Peroxidase conjugated anti-Cry j 2 pAb

Product#: HBL-Ab-2-000P
$280.00
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Peroxidase conjugated anti-Cry j 2 pAb


General information 

Cat. No. HBL-Ab-2-000P
Quantity 25 µg, 100 µl/vial (Frozen Liquid)
Specificity This antibody was obtained from Cry j 2 antigen (Japanese cedar pollen allergen) immunized Rabbit
    serum (Japanese White strain). This antibody cross-reacts against Cry j 2 antigen less than 10%.
Preparation & form Rabbit IgG was purified from the anti-Cry j 2 rabbit serum by ProteinG Sepharose column chromatography.
    Then IgG was conjugated with horseradish peroxidase by periodide method. Peroxidase conjugated IgG
    was prepared in the concentration of 250μg/ml with phosphate-buffered saline containing 1 % bovine
    serum albumin fractionV
Format Protein A / G Affinity Purified
Crossing Cryptomeria japonica
Immune animals Rabbit 
Label Unlabeled
Class :IgG
Application   This antibody is suitable for enzyme-linked immunosorbent assay. Dilute 1:1000 as a detect antibody.
Storage Store below -20°C. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots.
 

Description

Pollen, purified antigens (Cry j 1, Cry j 2) and antibodies (anti-Cry j 1 antibody, anti-Cry j 2 antibody) of Japanese Sugi ( Cryptomeria japonica ). It is used in many treatises and boasts a high track record. It is useful for studying Japanese cedar pollen allergy.

It is an antibody produced using Cry j 2 of Japanese Sugi ( Cryptomeria japonica ) as an immunogen.

* This product is for research use. It cannot be used for anything other than research.


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What is Japanese cedar pollen?

Two main types of cedar pollen allergens are known as the causative agents of Japanese cedar pollinosis. One is Cry j 1, a basic protein with a molecular weight of about 40 kDa. Cry j 1 is localized in ubiquitous bodies (particle size of about 0.7 μm) attached to the surface of Japanese cedar pollen with a particle size of about 30 μm. The other is Cry j 2, a basic protein with a molecular weight of about 37 kDa. Cry j 2 is thought to be localized in the starch granules inside cedar pollen and in the pollen intima.



References

  1. Sawatani, M et al. : Immunological and physiological properties of Cry j II, the second major allergen of Japanese cedar pollen. [Article in Japanese] Arerugi, 42(6):738-747, 1993
  2. Sawatani, M et al.: Enzyme-linked immunosorbent assay for the quantification of Cry j I and Cry j II. [Article in Japanese] Arerugi, 43(3):467-473, 1993
  3. Wang Seiyaku, et al., Aerosol Research, 23 , 120-126 (2008).
  4. Hiroshi Yasue, "Ayumi of Medicine", 200 , 358-362 (2002).
 
 

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