UltraClean PVSA-Supplemented TBS Buffer, 1X (Tris Buffered Saline), RNase-Safe
Cat No.:
DCP-TBSPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe PBS buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-Supplemented TBS Buffer, 1X is a ready-to-use, high-purity Tris-buffered saline formulated to be RNase-safe for demanding molecular biology, cell biology, and immunology workflows. It is supplemented with polyvinylsulfonic acid (PVSA), a potent, polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, washing, and storage. The buffer has physiological ionic strength and near-neutral pH, making it suitable for use with cells, tissues, proteins, and RNA-containing preparations in sensitive downstream applications.
Applications:
- RNA Handling and Storage: Washing, resuspension, and temporary storage of RNA-containing samples, cells, or tissues where RNase control is critical.
- RNA-Compatible Immunostaining: Wash buffer for IHC/ICC and other antibody-based assays performed on RNA-sensitive samples or combined RNA/protein workflows.
- Western Blot and ELISA: Wash and dilution buffer for antibodies and samples when RNA integrity must be preserved alongside protein detection.
- RT-qPCR and RNA Capture Workflows: Dilution of RNA samples, probes, and antibody-RNA conjugates, and washing of solid supports in workflows requiring strict nuclease control in a phosphate-free buffer.
- General Molecular Biology: Any procedure requiring isotonic TBS with enhanced RNA protection, including gentle cell handling and wash steps where phosphate is not desired.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a wide range of RNases, protecting RNA without relying solely on protein-based inhibitors.
- High Purity: Manufactured from molecular biology-grade components and subjected to multiple fine-pore filtration steps to minimize particulates, microbes, and adventitious nucleases.
- Physiological Ionic Strength and pH: Formulated to maintain osmolarity and pH compatible with mammalian cells and tissues, reducing cellular stress during handling.
- Phosphate-Free: Tris-based saline avoids phosphate, which can interfere with certain enzymes, antibodies, and metal-dependent interactions.
- Ready-to-Use 1X Solution: No dilution or mixing required, minimizing preparation time and contamination risk.
Composition:
| Ingredients |
Concentration |
| Tris Base |
20 mM |
| Sodium Chloride |
150 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
