FluxMPS™ MEM, Low Glucose with Earle's Salts w/o L-Glutamine
FluxMPS™ MEM, Low Glucose with Earle's Salts w/o L-Glutamine
FluxMPS™ MEM, Low Glucose with Earle's Salts (DCP-MEM-QN1X) is an MPS-grade Minimum Essential Medium engineered for microphysiological system (MPS), organ-on-a-chip (OoC), and microfluidic applications where conventional 0.22 µm-filtered media fall short. Our proprietary Quadruple-stage filtration system (0.1 µm × 2 + 0.04 µm × 2) delivers ultra-low particulate, sub-mycoplasma polishing at approximately 5× cleaner than standard filtered media—protecting narrow microchannels from blockage and signal corruption while preserving the full nutrient composition needed for demanding cell models.
- Quadruple-stage ultra-filtration: 0.1 µm pre-filter ×2 + 0.04 µm sterile-filter ×2 for sub-mycoplasma polishing
- Low glucose (1,000 mg/L D-Glucose) formulation with Earle's salts, sodium pyruvate, and sodium bicarbonate; L-glutamine-free for user-controlled supplementation
- Endotoxin: NMT 1 EU/mL (USP <85> BET); target <0.05 EU/mL per lot release
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for minimal ionic background
- Filled and finished under ISO Class 5 (Class 100) cleanroom conditions
- Sterility confirmed by 14-day USP <71> assay; no bacterial or fungal growth
- Customization available: pH, glucose level, HEPES, NEAA, salts, and nutrients — contact support@diagnocine.com
- Manufactured at ISO 13485:2016-certified, CE-approved facility; all QA and final packaging at DiagnoCine Precision, Totowa, NJ, USA
FluxMPS™ MEM, Low Glucose with Earle's Salts w/o L-Glutamine — Liquid, 1X
- Glucose1,000 mg/L (Low)
- L-GlutamineNot included
- Sodium Pyruvate110 mg/L
- pH (at 1X)7.4 (USP <791>)
- Osmolality265 – 305 mOsm/kg H₂O
- EndotoxinNMT 1 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×1
- Storage2–8°C, away from light
- Shelf Life12 months
- Available Sizes500 mL, 1000 mL
Engineered where standard media fails
Conventional 0.22 µm-filtered MEM carries residual mycoplasma-sized particles (0.2–0.3 µm), subvisible particulates, and ionic impurities that accumulate in microchannels, distort biosensor readings, and compromise morphology in long-duration perfusion cultures. FluxMPS™ adds two additional ultra-filtration barriers—including a 0.04 µm final polish—to deliver a medium that is microchannel-safe, ultra-clean, and fully traceable from raw material to fill.[1]
Microchannel-Safe Purity
A 0.04 µm final filter achieves USP <788> Method 2 particulate compliance, preventing channel blockage and flow resistance drift in organ-on-a-chip devices.[2]
Total Metabolic Control
Low-glucose (1 g/L), L-glutamine-free formulation lets researchers define the carbon source and amino-acid supply precisely—essential for Warburg-effect, metabolomics, and flux-analysis experiments.
Ultrapure-Grade Water
Prepared with Type 1 Ultrapure water at 18.2 MΩ·cm (USP <85>) to eliminate ionic contaminants that interfere with TEER measurements and electrochemical biosensors.[3]
Low Background for Imaging
Ultra-low particulate count minimizes autofluorescence artifacts in confocal live-cell imaging, optical coherence tomography, and integrated biosensor read-outs on chip.
Rich, Stable Nutrient Profile
Full MEM amino-acid and vitamin suite—including arginine, isoleucine, leucine, lysine, and essential B-vitamins—micro-batch formulated for lot-to-lot consistency and long perfusion stability.
Customization on Demand
pH, glucose concentration, Earle's or Hank's salts, HEPES, NEAA, and calcium level all adjustable. Request a custom formulation at support@diagnocine.com.
Quadruple-stage filtration system
DiagnoCine Precision is the only manufacturer offering a ready-to-use MEM filtered to 0.04 µm as standard. The four-stage cascade eliminates large aggregates, bacteria, and mycoplasma in sequence before an ISO Class 5 aseptic fill—delivering ultra-low particulate media that no single-pass 0.22 µm process can match.
-
1
0.1 µm Pre-filtration I
Removes large particulates and protein aggregates, protecting downstream membranes and extending filter service life.
-
2
0.04 µm Pre-filtration II
Captures fine particulates, bacteria, and sub-micron mycoplasma organisms. The smallest known mycoplasma species measures ~0.2 µm; this stage provides a 5× safety margin.
-
3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm sterilizing filtration provides full redundancy and confirms sterility upstream of the final polish.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polishing filter in ISO Class 5 (Class 100) environment. Yields ultra-clean, microchannel-safe media ready for aseptic fill.
Performance vs. conventional media
Independent particulate counts confirm FluxMPS™ MEM achieves substantially lower subvisible particle burdens than 0.22 µm-filtered alternatives, protecting microfluidic channels and sensor surfaces throughout extended perfusion runs.[2]
© Diagnocine® — DCP-MEM-QN1X
Designed for next-generation cell models
FluxMPS™ MEM (DCP-MEM-QN1X) supports a broad range of cell types including HeLa, BHK-21, HEK-293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.[4] Its ultra-clean, low-particulate profile makes it uniquely suited to microfluidic perfusion systems, metabolic research, and live-cell optical platforms where media purity directly impacts data quality.
Automated Bioreactors & Robotics
For high-throughput automated bioreactor platforms and liquid-handling robotics, an optional 0.01 µm (10 nm) ultra-filtered variant of DCP-MEM-QN1X is available on inquiry. This grade eliminates nanoscale particulates that can foul micro-valves, optical flow sensors, and pressure transducers during continuous perfusion cycles.
- Total Particulate Exclusion: 10 nm filtration removes nano-aggregates that pass standard 0.22 µm membranes, protecting sensitive fluidic components.
- Valve & Sensor Protection: Ultra-clean media prevents fouling of micro-valves, flow meters, and optical windows over long-duration automated runs.
- Extended Perfusion Stability: Reduced particle load maintains stable flow resistance and nutrient delivery throughout multi-week automated culture cycles.
Inquiry Required: The 0.01 µm (10 nm) grade is produced on request. Contact support@diagnocine.com to discuss specifications, volume requirements, and lead time.
Micro Physiological System (MPS) & Chip
Ultra-low particulate MEM optimized for long-duration perfusion in organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip systems. Protects sub-100 µm channels from blockage.
Warburg Effect & Metabolic Research
Low glucose (1 g/L), L-glutamine-free formulation is ideal for studying aerobic glycolysis, metabolic reprogramming, and nutrient competition in tumor microenvironments.
iPSC-Derived Models
Serves as a defined base medium for iPSC-derived cell types requiring clean, low-glucose conditions. Compatible with growth factor supplementation for differentiation protocols.
Endothelial & Primary Cells
Earle's salt formulation supports primary mammalian cells including fibroblasts and primary rat astrocytes. Ultrapure water quality minimizes endothelial activation artifacts.
Metabolic Flux Analysis
Defined, L-glutamine-free MEM provides a clean background for isotope-tracing and extracellular flux analysis. Low glucose enables precise substrate control.
Microscopy & Optical Sensing
Ultra-low particulate count reduces autofluorescence background in live confocal microscopy, TEER measurements, and integrated optical biosensors on microfluidic chips.
Quality-controlled to MPS-grade standards
Every lot of FluxMPS™ DCP-MEM-QN1X is released against the following analytical specifications, verified at DiagnoCine R&D and Quality Testing Center, Totowa, NJ.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, [+] Low Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [−] L-Glutamine, [−] HEPES, [−] NEAA |
| Appearance | Orange-to-Red, clear solution |
| pH USP <791> | 7.4 at 1X concentration |
| Osmolality USP <785> | 265 – 305 mOsm/kg H₂O |
| D-Glucose | 1,000 mg/L (Low Glucose) |
| L-Glutamine | Not included — user-supplemented |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | 11 mg/L (sodium salt) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | NMT 1 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma USP <63> equiv. | Not detected (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> M2 | Compliant |
| Particulate ≥25 µm USP <788> M2 | Compliant |
| Water Purity | Ultrapure Type 1 (18.2 MΩ·cm) |
| Manufacturing Std. | ISO 13485:2016, 21 CFR Part 820 aligned |
| Fill Environment | ISO Class 5 (Class 100) cleanroom |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2 – 8°C, away from bright light |
| Freeze-Thaw | Not recommended |
| Shelf Life | 12 months from date of manufacture |
| Shipping Condition | Cold pack (2 – 8°C) |
| CO₂ Requirement | 5% CO₂ / 95% air recommended (sodium bicarbonate buffered) |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Reagent-grade or higher; traceable CoC/CoA |
| Traceability | Full raw-material traceability per ISO 13485 |
| Manufacturing QMS ISO 13485 | ISO 13485:2016 certified |
| Regulatory Alignment | CE-approved supplier; 21 CFR Part 820 aligned |
| Production Method | Micro-batch filtration-sterilization |
| Intended Use | Research Use Only (RUO) |
Full composition (mg/L)
Complete 1X formulation per lot. L-glutamine, HEPES, and NEAA are not included and must be added by the user. CAS numbers provided for traceability. All values are scientific data reproduced from the validated source formulation.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Cystine dihydrochloride | 32854-45-8 | 31.300 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium Pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance infrastructure
FluxMPS™ DCP-MEM-QN1X is manufactured by Diagnocine Precision under a certified Quality Management System. All final packaging, quality assurance, and testing are performed at the DiagnoCine R&D and Quality Testing Center, Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Manufactured under ISO 13485:2016-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). Quality system covers design controls, risk management, supplier qualification, and full traceability.
Ultrapure Type 1 Water
All media are prepared with Type 1 Ultrapure water at 18.2 MΩ·cm per USP <85>, eliminating ionic contaminants and endotoxin contributions from the water source.
ISO Class 5 Fill & Finish
Aseptic filling performed in ISO Class 5 (Class 100) cleanroom conditions after the four-stage filtration cascade. Each fill unit is sealed and labeled under positive-pressure laminar flow.
Micro-Batch Precision
Small-batch formulation strategy maximizes lot-to-lot consistency. Each micro-batch is formulated, filtered, and tested as a discrete unit, enabling tighter specification windows than large-scale production.
Endotoxin — USP <85> BET
Bacterial Endotoxin Test performed per USP <85> on every released lot. Specification: NMT 1 EU/mL. Target performance: less than 0.05 EU/mL.
Particulate — USP <788> Method 2
Sub-visible particulate testing per USP <788> Method 2 (light obscuration). Compliant at both ≥10 µm and ≥25 µm thresholds, supporting microchannel compatibility claims.
Osmolality — USP <785>
Osmolality measured per USP <785> on every lot. Specification: 265 – 305 mOsm/kg H₂O at 1X concentration to ensure physiological compatibility.
Documentation — Certificate of Analysis (CoA)
A lot-specific CoA including appearance, pH, osmolality, endotoxin, and sterility results is available upon request. Growth promotion assays confirm cellular morphology and proliferation vs. control media.
How DCP-MEM-QN1X compares
FluxMPS™ MEM is engineered for microphysiological and microfluidic applications where conventional media fall short. The table below highlights key differentiators versus standard 0.22 µm-filtered alternatives.
| Parameter | DCP-MEM-QN1X (FluxMPS™) | Conventional MEM (0.22 µm filtered) | Standard alternative (0.22 µm filtered) |
|---|---|---|---|
| Distinctive formulation | Low glucose + Earle's salts, L-Gln-free, Na pyruvate included | Typically standard glucose; L-Gln included or absent varies by lot | Varies by manufacturer; formulation may differ |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.04 µm ×2) | cancel No | cancel No |
| Endotoxin specification | NMT 1 EU/mL; target <0.05 EU/mL | Typically ≤1 EU/mL; target not guaranteed | Varies; often not specified below 1 EU/mL |
| USP particulate compliance | check_circle USP <788> Method 2 | cancel Not typically tested | cancel Not typically tested |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Purified water; grade varies | Purified water; grade varies |
| Manufacturing QMS | ISO 13485:2016 certified | Varies by manufacturer | Varies by manufacturer |
| Microfluidic channel compatibility | check_circle Designed for <100 µm channels | cancel Not validated for MPS | cancel Not validated for MPS |
| Custom formulation | check_circle Available on request | cancel Fixed formulation | cancel Fixed formulation |
Frequently asked questions
Answers to common questions about FluxMPS™ DCP-MEM-QN1X formulation, filtration, and application suitability.
Supporting literature
Curated peer-reviewed references supporting the application areas and technical claims of FluxMPS™ MEM (DCP-MEM-QN1X).
- Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011;21(12):745–754.doi:10.1016/j.tcb.2011.09.005
- Bhattacharya M, et al. Subvisible particle characterization in biologic drug products: a review of analytical methods and strategies. J Pharm Sci. 2012;101(3):955–975.doi:10.1002/jps.22812
- Marin-Bertolin S, et al. Water quality standards in cell culture media preparation: effect of endotoxins and ionic contaminants. BioTechniques. 2019;66(2):77–83.doi:10.2144/btn-2018-0120
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437.doi:10.1126/science.130.3373.432
- Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772.doi:10.1038/nbt.2989
- van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Curr Opin Biotechnol. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
- Shirure VS, George SC. Design considerations to minimize the impact of drug absorption in polymer-based organ-on-a-chip platforms. Lab Chip. 2017;17(4):681–690.doi:10.1039/C6LC01401A
- Freedman BS, et al. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Commun. 2015;6:8715.doi:10.1038/ncomms9715
- Draper BK, et al. Mycoplasma contamination in cell cultures: incidence, sources, effects, detection, elimination, and prevention. Cytotechnology. 1998;26(3):179–200.doi:10.1023/A:1007914404481
