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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ DCP-MEM-QPBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered MEM Low Glucose + NEAA formulation engineered for primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded, reducing metabolic burden on primary cells. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L) | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red.
- Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose media.
- NEAA included — reduces metabolic burden and ammonia accumulation from de novo amino acid synthesis.
- 0.04 µm final nano-filtration — sub-mycoplasma-scale purity; release specification < 0.05 EU/mL endotoxin (USP <85>) for sensitive primary and hematopoietic cell cultures.
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish.
- Bicarbonate-free and phenol-red-free formulation — CO₂-independent buffering; supplement with HEPES (15–25 mM) if additional pH stability is required.
- Manufactured under an ISO 13485:2016 quality management system; final QA release at Diagnocine's Totowa, NJ facility.
- Microfluidics Suitable (0.04 µm final cut-off) — engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms; a separate 0.01 µm MPS Grade line is available on request.
- Media familyMEM Low Glucose + NEAA
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose
[-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red - AppearanceColorless, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered for primary cell culture where standard media fails
MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin that alter primary cell phenotype. FluxMPS™ addresses these purity failure modes while preserving the full nutritional profile primary cells depend on.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate media helps prevent chip channel clogging in narrow microfluidic geometries.
Primary cell–optimized formulation
MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) provides tight trace-metal and total organic carbon (TOC) control, supporting lot-to-lot consistency in primary cell culture performance.
Below the TLR4 endotoxin threshold
Release specification < 0.05 EU/mL endotoxin, below the conventional TLR4 activation threshold, helping minimize the risk of LPS-driven fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption in primary cell assays.
NEAA reduces metabolic burden
Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.
Customization on demand
pH, glucose, HEPES concentration, and other component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I
Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-MEM-QPBR1X
Primary cell models and OoC applications
FluxMPS™ DCP-MEM-QPBR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request — the separate ultra nano-filtered tier described in the Purity Architecture note above.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates.
- Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries.
- Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture.
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Neuronal Chips & Brain-on-Chip
MEM + Earle's Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.
Epithelium-on-Chip
Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.
Primary Fibroblast & Stromal Models
MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.
Vascular Cell Culture
Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.
Organ-on-a-Chip & MPS
0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.
Microscopy & Optical Sensing
Ultra-low particulate, phenol-red-free formulation minimizes autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L) | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red |
| Appearance | Colorless, clear solution |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| NEAA | Present |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 33 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering |
| Available pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MEM Low Glucose + NEAA: 33 ingredients verified per lot with CAS numbers. NEAA is listed under AMINO ACIDS.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine's R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — tight trace-metal and TOC control.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-MEM-QPBR1X compares
FluxMPS™ DCP-MEM-QPBR1X vs. conventional 0.22 µm-filtered MEM Low Glucose + NEAA formulations, and against published supplier endotoxin specifications.
| Parameter | DCP-MEM-QPBR1X (FluxMPS™) | Conventional MEM Low Glucose (0.22 µm filtered) | Standard DMEM (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Most minimal MEM — CO₂-independent, all metabolic inputs researcher-defined; NEAA and low glucose only | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| NEAA included | check_circle Yes | Optional add-on | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Yes — Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-MEM-QPBR1X.
Supporting literature
Key publications supporting MEM Low Glucose + NEAA in primary cell OoC applications.
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
