Pfu Super DNA Polymerase(+dNTPs)

Cat.No:  FNK-02-022

Size: 200 U

Storage: -20℃

Concentrarion: 2.5 U/µl

Purity: Greater than 95% of protein determined by SDS-PAGE (CBB staining)(Fig.1) The absence of endonucleases and exonucleases was confirmed.

PCR Test: Good amplification result was obtained in PCR reaction using ?DNA as a template (Fig.2).
 

Product Description

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.

• Pfu DNA polymerase is thermostable and has low error rates.
• It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
• Pfu DNA polymerase-generated PCR fragments are blunt-ended

Definition of Activity

One unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.

Components

Pfu Super DNA polymerase (2.5U/µl): 50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.1% Tween20, 0.1% Igepal CA-630 (02-Spd 80 µl)

10 x Standard Buffer (Pfu): 200 mM Tris-HCl (pH 8.8), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Triton X-100, 1 mg/ml BSA (02-Psd 1.0 ml)

2.5 mM (each) dNTPs: (02-Dnt 640µl)

Application

Cloning long genes after PCR amplification High-fidelity DNA amplification in genreal  Site-directed mutagenesis

 

Please Note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. NOT FOR MILITARY USE.
 

Data Image

02-022 Pfu Super DNA Polymerase with Standard Buffer (+dNTPs)

Fig.1 SDS-PAGE OF PFU DNA polymerase



Fig.2 Amplifaction of λDNA

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