PBS with (1%) Tween 20 Buffer [1X]
Cat No.: DCP-PBST20X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane Once and 0.04-micron membrane ONCE.
Description:
PBS Tween 20 buffer is a widely used wash solution for various immunoassays and antibody-based techniques. Tween 20 acts as a non-ionic detergent-based blocking agent, preventing the non-specific binding of antibodies or proteins to surfaces like microplate wells or membranes. This effect reduces background noise and increases the signal of interest. This reduces background noise and improves signal detection in ELISA and Western blotting assays. It is a non-ionic detergent that helps to disrupt non-specific protein-protein interactions during washing steps, enhancing the removal of unbound proteins or antibodies. It also reduces surface tension, allowing for better penetration and removal of unbound molecules.
Advantages:
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It reduces non-specific binding.
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It disrupts protein-protein interactions.
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It improves washing efficiency.
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It promotes reagent spreading.
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It helps to maintain protein stability.
Applications:
Composition:
| Ingredients |
Concentration |
| NaCl |
137 mM |
| KCl |
2.7 mM |
| Na2HPO4 |
10 mM |
| KH2PO4 |
1.8 mM |
| Tween 20 |
1% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron once + 0.04 micron once in a non-sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
