Does this medium require a CO2 incubator?

Yes. The formulation is buffered with sodium bicarbonate (2200 mg/L) and contains no HEPES, so a CO2 incubator is recommended to maintain pH 7.4 in open culture.

What endotoxin level should I expect, and how is it verified?

The medium is released to a less than 0.05 EU/mL specification, verified by the USP less than 85> bacterial endotoxin test (BET) and reported on the per-lot Certificate of Analysis.

FluxMPS™ Minimum Essential Medium Alpha (MEMα) w/ Nucleosides: 1X Liquid

Q: Is DCP-MEA1X suitable for organ-on-a-chip (OoC) applications?

Yes. Its 0.04 micronm final filtration delivers ultra-low particulate fluid that flows freely through microchannels and chip geometries, making it well suited for OoC, MPS and microfluidic perfusion devices.

Q: What makes the filtration process superior to standard 0.22 micronm filtered media?

The medium passes through four sequential stages - 0.1 micronm twice then 0.04 micronm twice. The 0.04 micronm stages remove fine particulates and retain mycoplasma (smallest types ~0.2 micronm), yielding roughly 5× lower particulate counts than single-pass 0.22 micronm media.

Q: Why is the glucose kept low at 1.0 g/L, and how do I adjust it for my cell type?

MEMα uses a 1.0 g/L low-glucose base that suits primary cells, stem cells and lines sensitive to high glucose, where elevated glucose can drive ROS and altered signaling. Higher glucose or other modifications can be custom-formulated on request.

Q: Can DCP-MEA1X be supplemented with serum, growth factors, or other components?

Yes. It is typically supplemented with FBS for optimal growth and accepts growth factors and other additives. Custom pre-supplemented formulations are available on request.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium Alpha (MEMα) w/ Nucleosides: 1X Liquid

An MPS–grade, nucleoside–enriched MEMα liquid medium polished through a Quadruple–stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for ultra–low particulate and mycoplasma–safe performance. Built for microfluidic channels and organ–on–a–chip devices, it is approximately 5× cleaner than conventional 0.22 µm–filtered media by particulate count, with ribonucleosides and deoxyribonucleosides supplying enhanced metabolic support. Formulation: [+] 1.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate [+] Sodium Bicarbonate, [+] Phenol Red, [+] Ribonucleosides, [+] Deoxyribonucleosides

Nano–filtration to 0.04 µm for ultra–low subvisible particulate and unobstructed microchannel flow.
Quadruple–stage filtration: 0.1 µm twice + 0.04 µm twice for redundant sterility and mycoplasma retention.
Endotoxin specification < 0.05 EU/mL (USP <85> BET).
Enriched MEMα base with non–essential amino acids, vitamins, ribonucleosides and deoxyribonucleosides.
1.0 g/L low–glucose formulation with L–glutamine and sodium pyruvate; no HEPES.
Compounded with Ultrapure Type 1 water (18.2 MΩ·cm).
Final aseptic fill in a validated ISO Class 5 (Class 100) workstation.
pH, glucose, salts, HEPES and nutrient composition customizable on request.

DCP-MEA1XUNSPSC 41122100Cell Culture Media

FluxMPS™ MEMα w/ Nucleosides — 1X Liquid

Glucose1000 mg/L
L–Glutamine292 mg/L
Sodium Pyruvate110 mg/L
pH (USP <791>)7.4
OsmolalityPer–lot CoA
Endotoxin< 0.05 EU/mL
Filtration0.1µm×2 + 0.04µm×2
Storage2–8°C
Shelf Life12 months
ShippingCold pack

ISO 13485:2016USP <85> <785> <788>RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media leaves subvisible particulates and can permit mycoplasma breakthrough — failure modes that clog microfluidic channels, accumulate in chip geometries, and corrupt optical and biosensor signals.^[1,2] FluxMPS™ MEMα w/ Nucleosides re–engineers this enriched formulation for microphysiological systems, pairing a nucleoside–supplemented nutrient base with a Quadruple–stage filtration architecture.

filter_alt

Microchannel–safe purity

A 0.04 µm final filter drives subvisible particulate counts well below USP <788> limits, keeping micro–geometries and perfusion lines clear.

target

Total metabolic control

A defined 1.0 g/L low–glucose carbon source with ribonucleosides and deoxyribonucleosides supports demanding proliferation and Warburg–type metabolic studies.

water_drop

Ultrapure–grade water

Compounded with Ultrapure Type 1 water (18.2 MΩ·cm) and released against USP <85> endotoxin criteria.

visibility

Low background for imaging

Ultra–clean, low–particulate fluid minimizes scatter and autofluorescence for confocal microscopy and on–chip biosensors.

science

Rich, stable nutrient profile

Enriched MEMα base with non–essential amino acids, vitamins and nucleosides, produced in micro–batches for lot–to–lot consistency.

tune

Customization on demand

pH, glucose concentration, salts, HEPES and nutrient composition are available on request — contact support@diagnocine.com.

Purity Architecture

Quadruple–stage filtration system

A serial, four–stage sequential filtration process reaching 0.04 µm. To our knowledge this is the only ready–to–use nucleoside–enriched MEMα offered at this purity level for microfluidic and organ–on–a–chip work.

1

0.1 µmPre–filtration I

Removes large particulates and aggregates, guarding downstream filter life and chip geometries.
2

0.04 µmPre–filtration II

Removes fine particulates, bacteria and mycoplasma; typical mycoplasma (0.1–0.3 µm) are retained at this stage — a step absent in standard 0.22 µm filtration.
3

0.1 µmSterile–filtration I

Second–pass removal of residual contaminants and bioburden, providing redundancy with no breakthrough from stage 1.
4

0.04 µmSterile–filtration II — Final Polish

Ultimate polish for ultra–pure, particle–free, mycoplasma–free media; final fill performed in a validated ISO Class 5 (Class 100) laminar–flow workstation under aseptic conditions.

Performance vs. conventional media

By driving the final filtration to 0.04 µm, FluxMPS™ MEMα w/ Nucleosides reaches approximately 5× lower particulate counts than conventional 0.22 µm–filtered media, enabling unobstructed microfluidic flow and clean imaging backgrounds.

5×

cleaner by particulate count vs 0.22 µm media

0.04

µm final filtration pore size

Sterility & mycoplasma: Filtered 0.1 µm twice and 0.04 µm twice in a sterile environment; no bacterial or fungal growth after 14 days of incubation per USP specification. Because the smallest mycoplasma are about 0.2 µm, the 0.04 µm stages provide USP <63>–equivalent mycoplasma assurance.

FluxMPS™ Minimum Essential Medium Alpha (MEMα) with Nucleosides DCP-MEA1X, MPS-grade cell culture media manufactured with the Quadruple-stage filtration system (0.1 μm ×2 + 0.04 μm ×2) for organ-on-a-chip and microfluidic applications | Diagnocine — **Figure 1.** Serial four–stage architecture — 0.1 µm pre–filtration twice followed by 0.04 µm sterile–filtration twice — delivering ultra–low particulate, mycoplasma–safe MEMα for microfluidic and organ–on–a–chip use.
© Diagnocine® — DCP-MEA1X

Applications

Built for microphysiological systems

The nucleoside–enriched, low–glucose MEMα profile supports adherent, suspension, primary and transfected cell lines that benefit from a more complete nutritional base inside chips, perfusion devices and imaging platforms.^[3,4]

Automated Bioreactors & Robotics

Next–Generation System Uptime

For automated bioreactors and robotic perfusion platforms, an optional 0.01 µm (10 nm) ultra–filtered variant pushes particulate exclusion further to protect precision valves, sensors and long perfusion runs.

Total Particulate Exclusion: 10 nm filtration removes the finest residual particulates ahead of microvalves and flow cells.
Valve & Sensor Protection: Minimizes fouling of automated fluidics, dispensers and in–line optical sensors.
Extended Perfusion Stability: Cleaner feed supports longer, uninterrupted perfusion and reduced maintenance downtime.

Inquiry Required: The 0.01 µm (10 nm) grade is made to order — contact support@diagnocine.com to request it.

Microfluidics