Protease Inhibitor Cocktail with Tris Triton-X Buffer
Cat. No. DCP-PICTTX1X
Size: 10x10 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
This Ready-to-use combination of a protease inhibitor cocktail with Tris-Triton X-100 buffer offers a powerful and versatile solution for protein extraction and preservation in biochemical research. This formulation typically consists of a broad-spectrum protease inhibitor cocktail containing AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A, mixed with a buffer composed of Tris-HCl (pH 7.5), NaCl, and Triton X-100. This unique blend provides comprehensive protection against various classes of proteases while maintaining a stable, non-denaturing environment for proteins. The Tris component ensures a consistent pH, while Triton X-100 facilitates the solubilization of both cytosolic and membrane-bound proteins. This combination is particularly effective for cell lysis, protein extraction, Western blotting, immunoprecipitation, and various enzymatic assays. Its ability to preserve protein structure and function makes it an invaluable tool for researchers working with a wide range of proteins, from small cytosolic enzymes to large membrane-associated complexes, ensuring the integrity of samples throughout experimental procedures.
The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor. This cocktail effectively minimizes protein degradation in a wide range of experimental procedures, including cell lysis, protein purification, and enzymatic assays. The carefully balanced concentrations of each component ensure optimal inhibition of proteolytic activity while maintaining compatibility with most downstream applications, making it an invaluable tool for researchers working with sensitive protein samples.
Advantages:
- Protease inhibitor cocktails contain multiple inhibitors targeting different classes of proteases, providing broad-spectrum protection against protein degradation.
- The Tris-Triton X buffer effectively lyses cells and solubilizes proteins, while the protease inhibitors prevent degradation.
- Improves the yield of intact proteins for downstream applications.
- By preventing proteolysis, this combination helps maintain the native structure and function of proteins, which is crucial for accurate analysis in various biochemical and molecular biology experiments.
Application:
- Western Blotting: It efficiently lyses cells and solubilizes proteins, which are then separated by SDS-PAGE and detected using specific antibodies.
- Protein Extraction and Preservation: The primary application of this mixture is in protein extraction and preservation. When cells or tissues are lysed, endogenous proteases are released and can rapidly degrade proteins of interest. The protease inhibitor cocktail prevents this degradation, while the Tris-Triton X buffer facilitates cell lysis and protein solubilization.
- Drug Development: The protease inhibitor cocktail with Tris-Triton X buffer plays a crucial role in drug development processes, particularly in screening extracts for proteolytic activity, studying proteolysis in cellular processes and developing and testing protease inhibitors as potential drugs.
- Cell and Tissue Culture: This mixture is valuable in cell and tissue culture applications, helping to maintain protein integrity during experiments involving cell funtion studies, natural cell regulation research and microbial defense investigations.
Composition:
| Ingredients |
Concentration |
| AEBSF.HCl |
100 uM |
| Aprotinin |
0.8 uM |
| Bestatin |
0.05 mM |
| E-64 |
1 uM |
| EDTA |
5 mM |
| Leupeptin |
0.01 mM |
| Pepstatin A |
1 uM |
| Triton-X |
1% |
| Tris-HCl |
50 mM |
| Sodium Chloride |
150 mM |
Quality Control:
-
Appearance: Clear solution.
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -80 ℃
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Shelf life: 3 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
