What makes the filtration process superior to standard 0.22 micron filtered media?

FluxMPS uses a Quadruple-stage system (0.1 micron x2 + 0.04 micron x2), two paired prefilter and final-filter stages reaching a 0.04 micron final pore size, well below the 0.22 micron used in standard filtration, with mycoplasma-retentive filtration at each 0.1 micron stage.

Does this medium require a CO2 incubator?

Yes. This formulation contains sodium bicarbonate (2000 mg/L) as its primary buffer, formulated to maintain pH 7.4 at 5% CO2. The additional 25 mM HEPES provides supplemental buffering for brief periods outside the incubator.

FluxMPS RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Q: Is DCP-RPMIGH-QPR1X suitable for organ-on-a-chip (OoC) applications?

Yes. DCP-RPMIGH-QPR1X is Microfluidics Suitable and engineered for MPS and OoC platforms. The 0.04 micron final filtration reduces the risk of microfluidic channel fouling, making it suitable for OoC, ToC, BoC, and LoC applications sensitive to particulate contamination.

Q: Why does this formulation exclude L-glutamine, sodium pyruvate, and phenol red, and how do I supplement for my cell type?

L-glutamine is withheld because it degrades in liquid solution over time; add fresh L-glutamine or a stabilized dipeptide substitute at the concentration your protocol specifies. Sodium pyruvate is withheld so it can be added independently for assays that depend on it. Phenol red is withheld to eliminate background absorbance and fluorescence for optical assays and imaging on-chip.

Q: Can DCP-RPMIGH-QPR1X be supplemented with serum, growth factors, or other components?

Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, or growth factors as required. Filter serum-containing supplements through a 0.2 micron low-protein-binding PES or PVDF membrane; do not use a 0.04 micron membrane for serum.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. CoA is available per lot upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, mycoplasma filtration statement, USP chapter 788 particulate data, appearance, and full raw material lot traceability.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Sodium Bicarbonate 25 mM HEPES Calcium Magnesium High Glucose (4500 mg/L) L-Glutamine Phenol Red Sodium Pyruvate

FluxMPS™ DCP-RPMIGH-QPR1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 formulation engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final polish well beyond the reach of conventional 0.22 µm–filtered media.

High-glucose (4500 mg/L) RPMI 1640 base with 25 mM HEPES supplemental buffering
Formulated without L-glutamine, sodium pyruvate, and phenol red for custom supplementation at time of use
Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
Endotoxin release specification < 0.05 EU/mL per manufacturing batch (LAL, USP <85>)
Sodium bicarbonate (2000 mg/L) buffer system formulated to hold pH 7.4 at 5% CO₂
Manufactured under an ISO 13485:2016 quality management system with full lot traceability
38 verified ingredients per lot with full CAS traceability
Microfluidics Suitable — engineered for OoC, ToC, BoC and microfluidic channel applications

CAT. NO.

DCP-RPMIGH-QPR1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Formulation[+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose, [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
Glucose4500 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)Contact for specification
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened
ShippingCold pack, 2–8°C maintained in transit
Sizes500 mL, 1000 mL

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms and subvisible particulates that a microfluidic channel or a chip-based sensor cannot tolerate.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Low-particulate media for OoC microfluidic chips.

target

Total metabolic control

High glucose (4500 mg/L) with L-glutamine, sodium pyruvate, and phenol red withheld so you control the carbon source, energy substrate, and imaging background at time of use.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm resistivity, with trace-metal and total organic carbon (TOC) control at the point of manufacture.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal, biosensor, and live-cell imaging applications on-chip.

science

Rich, stable nutrient profile

38 verified ingredients per lot. Full CAS traceability. Micro-batch precision manufacturing.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter-plus-final-filter pairs — reaching a final 0.04 µm polish.

1

0.1 µm Prefiltration I

Removes large aggregates and debris; protects the first 0.04 µm cartridge and extends its service life.
2

0.04 µm Final filtration I

Provides mycoplasma-retentive filtration (mycoplasma range 0.2–0.3 µm) beyond the reach of standard 0.22 µm membranes. First sub-mycoplasma polishing pass.
3

0.1 µm Prefiltration II

A second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2’s effluent, but redundant protection for Stage 4.
4

0.04 µm Final filtration II — Polish

Ultimate sub-mycoplasma polish immediately prior to aseptic fill and finish. Final product QC release gate.

Performance vs. conventional media

FluxMPS™ DCP-RPMIGH-QPR1X is processed through two paired prefilter + final-filter stages (0.1 µm ×2 + 0.04 µm ×2), reaching a final pore size well below the 0.22 µm used in conventional cell culture media.

4

Serial filtration passes, two paired prefilter + final-filter stages

0.04

µm final pore size — sub-mycoplasma polishing

Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: controlled by 0.1 µm mycoplasma-retentive filtration; not tested per lot.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS™ DCP-RPMIGH-QPR1X RPMI 1640 High Glucose HEPES medium ? Quadruple-stage filtration (0.1 μm ×2 + 0.04 μm ×2) for organ-on-a-chip and microfluidic cell culture applications | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-RPMIGH-QPR1X.
© Diagnocine® — DCP-RPMIGH-QPR1X

Applications

OoC and MPS Applications

FluxMPS™ DCP-RPMIGH-QPR1X delivers Microfluidics Suitable purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

A separate 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems requiring the tighter cut-off.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip where low-particulate media reduces channel fouling.

OoCToCBoCLoCMPS

Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose RPMI base supports NCI-60 cancer lines and Warburg-effect metabolic studies with user-defined carbon-source control.

MCF-7MDA-MB-231HeLaJurkat

Stem Cell Biology

iPSC-Derived Models

Ultra-clean base for iPSC differentiation protocols requiring defined, low-particulate media.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Low-particulate formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes

Metabolomics

Metabolic Flux Analysis

Defined formulation for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium; this formulation contains sodium bicarbonate.

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate background for confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose, [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
Appearance	Pale yellow to colorless, clear solution (phenol red-free)
Total ingredients	38
pH USP <791>	7.4
Osmolality USP <785>	Contact for specification
Glucose	4500.000 mg/L

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (per manufacturing batch)
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std. ISO	ISO 13485:2016
Fill environment	Aseptic fill & finish

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, protect from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack, 2–8°C maintained in transit
CO₂ requirement	5% CO₂ (derived from 2000 mg/L sodium bicarbonate to hold pH 7.4); 25 mM HEPES provides supplemental buffering

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid — 38 ingredients verified per lot with CAS numbers for full raw-material traceability.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.00
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	4500.000
Glutathione reduced	70-18-8	1.000
HEPES	7365-45-9	5958.000
i-Inositol	87-89-8	35.000

Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.

Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-RPMIGH-QPR1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity feedwater with trace-metal and total organic carbon (TOC) control at the manufacturing source.

biotech

Quadruple-Stage Filtration

Two paired 0.1 µm prefilter + 0.04 µm final-filter stages, followed by aseptic fill & finish immediately after the final polish.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

LAL-based endotoxin test per manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate analysis. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMIGH-QPR1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter	DCP-RPMIGH-QPR1X (FluxMPS™)	Conventional 0.22 µm Filtered	Standard Alternative
Grade	Microfluidics Suitable (0.04 µm)	Standard reagent grade	Standard reagent grade
Formulation	[+] Sodium Bicarbonate, [+] 25mM HEPES, [+] High Glucose, [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate	Standard RPMI 1640	Standard RPMI 1640
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 stages	1 stage	1–2 stages
Mycoplasma-retentive filtration	check_circle	cancel	cancel
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance	check_circle	cancel	cancel
Water quality	Type 1, 18.2 MΩ·cm	Type 2 typical	Type 2 typical
Manufacturing QMS	ISO 13485:2016	ISO 9001 typical	Variable
Microfluidic channel compatibility	check_circle	cancel	cancel
Custom formulation	check_circle	cancel	Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH-QPR1X and its use in OoC and MPS applications.

Is DCP-RPMIGH-QPR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMIGH-QPR1X is Microfluidics Suitable and engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces the risk of microfluidic channel fouling, making it suitable for OoC, ToC, BoC, and LoC applications sensitive to particulate contamination.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

FluxMPS uses a Quadruple-stage system (0.1 µm ×2 + 0.04 µm ×2) — two paired prefilter and final-filter stages reaching a 0.04 µm final pore size, well below the 0.22 µm used in standard filtration, with mycoplasma-retentive filtration at each 0.1 µm stage.

Why does this formulation exclude L-glutamine, sodium pyruvate, and phenol red, and how do I supplement for my cell type?expand_more

L-glutamine is withheld because it degrades in liquid solution over time; add fresh L-glutamine (or a stabilized dipeptide substitute such as GlutaMAX) at the concentration your protocol specifies. Sodium pyruvate is withheld so you can add it independently for assays that depend on it as an energy substrate. Phenol red is withheld to eliminate background absorbance/fluorescence for optical assays, biosensors, and imaging on-chip.

Does this medium require a CO₂ incubator?expand_more

Yes. This formulation contains sodium bicarbonate (2000 mg/L) as its primary buffer, formulated to maintain pH 7.4 at 5% CO₂. The additional 25 mM HEPES provides supplemental buffering capacity for brief periods outside the incubator. Incubate at 37°C with 5% CO₂ unless your protocol specifies otherwise.

Can DCP-RPMIGH-QPR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. This is a basal medium formulation. Supplement with FBS, serum replacements (e.g., B27, N2), or growth factors as required by your cell type and protocol. Filter serum-containing and protein-containing supplements through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for serum, as it will strip serum of the proteins and lipoproteins needed for cell growth.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by the Limulus Amebocyte Lysate (LAL) Bacterial Endotoxins Test per USP <85> and must meet the release specification of < 0.05 EU/mL before release. A Certificate of Analysis is available on request.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. CoA is available per lot upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin (release specification and LAL method), sterility, mycoplasma filtration statement, USP <788> particulate data, appearance, and full raw material lot traceability.

Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC/MPS applications and FluxMPS™ ultra-filtered cell culture media.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Engineered where standard media fails

Microchannel-safe purity

Total metabolic control

Ultrapure-grade water

Low background for imaging

Rich, stable nutrient profile

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

OoC and MPS Applications

Automated Bioreactors & Robotics

Micro Physiological System (MPS) & Chip

Cancer Cell Lines & Metabolic Research

iPSC-Derived Models

Endothelial & Primary Cells

Metabolic Flux Analysis

Microscopy & Optical Sensing

Analytical release specifications

Full composition (mg/L)

ISO 13485 Manufacturing & Compliance

ISO 13485:2016 QMS

Ultrapure Type 1 Water

Quadruple-Stage Filtration

Micro-Batch Precision

Endotoxin USP <85> BET

Particulate USP <788> Method 1

Osmolality USP <785>

Documentation / CoA

How DCP-RPMIGH-QPR1X compares

Frequently asked questions

Supporting literature

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