buffered (25 mM, pKa 7.3 at 37°C), providing CO₂-independent pH stability for open-air handling and non-CO₂ incubation. Validate pH stability under your specific culture conditions and cell line.

Can DCP-RPMIH-QPB1X be supplemented with serum, growth factors, or other components?expand_more

Yes. FBS (typically 5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane; use 0.1 µm only for defined, protein-free additions. Never filter serum through 0.04 µm — that pore size retains IgM, VLDL, chylomicrons, and much of the serum lipoprotein fraction. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Low endotoxin levels reduce the risk of LPS-driven TLR4/NF-κB activation, which can confound lymphocyte proliferation and cytokine assays.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune-cell culture and OoC applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ RPMI Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid