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[Please Inquire] FluxMPS™ Glasgow's MEM (GMEM) w/o Threonine and Serine: 1X Liquid

Product#: DCP-GMEM-YTYS1X
$77.00
DCP-GMEM-YTYS1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

MOQ required. Lead time: 4–5 months. Please inquire before ordering.

FluxMPS™ Glasgow's MEM (GMEM) w/o Threonine and Serine: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without Threonine Without Serine

FluxMPS™ DCP-GMEM-YTYS1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Glasgow's MEM (GMEM) without Threonine and Serine formulation engineered for BHK-21 (Baby Hamster Kidney) and related renal cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red | [-] Threonine, [-] Serine.

  • Glasgow's MEM (GMEM) formulated without Threonine and Serine — supports amino acid drop-out studies of one-carbon metabolism and serine biosynthesis in BHK-21 and renal cell models
  • High glucose base: 4500 mg/L (4.5 g/L) D-Glucose, 292 mg/L L-Glutamine, and 2750 mg/L sodium bicarbonate buffering
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off — Microfluidics Suitable for OoC and MPS platforms
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>)
  • 29 defined components across inorganic salts, amino acids, vitamins, glucose and phenol red — every batch released against a full specification matrix with CAS numbers
  • Manufactured under an ISO 13485:2016 quality management system; final QC performed at Diagnocine, Totowa, NJ
  • MOQ required; lead time 4–5 months — contact support@diagnocine.com to inquire before ordering
  • Custom formulation modifications (pH, glucose concentration, salts, nutrient composition) available on request
CAT. NO.
DCP-GMEM-YTYS1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Glasgow's MEM (GMEM) w/o Threonine and Serine: 1X Liquid
  • Media familyGlasgow's MEM (GMEM) without Threonine and Serine
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Threonine, [-] Serine
  • AppearanceRed-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Glasgow's MEM passes mycoplasma-scale organisms, subvisible particulates, and endotoxin fragments that confound sensitive cell culture applications. FluxMPS™ reduces these risks with a four-stage, sub-0.04 µm filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 particulate compliance support safe perfusion in microfluidic chip geometries.

target

Specialized amino acid control

Threonine and serine are both absent, enabling controlled one-carbon metabolism and serine biosynthesis studies. Glasgow's MEM otherwise carries 2× amino acid and vitamin concentrations versus standard MEM, with 4.5 g/L glucose and sodium bicarbonate buffering.

water_drop

Ultrapure-grade water

Type 1 water at 18.2 MΩ·cm supports trace-metal and organic-carbon control for sensitive cell culture applications.

visibility

Low background for imaging

Quadruple-stage filtration reduces the particulate baseline for confocal microscopy and optical biosensor applications on chip.

science

Rich, stable nutrient profile

Glasgow's MEM delivers specialized nutrients for demanding renal and fibroblast-derived cell types, released per lot with full composition verification.

tune

Customization on demand

pH, glucose concentration, salts, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-GMEM-YTYS1X is processed through a four-pass, two-pair filtration train reaching a 0.04 µm final cut-off, well below the 0.22 µm standard used for conventional cell culture media.

0.04
µm final pore size — sub-mycoplasma polishing
4
Filtration passes across two prefilter + final-filter pairs
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-GMEM-YTYS1X Glasgow's MEM (GMEM) w/o Threonine and Serine: 1X Liquid ? Quadruple-stage filtration system, 0.04?μm final polish, Microfluidics Suitable Glasgow's MEM for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-GMEM-YTYS1X
Applications

Specialized cell culture applications

FluxMPS™ DCP-GMEM-YTYS1X — Glasgow's MEM (GMEM) without Threonine and Serine — engineered for BHK-21 (Baby Hamster Kidney) and renal cell models, with 0.04 µm filtration purity for OoC compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered Glasgow's MEM prevents microchannel clogging for complex OoC platforms.

OoCMPSToCLoC
Renal Biology

BHK-21 & Renal Cell Culture

GMEM with 2× amino acid/vitamin concentrations supports BHK-21 kidney cells and renal cell lines for gene expression studies.

BHK-21Renal cellsKidney-on-chip
Virology

Viral Vector & Vaccine Production

BHK-21 cells cultured in Glasgow's MEM support viral vector and vaccine production workflows requiring low-particulate, low-endotoxin media.

BHK-21Viral vectorsVaccine production
Primary Cells

Primary Cell Culture

Sub-mycoplasma-grade filtration and a < 0.05 EU/mL endotoxin specification support sensitive primary cell models.

Primary fibroblastsPrimary epithelialPrimary neurons
Metabolomics

Metabolic Flux Analysis

Defined amino-acid drop-out formulation base for ¹³C isotope tracing and metabolic flux studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingMetabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Quadruple-stage filtration reduces particulate baseline for confocal microscopy and biosensor OoC applications.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Threonine, [-] Serine
Appearance Red-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 29 components across 4 category groups (Inorganic Salts, Amino Acids, Vitamins, Others)
Available pack sizes 500 mL, 1000 mL
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 7.2% CO2, calculated from 2750 mg/L NaHCO3 to maintain pH 7.4 (verify empirically per incubator/vessel)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glasgow's MEM (GMEM) without Threonine and Serine: 29 ingredients verified per lot with CAS numbers. Threonine and serine are both absent. GMEM otherwise contains 2× amino acid and vitamin concentrations vs. standard MEM, with 4.5 g/L glucose and sodium bicarbonate buffer. Note: MOQ required, lead time 4–5 months — contact support@diagnocine.com.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2750.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 42.000
L-Cystine dihydrochloride 30925-07-6 31.290
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride 1007-42-7 21.000
L-Isoleucine 73-32-5 52.400
L-Leucine 61-90-5 52.400
L-Lysine hydrochloride 657-27-2 73.100
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 33.000
L-Tryptophan 73-22-3 8.000
L-Tyrosine disodium salt dihydrate 69847-15-0 52.000
L-Valine 72-18-4 46.800
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxal hydrochloride 65-22-5 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
i-Inositol 87-89-8 3.600
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.000
Custom formulation: Contact support@diagnocine.com for DCP-GMEM-YTYS1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water supports trace-metal and organic-carbon control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch (assay sensitivity 0.005 EU/mL).

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); light obscuration.

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-GMEM-YTYS1X compares

FluxMPS™ DCP-GMEM-YTYS1X vs. conventional 0.22 µm–filtered Glasgow's MEM and standard DMEM/RPMI formulations.

Parameter DCP-GMEM-YTYS1X (FluxMPS™) Conventional Glasgow's MEM
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
GMEM without Threonine and Serine — amino acid drop-out for BHK-21 and renal cell studies check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (two prefilter + final-filter pairs) 1 1
Mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Compatible cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEM-YTYS1X — Glasgow's MEM (GMEM) without Threonine and Serine.

Yes. DCP-GMEM-YTYS1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels for MPS, OoC, and LoC platforms. The Glasgow's MEM (GMEM) without Threonine and Serine formulation carries a < 0.05 EU/mL endotoxin release specification for demanding cell culture applications on chip.
FluxMPS™ uses four sequential filters run as two dedicated prefilter + final-filter pairs — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off with 0.1 µm mycoplasma-retentive filtration at each prefilter stage, versus a single 0.22 µm pass for conventional media.
Threonine and serine are removed to create selective amino acid drop-out conditions for studying one-carbon metabolism (serine is a major donor for folate-mediated one-carbon transfer reactions), serine biosynthesis pathway activity (PHGDH, PSAT1, PSPH), threonine catabolism, and metabolic flux studies in BHK-21 and renal cell lines where these specific amino acid depletions are experimental variables. This formulation also supports selective pressure in auxotroph screening and nutritional studies of cell-type specific amino acid dependencies. Contact support@diagnocine.com for custom re-addition of either amino acid.
This formulation contains 2750 mg/L sodium bicarbonate as the primary buffer, which requires an elevated CO₂ atmosphere to maintain pH 7.4 — approximately 7.2% CO₂, calculated from the stated bicarbonate concentration by the Henderson-Hasselbalch relationship. Verify empirically for your specific incubator and vessel geometry.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. When supplementing with serum or other protein-containing components, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — do not use a 0.04 µm filter for serum-containing additions, as it will retain immunoglobulins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Glasgow's MEM (GMEM) without Threonine and Serine in cell culture and OoC applications.

  1. McPherson I, Stoker M. Polyoma transformation of hamster cell clones — an investigation of genetic factors affecting cell competence. Virology. 1962;16:147–151. doi:10.1016/0042-6822(62)90290-8
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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