FluxMPS™ Minimum Essential Medium Alpha (MEMα) w/ Nucleosides: 1X Liquid
FluxMPS™ Minimum Essential Medium Alpha (MEMα) w/ Nucleosides: 1X Liquid
A Microfluidics Suitable, nucleoside–enriched MEMα liquid medium polished through a Quadruple–stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for ultra–low particulate performance. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Nano–filtration to 0.04 µm for ultra–low subvisible particulate and unobstructed microchannel flow.
- Quadruple–stage filtration: 0.1 µm twice + 0.04 µm twice, run as two dedicated prefilter/final–filter pairs for redundant sterility assurance.
- Endotoxin release specification < 0.05 EU/mL, verified by LAL assay per USP <85> (Bacterial Endotoxins Test).
- Enriched MEMα base with non–essential amino acids, vitamins, ribonucleosides and deoxyribonucleosides.
- 1.0 g/L low–glucose formulation with L–glutamine (292 mg/L) and sodium pyruvate (110 mg/L); formulated without HEPES.
- Compounded with Ultrapure Type 1 water (18.2 MΩ·cm).
- Final aseptic fill in a validated ISO Class 5 (Class 100) laminar–flow workstation.
- pH, glucose, salts, HEPES and nucleoside composition are customizable on request.
- Glucose1000 mg/L
- L–Glutamine292 mg/L
- Sodium Pyruvate110 mg/L
- pH (USP <791>)7.4
- OsmolalityReported per lot on CoA
- Endotoxin< 0.05 EU/mL
- Filtration0.1µm×2 + 0.04µm×2
- Storage2–8°C, protect from light
- Shelf Life12 months from manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered media leaves subvisible particulates behind and provides no dedicated mycoplasma–size retention — failure modes that clog microfluidic channels, accumulate in chip geometries, and corrupt optical and biosensor signals.[1,2] FluxMPS™ MEMα w/ Nucleosides re–engineers this enriched formulation for microphysiological systems, pairing a nucleoside–supplemented nutrient base with a Quadruple–stage filtration architecture.
Microchannel–safe purity
A 0.04 µm final filter drives subvisible particulate counts well below USP <788> limits, keeping micro–geometries and perfusion lines clear.
Total metabolic control
A defined 1.0 g/L low–glucose carbon source with ribonucleosides and deoxyribonucleosides supports demanding proliferation and Warburg–type metabolic studies.
Ultrapure–grade water
Compounded with Ultrapure Type 1 water (18.2 MΩ·cm) for low ionic and organic (TOC) background. Endotoxin is independently controlled to a per–batch release specification.
Low background for imaging
Ultra–clean, low–particulate fluid keeps the particulate baseline low for confocal microscopy and on–chip biosensors.
Rich, stable nutrient profile
Enriched MEMα base with non–essential amino acids, vitamins and nucleosides, produced in micro–batches for lot–to–lot consistency.
Customization on demand
pH, glucose concentration, salts, HEPES and nucleoside composition are available on request — contact support@diagnocine.com.
Quadruple–stage filtration system
A serial, four–stage sequential filtration process reaching a 0.04 µm final pore size, purpose–built for microfluidic channels and organ–on–a–chip work requiring a nucleoside–enriched MEMα base.
-
1
0.1 µmPrefiltration I
Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge downstream.
-
2
0.04 µmFinal filtration I
First 0.04 µm pass; retains sub–micron particulates and mycoplasma–sized organisms (0.2–0.3 µm) that pass through standard 0.22 µm filtration.
-
3
0.1 µmPrefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge and providing redundancy in the train.
-
4
0.04 µmFinal filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in a validated ISO Class 5 (Class 100) laminar–flow workstation.
Performance vs. conventional media
© Diagnocine® — DCP-MEA1X
Built for microphysiological systems
The nucleoside–enriched, low–glucose MEMα profile supports adherent, suspension, primary and transfected cell lines that benefit from a more complete nutritional base inside chips, perfusion devices and imaging platforms.[3,4]
Automated Bioreactors & Robotics
For automated bioreactors and robotic perfusion platforms, an optional 0.01 µm (10 nm) ultra–filtered MPS Grade variant of this formulation pushes particulate exclusion further to protect precision valves, sensors and long perfusion runs.
- Total Particulate Exclusion: 10 nm filtration removes the finest residual particulates ahead of microvalves and flow cells.
- Valve & Sensor Protection: Minimizes fouling of automated fluidics, dispensers and in–line optical sensors.
- Extended Perfusion Stability: Cleaner feed supports longer, uninterrupted perfusion and reduced maintenance downtime.
Inquiry Required: The 0.01 µm (10 nm) MPS Grade variant is made to order — contact support@diagnocine.com to request it.
Micro Physiological System (MPS) & Chip
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application.
Warburg Effect & Metabolic Research
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application.
iPSC–Derived Models
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application.
Endothelial & Primary Cells
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application.
Metabolic Flux Analysis
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application. Not compatible with Agilent Seahorse XF assays, which require bicarbonate–free, phenol red–free medium.
Microscopy & Optical Sensing
Optimized fluid purity and a nucleoside–rich nutrient base for reproducible performance in this application.
Specifications at a glance
Physical, chemical, sterility, storage and traceability parameters for FluxMPS™ MEMα w/ Nucleosides (DCP-MEA1X).
| Parameter | Specification |
|---|---|
| Formulation | MEMα base with L–Glutamine, Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, Glucose (1.0 g/L) and Sodium Pyruvate; HEPES not added; supplemented with ribonucleosides and deoxyribonucleosides |
| Appearance | Red–colored, clear solution |
| pH (USP <791>) | 7.4 USP <791> |
| Osmolality (USP <785>) | Reported per lot on CoA USP <785> |
| Glucose | 1000 mg/L (1.0 g/L) |
| L–Glutamine | 292 mg/L |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | Present (11.000 mg/L) |
| Parameter | Specification |
|---|---|
| Endotoxin (USP <85> BET) | < 0.05 EU/mL USP <85> |
| Sterility (USP <71>) | No growth, 14–day incubation USP <71> |
| Mycoplasma | 0.1 µm mycoplasma–retentive filtration (not tested per lot) |
| Particulate ≥10 µm | Compliant USP <788> Method 1 |
| Particulate ≥25 µm | Compliant USP <788> Method 1 |
| Water purity | Ultrapure Type 1 (18.2 MΩ·cm) |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) ISO Class 5 |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from bright light |
| Freeze–thaw | Avoid; store refrigerated |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | Sodium bicarbonate (2200 mg/L) buffered; approximately 5.8% CO2 recommended to maintain pH 7.4 |
| Parameter | Specification |
|---|---|
| Raw material grade | High–purity cell–culture grade |
| Traceability | Full lot traceability with CoA |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro–batch, Quadruple–stage filtered |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Enriched MEMα base supplemented with ribonucleosides and deoxyribonucleosides. Values are released on a per–lot basis; all 49 components are listed below with CAS numbers, organized across three tabs: Inorganic Salts, Amino Acids, and Vitamins & Others.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 50.000 |
| L-Alanine | 56-41-7 | 25.000 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 50.000 |
| L-Aspartic acid | 56-84-8 | 30.000 |
| L-Cystine dihydrochloride | 30189-89-0 | 31.300 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 100.000 |
| L-Glutamic acid | 56-86-0 | 75.000 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 40.000 |
| L-Serine | 56-45-1 | 25.000 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 122666-87-9 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Ascorbic Acid | 50-81-7 | 50.000 |
| Choline chloride | 67-48-1 | 1.000 |
| D-Biotin | 58-85-5 | 0.100 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 1.360 |
| OTHERS | ||
| 2' Deoxyadenosine | 16373-93-6 | 10.000 |
| 2' Deoxycytidine hydrochloride | 3992-42-5 | 11.000 |
| 2' Deoxyguanosine | 961-07-9 | 10.000 |
| Adenosine | 58-61-7 | 10.000 |
| Cytidine | 65-46-3 | 10.000 |
| D-Glucose | 50-99-7 | 1000.000 |
| Guanosine | 118-00-3 | 10.000 |
| i-Inositol | 87-89-8 | 2.000 |
| Lipoic acid | 1077-28-7 | 0.200 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| Thymidine | 50-89-5 | 10.000 |
| Uridine | 58-96-8 | 10.000 |
Manufacturing & compliance
Produced under an ISO 13485:2016 quality management system, with final packaging, QA and testing at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Manufactured under a certified medical–device quality management system, 21 CFR Part 820 (QMSR) aligned.
Ultrapure Type 1 Water
Compounded with 18.2 MΩ·cm water to minimize ionic and organic (TOC) background.
ISO Class 5 Fill & Finish
Final aseptic fill in a validated ISO Class 5 (Class 100) laminar–flow workstation.
Micro–Batch Precision
Small–batch production for tight lot–to–lot consistency and traceability.
Endotoxin — USP <85> BET
Released to a < 0.05 EU/mL specification by the LAL bacterial endotoxin test.
Particulate — USP <788> Method 1
Subvisible particulate compliant for ≥10 µm and ≥25 µm thresholds by light obscuration.
Osmolality — USP <785>
Measured and reported on the per–lot Certificate of Analysis.
Documentation / CoA
Each lot ships with a Certificate of Analysis covering identity, pH, sterility and endotoxin.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-MEA1X compares
FluxMPS™ MEMα w/ Nucleosides versus conventional and standard 0.22 µm–filtered MEMα media.
| Parameter | DCP-MEA1X (FluxMPS™) | Conventional MEMα (0.22 µm filtered) | Standard MEMα (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Nucleoside enrichment | Ribo + deoxyribonucleosides | Variable | Often absent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (0.1×2 + 0.04×2) | 1–2 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle | Partial | cancel |
| Water quality | Ultrapure Type 1 | Type 1/2 | Type 2 |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | Limited | cancel |
| Custom formulation | check_circle | Limited | cancel |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ MEMα w/ Nucleosides (DCP-MEA1X).
Supporting literature
Selected peer–reviewed literature on microphysiological systems, microfluidic culture and media purity relevant to this formulation.
- Bhatia SN, Ingber DE. Microfluidic organs–on–chips. Nature Biotechnology. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Huh D, et al. Reconstituting organ–level lung functions on a chip. Science. 2010;328(5986):1662–1668. doi:10.1126/science.1188302
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
- Stanton CR, et al. Engineering cell culture media for microphysiological systems. Lab on a Chip. 2020;20(3):446–467. doi:10.1039/C9LC00925F
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324(5930):1029–1033. doi:10.1126/science.1160809
- Nikolaev M, et al. Homeostatic mini–intestines through scaffold–guided organoid morphogenesis. Nature. 2020;585:574–578. doi:10.1038/s41586-020-2724-8
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Ronaldson–Bouchard K, Vunjak–Novakovic G. Organs–on–a–chip: a fast track for engineered human tissues. Cell Stem Cell. 2018;22(3):310–324. doi:10.1016/j.stem.2018.02.011
- Zhang B, et al. Advances in organ–on–a–chip engineering. Nature Reviews Materials. 2018;3:257–278. doi:10.1038/s41578-018-0034-7
- Yu F, et al. A perfusion incubator liver chip for 3D cell culture. Scientific Reports. 2017;7:14528. doi:10.1038/s41598-017-13848-5