What makes the filtration process different from standard 0.22 micron filtered media?

FluxMPS runs four sequential filtration passes - 0.1 micron prefiltration I, 0.04 micron final filtration I, 0.1 micron prefiltration II, and 0.04 micron final filtration II (polish) - reaching a 0.04 micron final cut-off, five times finer than the 0.22 micron membranes used for conventional sterile filtration, with a mycoplasma-retentive 0.1 micron stage built into the train.

FluxMPS RPMI 1640 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Q: Does DCP-RPMIH-QP1X require a CO2 incubator?

5 percent CO2 is recommended for bicarbonate buffering; 25 mM HEPES provides supplemental pH stability during open-bench handling.

Q: Can DCP-RPMIH-QP1X be supplemented with serum, growth factors, or other components?

Yes. Add FBS (5-10 percent), serum-free supplements, or growth factors as required. Filter serum-containing additions through a 0.2 micron low-protein-binding PES or PVDF membrane, never a 0.04 micron membrane. Contact support@diagnocine.com for custom co-formulation.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. Full CoA per batch covers appearance, pH (USP less than 791 greater than), osmolality (USP less than 785 greater than), sterility (USP less than 71 greater than), endotoxin (USP less than 85 greater than), mycoplasma-retentive filtration status, particulate count (USP less than 788 greater than Method 1), and raw-material traceability. Request at support@diagnocine.com.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES (w/o L-Glutamine & Sodium Pyruvate)

Sodium Bicarbonate Phenol Red 25mM HEPES Calcium Magnesium Glucose L-Glutamine Sodium Pyruvate

FluxMPS™ DCP-RPMIH-QP1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic and lymphocyte cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) supplements bicarbonate buffering for pH stability during open-bench handling and sample prep. Formulation: [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] L-Glutamine, [-] Sodium Pyruvate.

Glucose source: 2000 mg/L (2.0 g/L) D-Glucose for a defined carbon input
25 mM HEPES (pKa 7.3 at 37°C) supplements bicarbonate buffering for pH stability during open-bench handling
Glutathione (reduced, 1.0 mg/L) included — antioxidant support for redox-sensitive lymphocyte and hematopoietic cultures
No L-Glutamine or Sodium Pyruvate — formulate your own nitrogen/carbon supplementation regimen
Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off; endotoxin release specification < 0.05 EU/mL
Phenol red present (5.3 mg/L) for visual pH monitoring; a phenol-red-free variant is available on request for imaging-sensitive assays
Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
Custom pH, salts, HEPES, and nutrient composition available on request

CAT. NO.

DCP-RPMIH-QP1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 + 25mM HEPES — 1X Liquid

Media familyRPMI 1640 + 25mM HEPES
Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] L-Glutamine, [-] Sodium Pyruvate
Glucose2000 mg/L (2.0 g/L)
HEPES25 mM, pKa 7.3 at 37°C
pH (USP <791>)7.4
Osmolality (USP <785>)280 - 320 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2-8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes subvisible particulates and lacks a dedicated mycoplasma-retentive stage, both of which can confound sensitive lymphocyte and hematopoietic assays. FluxMPS™ is built to reduce those risks at the filtration stage.

filter_alt

Microchannel-safe, low-endotoxin purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance and a < 0.05 EU/mL endotoxin release specification — formulated to reduce the risk of particulate and LPS-driven activation artefacts in immune cell assays.

target

Dual metabolic & pH control

No L-glutamine or sodium pyruvate — add your own nitrogen and carbon sources at the concentration your assay requires. 25 mM HEPES supplements bicarbonate buffering for pH-stable handling outside the incubator.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm) to minimize ionic and trace-metal contaminants carried into the finished medium.

visibility

Low background for imaging

The 0.04 µm final filtration reduces particulate baseline for confocal and biosensor-based workflows. Note this formulation contains phenol red (5.3 mg/L); a phenol-red-free variant is available on request for autofluorescence-sensitive imaging modalities.

science

Immune cell–optimized formulation

RPMI 1640 base contains glutathione (reduced, antioxidant support) and the standard balanced amino acid and vitamin profile used for lymphocyte and hematopoietic cell culture.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two paired prefilter/final-filter cycles — reaching a 0.04 µm final cut-off under aseptic fill conditions.

1

0.1 µm Prefiltration I — Large Particulate Removal

Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.
2

0.04 µm Final Filtration I — Mycoplasma-Retentive Pass

First 0.04 µm pass; a mycoplasma-retentive grade of filtration not present in standard 0.22 µm processing.
3

0.1 µm Prefiltration II — Second Prefilter

Dedicated second prefilter protecting the second 0.04 µm final filter cartridge.
4

0.04 µm Final Filtration II — Polish

Second 0.04 µm pass; final polishing filtration ahead of aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration, with a mycoplasma-retentive 0.1 µm stage built into every production run.

0.04

µm final pore size across 4 filtration passes

5.5×

Finer final cut-off vs. 0.22 µm conventional filtration

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration built into the production train (not tested per lot by USP <63>).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS DCP-RPMIH-QP1X RPMI 1640 25mM HEPES quadruple-stage 0.1 micron x2 plus 0.04 micron x2 filtration system for organ-on-a-chip and microfluidic cell culture, Diagnocine — **Figure 1.** FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-QP1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-QP1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered purity for hematopoietic cells, lymphocytes, and related microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Immune Cell OoC & MPS

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune microphysiological systems, and lymph node-on-chip models.

OoCToCBoCLoCMPS

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes; 0.04 µm filtration reduces particulate exposure in sensitive immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based formulations would alter proliferation and signaling behavior.

JurkatRajiK562HL-60

Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation artefacts.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium. Suited to ¹³C isotope tracing and LC-MS–based metabolic flux analysis of lymphocyte activation states.

¹³C tracingLC-MS fluxGlycolysis

Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate baseline for flow cytometry and confocal imaging of immune cell models; a phenol-red-free variant is available for autofluorescence-sensitive channels.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose \| [-] L-Glutamine, [-] Sodium Pyruvate
Appearance	Red-colored, clear solution (phenol red present)
Glucose	2000 mg/L (2.0 g/L)
HEPES	25 mM (pKa 7.3 at 37°C)
pH USP <791>	7.4
Osmolality USP <785>	280 - 320 mOsm/kg H₂O
Total ingredients	40 components across 4 formulation categories

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1	NMT 25/mL
Particulate ≥25 µm USP <788> M1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2-8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ recommended for bicarbonate buffering; 25 mM HEPES provides supplemental pH stability during open-bench handling

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains glutathione (reduced, antioxidant) and the standard balanced RPMI 1640 amino acid and vitamin profile used for lymphocyte and hematopoietic cell culture.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.000
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
HEPES	7365-45-9	5958.000
Phenol red sodium salt	34487-61-1	5.300
i-Inositol	87-89-8	35.000

Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-QP1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — minimizes ionic and trace-metal contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280 - 320 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMIH-QP1X compares

FluxMPS™ DCP-RPMIH-QP1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter	DCP-RPMIH-QP1X (FluxMPS™)	Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
Grade	Microfluidics Suitable	Not specified	Not specified
Formulation: no Glutamine, no Pyruvate; dual bicarbonate + HEPES buffering	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration passes	4 (Quadruple-stage)	1	1
Mycoplasma-retentive filtration	check_circle Yes (0.1 µm stage)	cancel No	cancel No
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested	check_circle Yes	cancel Not specified	cancel Not specified
Water quality	Type 1, 18.2 MΩ·cm	Not specified	Not specified
Manufacturing QMS	ISO 13485:2016	Not specified	Not specified
Microfluidic channel compatibility	check_circle Microfluidics Suitable	cancel Risk of clogging	cancel Risk of clogging
Custom formulation available	check_circle Yes	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-QP1X — RPMI 1640 + 25mM HEPES.

Is DCP-RPMIH-QP1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMIH-QP1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size with a built-in 0.1 µm mycoplasma-retentive stage. It is Microfluidics Suitable and used in immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.

What makes the filtration process different from standard 0.22 µm filtered media?expand_more

FluxMPS™ runs four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration, with a mycoplasma-retentive 0.1 µm stage built into the train.

Why are L-glutamine and sodium pyruvate excluded, and how do I supplement?expand_more

Both are omitted so you can supplement fresh L-glutamine (or a stabilized dipeptide alternative) and sodium pyruvate at the concentration your protocol requires. Note this formulation contains sodium bicarbonate and phenol red and is therefore not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; it is well suited to standard flux and proliferation assays that do not require the XF platform.

Does DCP-RPMIH-QP1X require a CO₂ incubator?expand_more

5% CO₂ is recommended for bicarbonate buffering; 25 mM HEPES provides supplemental pH stability during open-bench handling.

Can DCP-RPMIH-QP1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Add FBS (5-10%), serum-free supplements, or growth factors as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane — never a 0.04 µm membrane, which will strip serum of the proteins and lipoproteins it needs to function. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and organ-on-a-chip applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Rothbauer M, et al. Recent advances in microfluidic technologies for cell-to-cell interaction studies. Lab Chip. 2018;18:249–270. doi:10.1039/c7lc00815e
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-chip: a fast track for engineered human tissues in drug development. Cell Stem Cell. 2018;22:310–324. doi:10.1016/j.stem.2018.02.011
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid