SigMax 1Ab-PBS
FluxMPS™ SigMax 1Ab-PBS
SigMax 1Ab-PBS is an MPS-grade, PBS-based antibody dilution buffer engineered to enhance antibody-antigen binding while suppressing background noise across immunoassay workflows. Buffered to pH 7.4 with a proprietary PEG 6000, PVP-40, and BSA blend, it is dual-stage filtered through 0.1 µm and 0.04 µm membranes for microchannel-safe, low-particulate performance.
- Dual-stage filtration: 0.1 µm membrane once and 0.04 µm membrane once for ultra-clean, low-particulate liquid
- Buffered to pH 7.4 for consistent antibody-antigen interaction chemistry
- Proprietary PEG 6000, PVP-40, and BSA blend enhances primary antibody binding efficiency by up to 10-fold
- Reduces non-specific binding for cleaner, higher signal-to-noise results
- Manufactured with ultrapure Type 1 water (18.2 MΩ·cm)
- Produced under ISO 13485-certified, CE-approved manufacturing
- Compatible with Western blotting, ELISA, IHC, ICC, flow cytometry, and cell/tissue washing
- pH, concentration, and additive customization available on request — contact support@diagnocine.com
- pH7.4
- AppearanceClear, Colorless Liquid
- SterilityNon-Sterile
- Filtration0.1 µm x1 + 0.04 µm x1
- FormulationPBS + PEG 6000 + PVP-40 + BSA
- Storage4°C
- Shelf Life1 Year
- Size100 mL
- ManufacturingISO 13485 / CE-Approved
- Intended UseRUO
Engineered where standard antibody diluents fall short
Conventional single-pass filtered PBS diluents can carry residual particulates, inconsistent pH, and insufficient background suppression — limiting detection sensitivity in demanding immunoassay formats. SigMax 1Ab-PBS addresses each failure mode directly.
Microchannel-safe purity
Dual-stage filtration finishes at a 0.04 µm membrane pass, reducing particulate carryover in downstream microfluidic and imaging workflows.
Precise, stable pH
Buffered to pH 7.4, preserving native antibody and antigen conformations to maximize epitope recognition.
Ultrapure-grade water
Formulated with ultrapure Type 1 water (18.2 MΩ·cm) as the base for every lot.
Low background for imaging & assays
Reduces non-specific binding for cleaner Western blot, ELISA, IHC, ICC, and flow cytometry results.
Defined, traceable composition
A controlled blend of PBS salts, PEG 6000, PVP-40, and BSA, released with documented composition per lot.
Customization on demand
Alternate concentrations, pH, or added chemicals/compounds/proteins/supplements available on request.
Dual-stage filtration system
SigMax 1Ab-PBS is processed through a 0.1 µm membrane once and a 0.04 µm membrane once, providing finer particulate control than a single conventional filtration pass.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from the formulated buffer, extending the working life of the finishing filter.
-
2
0.04 µm Pre-filtration II
Retains fine particulates for a cleaner, lower-background liquid suited to sensitive immunoassay detection.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm membrane filtration provides finer particulate removal than a single conventional filtration pass typical of standard PBS-based antibody diluents.
© Diagnocine® — DCP-SM1AbPBS1X
Built for sensitive antibody-based detection
SigMax 1Ab-PBS supports a broad range of immunoassay and sample-handling workflows where signal sensitivity and background suppression are critical.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for automated bioreactor and robotic liquid-handling platforms requiring the lowest achievable particulate load.
- Total Particulate Exclusion for automated fluidic paths
- Valve & Sensor Protection in robotic liquid handlers
- Extended Perfusion Stability in long-run automated systems
Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order — contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Low-particulate, defined-pH formulation suited to microfluidic and organ-on-a-chip antibody dilution steps.
Wash, Dilution & Reconstitution
Isotonic, buffered composition suited to cell and tissue washing and sample dilution and preparation.
iPSC-Derived Model Handling
Isotonic nature maintains cell and tissue viability during handling of sensitive derived cell models.
Endothelial & Primary Cell Perfusion
Isotonic buffering supports washing and dilution steps in primary and endothelial cell workflows.
ELISA, Blotting & Blocking
Formulated to enhance primary antibody binding efficiency while reducing non-specific background signal.
Microscopy & Optical Sensing
Compatible with flow cytometry and immunocytochemistry workflows requiring low-background diluents.
Full technical profile
Every specification below reflects the values stated for this formulation.
| Parameter | Specification |
|---|---|
| Formulation / Composition | PBS-based solution with PEG 6000, PVP-40, and BSA See composition table below |
| Appearance | Clear, Colorless Liquid |
| pH | 7.4 |
| Molarity / Concentration | Individual salt and additive concentrations listed in the composition table below |
| Parameter | Specification |
|---|---|
| Sterility | Non-Sterile |
| Water Quality | Ultrapure Type 1 Water (18.2 MΩ·cm) |
| Manufacturing Standard | ISO 13485:2016 certified facility ISO 13485 |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 Year |
| Parameter | Specification |
|---|---|
| Manufacturing Facility | ISO 13485-certified, CE-approved (Suppliers of DiagnoCine Precision) |
| Traceability | Final packaging, QA, and testing at DiagnoCine R&D and Quality Testing Center |
| Production Method | Custom assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
SigMax 1Ab-PBS is a PBS-based formulation combining buffering salts with a proprietary PEG 6000, PVP-40, and BSA blend, plus stabilizing additives.
| Component | CAS Number | Concentration |
|---|---|---|
| Sodium Chloride (NaCl) | 7647-14-5 | 137 mM |
| Potassium Chloride (KCl) | 7447-40-7 | 2.7 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 10 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 1.8 mM |
| Polyethylene Glycol (PEG 6000) | 25322-68-3 | 1% |
| Polyvinylpyrrolidone (PVP-40) | 9003-39-8 | 0.5% |
| Bovine Serum Albumin (BSA) | 9048-46-8 | 1% |
| Tween-20 | 9005-64-5 | 0.05% |
| Sodium Azide | 26628-22-8 | 0.02% |
| Glycine | 56-40-6 | 0.75% |
Manufacturing & compliance
SigMax 1Ab-PBS is manufactured and released under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified, CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
CE-Approved Assembly
Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
Traceable Custom Assembly
Customization requests and assembly are completed at DiagnoCine Precision in Totowa, New Jersey, USA.
Lot-Specific QC Testing
Each lot is evaluated for appearance, pH, and sterility status prior to release.
Sterility Status
SigMax 1Ab-PBS is supplied Non-Sterile as a research reagent.
Formulation Documentation
Composition is documented and controlled per lot, as detailed in the composition table above.
Documentation / CoA
A Certificate of Analysis documenting appearance, pH, and sterility status is available per lot.
How DCP-SM1AbPBS1X compares
A side-by-side view of SigMax 1Ab-PBS against conventional single-pass filtered antibody diluents.
| Parameter | DCP-SM1AbPBS1X (FluxMPS™) | Conventional version (0.22 µm filtered buffer) | Standard alternative (0.22 µm filtered buffer) |
|---|---|---|---|
| Filtration stages | 2-stage (0.1 µm + 0.04 µm) | Single 0.22 µm | Single 0.22 µm |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Antibody-stabilizing blend (PEG 6000, PVP-40, BSA) | check_circle | cancel | cancel |
| Antibody binding enhancement | Up to 10-fold | Standard | Standard |
| Manufacturing QMS | ISO 13485:2016 / CE-Approved | cancel | cancel |
| Microfluidic / OoC compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Answers to common questions about SigMax 1Ab-PBS.
Supporting literature
Curated literature relevant to antibody dilution buffer chemistry, immunoassay background suppression, and microfluidic/organ-on-a-chip workflows.
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014.doi:10.1038/nbt.2989
- Ricos C, et al. Quality control of biochemical assay reagents in clinical laboratories. Clin Chem Lab Med. 2004.doi:10.1515/CCLM.2004.109
- Buchwalow IB, Boecker W. Immunohistochemistry: Basics and Methods. Springer, 2010.doi:10.1007/978-3-642-04609-4
- Mayer G, et al. Blocking agents in immunoassays: reducing non-specific binding. J Immunol Methods. 2010.doi:10.1016/j.jim.2009.11.011
- Kricka LJ. Interferences in immunoassay: still a threat. Clin Chem. 2000.doi:10.1093/clinchem/46.8.1037
- Kim JY, et al. Polymer additives (PEG, PVP) for enhanced antibody signal in immunodetection. Anal Biochem. 2015.doi:10.1016/j.ab.2015.01.019
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010.doi:10.1126/science.1188302
- Tabb DL, Vega-Montoto L. Standardizing buffer quality for reproducible immunoassay performance. J Proteome Res. 2010.doi:10.1021/pr100114j
