FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Phenol Red: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Phenol Red: 1X Liquid
FluxMPS™ DCP-MEM-R1X is a Microfluidics Suitable, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Phenol Red.
- MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
- NEAA included (Ala, Asn, Asp, Glu, Pro) — reduces de novo synthesis burden on primary cells cultured in low-serum conditions
- Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
- 0.04 µm final nano-filtration — sub-mycoplasma-scale purity; endotoxin release specification < 0.05 EU/mL
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final Filtration I → 0.1 µm Prefiltration II → 0.04 µm Final Filtration II — Polish
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
- Custom formulation modifications (pH, glucose, NEAA concentration, HEPES) available on request
Available sizes: 500 mL, 1000 mL
- BaseMEM + Earle’s Salts + NEAA
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- Formulation[+] Earle's Salts, [+] NEAA, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Sodium Pyruvate | [-] Phenol Red
- pH (USP <791>)7.4
- Osmolality (USP <785>)290–330 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered for primary cell culture where standard media fails
MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ is engineered to address these failure modes while preserving the full MEM + NEAA nutritional profile that primary cells depend on.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.
Primary cell–optimized formulation
MEM + Earle’s Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) — formulated with tight trace-metal and organic-carbon (TOC) control to minimize adventitious contaminants in sensitive primary cell culture.
Low endotoxin release specification
< 0.05 EU/mL release specification, tested per batch — formulated to reduce the risk of LPS-driven inflammatory activation that can confound fibroblast-to-myofibroblast transition and neuronal inflammatory response assays.
NEAA reduces metabolic burden
Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.
Customization on demand
pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — a repeated prefilter and final-filter pair, run twice — reaching a final 0.04 µm polish. For primary cell culture, sub-mycoplasma-scale filtration is especially important: mycoplasma contamination in MEM has been associated with altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates, cell debris, and protein contaminants; protects the downstream 0.04 µm cartridge and chip microchannel geometries from fouling.
-
2
0.04 µm Final Filtration I — Mycoplasma-Range Barrier
First 0.04 µm pass; retains particulates in the mycoplasma size range (0.2–0.3 µm) and sub-micron aggregates not addressed by standard 0.22 µm filtration.
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3
0.1 µm Prefiltration II — Redundant Protection
A second, dedicated 0.1 µm prefilter protects the second 0.04 µm cartridge, providing full redundancy ahead of the final polish.
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4
0.04 µm Final Filtration II — Polish
Ultimate sub-mycoplasma-scale polishing filtration; ISO Class 5 aseptic fill & finish.
Performance vs. conventional MEM
© Diagnocine® — DCP-MEM-R1X
Primary cell models & OoC applications
FluxMPS™ DCP-MEM-R1X is purpose-built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates cause accelerated chip fouling — a separate tier from the Microfluidics Suitable product described on this page.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
- Valve & Sensor Protection: Reduces micro-fouling risk in delicate neuronal and epithelial chip geometries
- Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Neuronal Chips & Brain-on-Chip
MEM + Earle’s Salts + NEAA + low glucose is a physiologically appropriate base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.
Epithelium-on-Chip
Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and barrier integrity in transwell and microfluidic TEER-monitored platforms.
Primary Fibroblast & Stromal Models
MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation associated with myofibroblast differentiation artefacts.
Vascular Cell Culture
Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.
Organ-on-a-Chip & MPS
0.04 µm filtered MEM reduces the risk of particulate clogging in sub-100 µm neuronal and epithelial chip microchannels compared with standard 0.22 µm filtered MEM.
Microscopy & Optical Sensing
Ultra-low particulate, phenol red–free MEM reduces background autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Phenol Red |
| Appearance | Pale yellow-colored, clear solution |
| Base | MEM + Earle’s Salts + NEAA |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 290–330 mOsm/kg H₂O |
| Total ingredients | 36 across 4 composition categories |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see §Quality Assurance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm + 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MEM with Earle’s Salts and NEAA: 36 ingredients across 4 composition categories, verified per lot with CAS numbers, organized below into 3 navigable tabs. The Amino Acids tab includes both essential amino acids (EAA) and Non-Essential Amino Acids (NEAA) as one combined group, per the source composition table.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| i-Inositol | 87-89-8 | 2.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular care for primary cell–grade purity standards.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with tight trace-metal and organic-carbon (TOC) control.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 290–330 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-MEM-R1X compares
FluxMPS™ DCP-MEM-R1X vs. conventional 0.22 µm–filtered MEM and standard high-glucose DMEM for primary cell OoC applications.
| Parameter | DCP-MEM-R1X (FluxMPS™) | Conventional MEM (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm) | Not applicable (0.22 µm filtered) | |
| Full-formulation MEM with Earle's Salts and NEAA, without Phenol Red | check_circle Yes | cancel No | cancel No |
| Salt formulation | Earle’s Salts (5% CO₂ optimized) | Earle’s Salts | Modified Earle’s |
| NEAA included | check_circle Yes (Ala, Asn, Asp, Glu, Pro) | Optional add-on | cancel Not included |
| Glucose | 1.0 g/L (Low — physiological) | 1.0 g/L | 4.5 g/L (High) |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-range barrier filtration | check_circle Yes (0.1 µm + 0.04 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel No | cancel No |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Not specified | Not specified |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Yes — Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-MEM-R1X — MEM with Earle’s Salts and NEAA.
Supporting literature
Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
