Ordering Information

Available pack sizes of KOD One™ PCR Master Mix.

Description

KOD One^TM PCR master Mix and KOD One^TM PCR Master Mix -Blue- are 2 x PCR master mixes based on genetically modified KOD DNA polymerase (UKOD). KOD One^TM series enables fast PCR, which has an extension time of 5 sec/kb by applying UKOD and a new Elongation Accelerator. In addition, these master mixes provide greater efficiency and elongation capabilities than conventional PCR enzymes. In particular, these show greater amplification success from crude specimens. Furthermore, these master mixes can be applied to amplify from templates containing uracils (dU) or using primers containing inosines (dI) and uracils (dU).

KOD One^TM series contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3’→5’ exonuclease activities, thus allowing for Hot Start PCR. These master mixes generate blunt-end PCR products because of 3’→5’ exonuclease (proof-reading) activity of KOD DNA polymerase.

Features

Applications

Source

Components

KOD One^TM series include the following components for 200 reactions, 50 µl total reaction volume.

Application Data

Example 1. Fast PCR

Various targets were amplified with KOD One^TM PCR Master Mix and KOD One^TM PCR Master Mix -Blue- using the ultra fast cycling conditions. KOD One^TM series successfully amplified all targets.

Example 2. Amplification from cDNA

Inhibitory effect of RNA in cDNA was compared using various PCR enzymes. KOD One^TM Master Mix was not susceptible to RNA inhibition, and it was able to amplify targets under high concentrations of cDNA.

Example 3. PCR error ratio

The error ratio of various PCR enzymes were compared by determining the sequences of the amplicons from human β-globin gene. The amplicons were cloned into the vector using TArget clone^TM -Plus- (Code No. TAK-201) and the sequences were determined.

KOD One^TM PCR Master Mix and KOD One^TM PCR Master Mix -Blue- showed excellent fidelity and the mutation frequency were approximately 80 times lower than Taq DNA polymerase.

Example 4. Amplification using degenerate primers, containing inosine (I)

The 2.8 kb fragments were amplified using degenerate primers containing inosine (I). KOD One^TM PCR Master Mix was able to amplified, whereas conventional high-fidelity PCR enzymes cannot.

Example 5. Amplification from crude samples

Amplification from whole blood and that from mouse lysate were compared. KOD One^TM PCR Master Mix amplified the targets efficiently.

Compared to Other Products in PCR Master Mix

(Third-Party experimental data shows) — KOD One is superior to Q5

(1) Greater than ten times better than ThermoFisher DreamTaq for High GC Content PCR

Do you have a high GC content or touch gene to amplify? KOD One High Fidelity PCR master mix is ten times better than Dream Taq (Thermo Fisher) for High GC Content template (amplification of PIPPA gene region)

No band due to a really tough GC region? KOD One (product #KMM-101 or 201 series) is 10X superior to Dream Taq, generating that band for you from all High GC content regions.

(2) Greater than ten times better than Invitrogen or ThermoFisher Platinum Taq (High Fidelity Fast PCR)

Wish to generate solid PCR bands? KOD One Master Mix (product #KMM-101 or 201 series) is more than ten times better than Invitrogen | Thermo Fisher Platinum Taq (amplification of XTL1127 region).

KOD One is an extremely effective enzyme that will amplify tough genes and DNA templates. Abundant PCR amplification allows you to load a small amount of PCR product and save PCR samples for later use.

(3) Three times better than NEB OneTaq (Expression Analysis by PCR)

Want something better and stronger than NEB One Taq? KOD One PCR master mix (product #KMM-101 or 201 series) is at least three times better than One Taq for amplifying tough templates.

Researchers confirm that 3X superior amplification for A5 and B6 genes and make solid PCR bands. Excellent enzyme to amplify low abundance genes and tough genomic regions.

(4) 30% to 46% lower price than NEB Q5

Want to save budget? KOD One PCR master mix (product #KMM-101 or 201 series) will save you 50% compared to NEB Q5. Buy one unit will save near 30%. Buy 5 set bundles and save more than 35% compared to major brands in the market. Buy 10 set bundle and the KOD One mix is near half price compared to other name brands. Price comparison data showed KOD One master mix is the top choice for high fidelity PCR master mix and the biggest bang for the buck in the market.

(5) Five times better GC-Rich Region gDNA PCR result than Takara GXL System or Clontech GXL System

Want to maximize PCR amplification in the tough template region? KOD One PCR master mix (product #KMM-101 or 201 series) amplifies five times better than Takara GXL System. KOD One mix will generate bands from tough templates with 3X superior amplification than Clontech GXL. KOD One mix is excellent for an implication of long amplicon (10Kb+), High GC rich regions, low expressed genes and secondary structures templates.

(6) More than ten times better amplification from crude samples than other brands

High efficiency: excellent amplification from crude samples

Cannot generate PCR band from a crude sample and unpurified templates? KOD will do it for you. KOD One PCR master mix (product #KMM-101 or 201 series) is an extremely efficient enzyme that can work on crude and dirty samples. KOD One mix is more than ten times better than other major brands. 10X superiority compared to other major brands and with some optimization, generating PCR band from crude samples can be done without much preparation.

(7) 11 kb long range for all organisms with high fidelity (10Kb+ gDNA Long Range PCR)

25 ul Rx vol., 25-35 cycles, gDNA Long range PCR

Need a long rang PCR? 10kb or more needed with minimum errors? KOD One PCR master mix (product #KMM-101 or 201 series) can provide greater than 10Kb and will satisfy all long-range PCR experiments. Long PCR works from bacteria to humans with difficult regions on DNA templates. KOD One is highly efficient in generating large-size PCR bands with minimal errors (1 error in 10 million base pairs).

(8) 80 times higher fidelity than Taq DNA polymerase and Top Class in the market

Want top high fidelity PCR enzyme and robust amplification of high GC content, long PCR, crude samples, minimum errors. Look no further because the KOD One PCR master mix (product #KMM-101 or 201 series) works above and beyond to give you PCR band at the lowest price per PCR reaction. It is the top-class high fidelity PCR enzyme master mix in the market. KOD One is 80 times higher fidelity than traditional Taq DNA polymerase. It is the most robust PCR enzyme engineered to give you the PCR band you need.

(9) Ten times faster than traditional PCR enzymes (1kb per one-second extension)

Looking for a lighting fast PCR enzyme? KOD One PCR master mix (product #KMM-101 or 201 series) will amplify ten times faster than the traditional PCR enzyme mix. 1kb elongation per 1 second extension time gives you 10X times faster PCR time to shorten your bench time and quickly see the result. Shorten your PCR time for long-range amplification with minimum error. Superior and fast enzyme for 10kb and more length of amplicons from all sources of samples and crude sample preparations.

(10) More than ten times better amplification with RNase inhibitors.

Need robust PCR enzyme with RNase inhibitors? KOD One PCR master mix (product #KMM-101 or 201 series) is more than ten times better than other major brands at amplifying PCR bands with RNase inhibitors in the reaction. Samples with RNase inhibitors are no problem with the KOD enzyme mix. No band or weak band becomes a solid and bright PCR band on your gel. Minimum errors, high fidelity, large PCR bands and lightning-fast amplification for all sources of samples, including crude samples. The next-generation high fidelity PCR enzyme is engineered to amplify PCR bands from GC-rich regions and tough templates.