UltraClean PVSA-TBE, 1X
Cat No.:
DCP-TBEPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-TBE, 1X is a ready-to-use, high-purity Tris–borate–EDTA buffer formulated to be RNase-safe for RNA and DNA workflows that rely on TBE as the primary buffer system. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, electrophoresis-related processing, and short-term storage. The buffer provides the ionic strength and buffering characteristics of standard 1X TBE, making it suitable for use with nucleic acid samples, gels, and assays where RNA and DNA stability are critical in borate-based conditions.
Applications:
- RNA and DNA Sample Preparation: Dilution and preparation of RNA and DNA samples in a TBE-based buffer with added RNase protection prior to loading on gels or downstream analysis.
- Electrophoresis-Related Workflows: Buffer environment for nucleic acid handling in workflows associated with agarose or polyacrylamide gel electrophoresis when RNA integrity must be maintained.
- RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, purified nucleic acids, or gel-extracted fragments in an RNase-controlled TBE buffer (as compatible with downstream enzymes).
- Probe and Fragment Handling: Dilution and handling of oligonucleotides, probes, and nucleic acid fragments in workflows standardized around TBE rather than other buffer systems.
- General Molecular Biology: Any procedure requiring 1X TBE with enhanced RNA protection, where compatibility with TBE-based electrophoresis and nucleic acid handling protocols is desired.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a broad spectrum of RNases, giving added protection beyond standard nuclease-free handling practices.
- High Purity: Manufactured from molecular biology-grade Tris, borate, EDTA, and PVSA, and finely filtered to minimize particulates, microbial contamination, and adventitious nucleases.
- TBE-Compatible: Formulated to match standard 1X TBE conditions, ensuring compatibility with common gel and nucleic acid handling protocols that specify TBE.
- Nucleic Acid Stability: EDTA chelation and controlled buffering help stabilize nucleic acids while limiting divalent-cation–dependent nuclease activity.
- Ready-to-Use 1X Solution: Supplied at working strength to minimize preparation time, variability, and contamination risk.
Composition:
| Ingredients |
Concentration |
| Tris Base |
89 mM |
| Borate |
89 mM |
| EDTA |
2 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
